Analysis of repeated compound units in ORF94 of white spot syndrome virus isolated from Litopenaeus vannamei from outbreak and non outbreak shrimp farms in Sonora, Mexico

Rodriguez-Anaya, Libia Zulema; Ibarra‐Gámez, Jose Cuauhtémoc; Lares‐Villa, Fernando; Casillas-Hernández, Ramón; Sánchez-Flores, Alejandro; Gonzalez-Galaviz, Jose Reyes

doi:10.3856/vol44-issue4-fulltext-21

Cited by 2 publications

(1 citation statement)

References 19 publications

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“…() and Rodríguez‐Anaya et al . (), who found a higher prevalence of WSSV variants with fewer RUs in ORF94 in ponds with disease outbreaks. Cumulative mortality percentage data segregated the four WSSV strains into three virulence levels, where only the virulence of the Mx‐G, Mx‐F and Mx‐C strains correlated inversely with the length of the ORF94 VNTR.…”

Section: Discussionmentioning

confidence: 94%

Virulence and genotypes of white spot syndrome virus infecting Pacific white shrimp Litopenaeus vannamei in north‐western Mexico

Ramos-Paredes

Chon

Ibarra-Gámez

2017

Journal of Fish Diseases

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White spot syndrome virus (WSSV) has caused substantial global economic impact on aquaculture, and it has been determined that strains can vary in virulence. In this study, the effect of viral load was evaluated by infecting Litopenaeus vannamei with 10-fold serial dilution of tissue infected with strain WSSV Mx-H, and the virulence of four WSSV strains from north-western Mexico was assessed along with their variable number of tandem repeat (VNTR) genotypes in ORF75, ORF94 and ORF125. The LD of the Mx-H strain was a dilution dose of 10 ; the mortality titre was 10 LD per gram. In shrimp injected with 10 to 10 LD , no significant virulence differences were evident. Using mortality data, the four WSSV strains grouped into three virulence levels. The Mx-F strain (intermediate virulence) and the Mx-C strain (high virulence) showed more genetic differences than those observed between the Mx-G (low-virulence) and Mx-H (high-virulence) strains, in ORF94 and ORF125. The application of high-viral-load inocula proved useful in determining the different virulence phenotypes of the WSSV strains from the Eastern Pacific.

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Section: Discussionmentioning

confidence: 94%

Virulence and genotypes of white spot syndrome virus infecting Pacific white shrimp Litopenaeus vannamei in north‐western Mexico

Ramos-Paredes

Chon

Ibarra-Gámez

2017

Journal of Fish Diseases

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Virulence and genetic differences among white spot syndrome virus isolates inoculated in Penaeus vannamei

et al. 2022

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White spot syndrome virus (WSSV) infects several economically important aquaculture species, and has caused significant losses to the industry. This virus belongs to the Nimaviridae family and has a dsDNA genome ranging between 257 and 309 kb (more than 20 isolate genomes have been fully sequenced and published to date). Multiple routes of infection could be the cause of the high virulence and mortality rates detected in shrimp species. Particularly in Penaeus vannamei, differences in isolate virulence have been observed, along with controversy over whether deletions or insertions are associated with virulence gain or loss. The pathogenicity of 3 isolates from 3 localities in Mexico (2 from Sinaloa: ‘CIAD’ and ‘Angostura’; and one from Sonora: ‘Sonora’) was evaluated in vivo in whiteleg shrimp P. vannamei infection assays. Differences were observed in shrimp mortality rates among the 3 isolates, of which Sonora was the most virulent. Subsequently, the complete genomes of the Sonora and Angostura isolates were sequenced in depth from infected shrimp tissues and assembled in reference to the genome of isolate strain CN01 (KT995472), comprising 289350 and 288995 bp, respectively. Three deletion zones were identified compared to CN01, comprising 15 genes, including 3 envelope proteins (VP41A, VP52A and VP41B), 1 non-structural protein (ICP35) and 11 other encoding proteins whose function is currently unknown. In addition, 5 genes (wsv129, wsv178, wsv204, wsv249 and wsv497) presented differences in their repetitive motifs, which could potentially be involved in the regulation of gene expression, causing virulence variations.

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Analysis of repeated compound units in ORF94 of white spot syndrome virus isolated from Litopenaeus vannamei from outbreak and non outbreak shrimp farms in Sonora, Mexico

Cited by 2 publications

References 19 publications

Virulence and genotypes of white spot syndrome virus infecting Pacific white shrimp Litopenaeus vannamei in north‐western Mexico

Virulence and genotypes of white spot syndrome virus infecting Pacific white shrimp Litopenaeus vannamei in north‐western Mexico

Virulence and genetic differences among white spot syndrome virus isolates inoculated in Penaeus vannamei

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