An experiment based on physiological parameters and subjective emotional changes is designed to analyze the sound‐light fusion effects. The different effects caused by music in major and minor keys is studied. Three colorful lighting with main wavelength at 623, 537, 445 nm, respectively, as well as two white lighting with 3000 K CCT and 6000 K CCT are evaluated in the same process. Results show that the physiological response to music is stronger than that of the lighting stimulus. Music plays a major role in the sound‐light fusion environment, and the physiological perception under dual‐factor stimulation is stronger than that of single factor. Moreover, music and light interact with each other when they stimulate emotions at the same time. The superposition of two positively related factors can strengthen the effects on emotion. The studies may give suggestions to the designers when they design a lighting show with music or an immersive and interactive lighting project.