Analysis of Sogatella furcifera proteome that interact with P10 protein of Southern rice black-streaked dwarf virus

Than, Win Win; Qin, Faliang; Liu, Wenwen; Wang, Xifeng

doi:10.1038/srep32445

Cited by 18 publications

(22 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As with virus infection, extreme temperatures, or insecticide treatment, each stress could lead to the change in the expression patterns in WBPH, and these results provide a valuable resource for the molecular characterization of stress action in WBPH [ 17 , 25 , 34 ]. Moreover, Than et al investigated the protein–protein interactions between SRBSDV and WBPH [ 35 ]. They identified over 100 WBPH proteins as putative interactors of the coat protein of SRBSD, which helped increase the understanding about how SRBSDV affects its insect vector.…”

Section: Discussionmentioning

confidence: 99%

Metabolomic Changes in Sogatella furcifera under Southern rice black-streaked dwarf virus Infection and Temperature Stress

Zhang

Feng

et al. 2018

Viruses

View full text Add to dashboard Cite

Southern rice black-streaked dwarf virus (SRBSDV) is a devastating newly emerged rice reovirus in Eastern and Southeastern Asia transmitted by a long-distance migratory pest, the white-backed planthopper (WBPH). We previously showed that SRBSDV infection decreased the cold tolerance but improved the heat tolerance of its vector, WBPH. Comparative metabolomic analysis was used to explore the potential mechanisms underlying these changes in temperature stress response. Fourth-generation WBPH nymphs were treated with SRBSDV and/or extreme temperature stress and were analyzed using gas chromatography-time of flight-mass spectrometry. A total of 605 distinguishable peaks were identified and 165, 207, and 202 differentially accumulated metabolites were identified in WBPH after virus infection, cold, or heat stress, respectively. The nucleic acids and fatty acids were the major categories of metabolites regulated by SRBSDV infection, whereas temperature stress regulated tricarboxylic acid cycle compounds, sugars, and polyols. For the WBPH samples infected with SRBSDV and subjected to temperature stress, amino acids, sugars, and polyols were the most significant regulated metabolites. The metabolomics study suggests that SRBSDV may influence the extreme temperature tolerance of WBPH by regulating the accumulation of amino acids, sugars, and polyols in the insect body.

show abstract

Section: Discussionmentioning

confidence: 99%

Metabolomic Changes in Sogatella furcifera under Southern rice black-streaked dwarf virus Infection and Temperature Stress

Zhang

Feng

et al. 2018

Viruses

View full text Add to dashboard Cite

show abstract

“…qRT-PCR was performed using the Roche LightCycler ® 480II. The thermal cycling program was as follows5758: 95 °C for 15 min, 40 cycles at 95 °C for 15 s and at 60 °C for 30 s. The gene expression of rice UBQ5 was used as an internal control.…”

Section: Methodsmentioning

confidence: 99%

Proteomic and Carbonylation Profile Analysis at the Critical Node of Seed Ageing in Oryza sativa

Yin

Xia

et al. 2017

Sci Rep

View full text Add to dashboard Cite

The critical node (CN), which is the transition from the plateau phase to the rapid decreasing phase of seed ageing, is extremely important for seed conservation. Although numerous studies have investigated the oxidative stress during seed ageing, information on the changes in protein abundance at the CN is limited. In this study, we aimed to investigate the abundance and carbonylation patterns of proteins at the CN of seed ageing in rice. The results showed that the germination rate of seeds decreased by less than 20% at the CN; however, the abundance of 112 proteins and the carbonylation levels of 68 proteins markedly changed, indicating oxidative damage. The abundance and activity of mitochondrial, glycolytic, and pentose phosphate pathway proteins were reduced; consequently, this negatively affected energy production and germination. Proteins related to defense, including antioxidant system and heat shock proteins, also reduced in abundance. Overall, energy metabolism was reduced at the CN, leading to a decrease in the antioxidant capacity, whereas seed storage proteins were up-regulated and carbonylated, indicating that the seed had a lower ability to utilize seed storage proteins for germination. Thus, the significant decrease in metabolic activities at the CN might accelerate the loss of seed viability.A notable characteristic of seed viability is the reverse S-shaped survival curve during ageing, which includes a plateau phase (Phase I; P-I), followed by a rapid decreasing phase (Phase II; P-II) and a slow decreasing phase (Phase III; P-III). The transformation from P-I to P-II is defined as the critical node (CN), which is highly important for seed conservation 1 . The average germination of approximately 42,000 diverse accessions stored for 16 to 81 years at the National Center for Genetic Resources Preservation, USA has been decreased by 42% 2 . The average germination rate of peanut (stored for 34 years), soybean (stored for 36 years), wheat (stored for 43.6 years), and barley (stored for 44.4 years) is 6%, 21%, 73%, and 86%, respectively 3 . Similar results have been also reported by the Genebank of the Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Germany 4 . Rice is extremely important food crop. One of main aims in genebanks is maintaining the rice seed safe conservation. At the T.T. Chang Genetic Resources Center in International Rice Research Institute, 183 rice accessions stored up to 30 years showed more than 70% germination 5 , and more than 93% of seed lots produced in 1980 still maintained 85% germination after 33 years in storage 6 . Owing to the reduction in seed viability, the regeneration of genetic resources is considered crucial for maintaining genetic integrity. Previous studies have shown that seed regeneration needs to be carried out prior to the CN in order to prevent a large decrease in viability, which can lead to changes in genetic composition 7,8 . Previously, we showed that the mitochondrial ultrastructure of seed at the CN is abnormal owing to the decre...

show abstract

“…To verify the presence of viruses in field samples, we used RT-PCR and specific primers designed on the sequences of assembled contigs (Table S4 ). The sequences of the extreme ends of the genomic RNAs were determined employing the 5′- and 3′-RACE System for Rapid Amplification of cDNA Ends kits (Thermo Fisher Scientific, Waltham, MA, USA) (Than et al, 2016 ). The primers and other oligonucleotides used for RT-PCR and RACE analyses are listed in Table S3 .…”

Section: Methodsmentioning

confidence: 99%

Two Negative-Strand RNA Viruses Identified in Watermelon Represent a Novel Clade in the Order Bunyavirales

et al. 2017

Self Cite

View full text Add to dashboard Cite

Two novel negative-sense, single-stranded (ss) RNA viruses were identified in watermelon plants and named watermelon crinkle leaf-associated virus 1 and 2 (WCLaV-1 and -2), respectively. The multipartite genomes consist of three RNA molecules of ~6.8, 1.4, and 1.3 kb. The genomes and the deduced proteins of RNA1 and RNA3 show features resembling those of members in the genus Phlebovirus and Tenuivirus; however, the predicted proteins encoded by RNA2 are related to the movement protein (MP) in the genus Ophiovirus and Emaravirus. Furthermore, these two viruses define a novel clade in the family Phenuiviridae, order Bunyavirales, which is phylogenetically related to the viruses in the above four genera. Moreover, after mechanical inoculation with WCLaV-1 seedlings of the natural host watermelon plants develop crinkling similar to those observed in the field. These findings enhance our understanding of the evolution and the classification of ssRNA viruses.

show abstract

Analysis of Sogatella furcifera proteome that interact with P10 protein of Southern rice black-streaked dwarf virus

Cited by 18 publications

References 57 publications

Metabolomic Changes in Sogatella furcifera under Southern rice black-streaked dwarf virus Infection and Temperature Stress

Metabolomic Changes in Sogatella furcifera under Southern rice black-streaked dwarf virus Infection and Temperature Stress

Proteomic and Carbonylation Profile Analysis at the Critical Node of Seed Ageing in Oryza sativa

Two Negative-Strand RNA Viruses Identified in Watermelon Represent a Novel Clade in the Order Bunyavirales

Contact Info

Product

Resources

About