Analysis of steroids from normal and tumor tissue by HPLC

Kessler, Michael J.

doi:10.1016/0009-8981(82)90041-9

Cited by 7 publications

(6 citation statements)

References 5 publications

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“…5a-Reduced C 19 steroids and E2 were found in considerably lower levels demonstrating low or missing activities of the 5a-reductase and aromatase enzymes in this ovarian tumor. Tumor concentrations of 17-OH-Prog, 4-endione, and Test are found very similar with those measured by Kessler [14] with HPLC -RIA and HPLC -ultraviolet spectrometry in a histologically unidentified ovarian tumor. (17-OH-Prog, 4-en-dione, and Test concentrations published by Kessler were 6300, 3000, and 5700 fmol/g, respectively).…”

Section: Discussionsupporting

confidence: 65%

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HPLC–RIA analysis of steroid hormone profile in a virilizing stromal tumor of the ovary

Szécsi

Tóth

Gardi

et al. 2004

Journal of Biochemical and Biophysical Methods

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Section: Discussionsupporting

confidence: 65%

“…Steroid profile studies of human tissues were primarily focussed on the androgen action in prostate tumors [8 -11]. In some cases, hormone metabolism in the brain [12,13], adrenal gland, and ovary [14], as well as steroid levels in blood [15 -17], was investigated with HPLC -RIA.…”

Section: Introductionmentioning

confidence: 99%

HPLC–RIA analysis of steroid hormone profile in a virilizing stromal tumor of the ovary

Szécsi

Tóth

Gardi

et al. 2004

Journal of Biochemical and Biophysical Methods

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“…Analytical procedures currently available for the quantitative analysis of steroidal compounds include radioimmunoassay (16,17), gas chromatography/mass spectrometry (GC/MS) (18 -20), high-performance liquid chromatography (21), and liquid chromatography/ mass spectrometry (22)(23)(24). While these techniques have been successfully employed in the determination of selected steroids in given tissues and/or biological fluids, quantitative methods for the analysis of neurosteroids, especially 5␣,3␣-THP in a reasonable volume of cerebrospinal fluids (CSF) have been difficult due to low CSF concentrations of these compounds.…”

mentioning

confidence: 99%

Profiling Neurosteroids in Cerebrospinal Fluids and Plasma by Gas Chromatography/Electron Capture Negative Chemical Ionization Mass Spectrometry

Kim

Zhang

Kim

2000

Analytical Biochemistry

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“…Since the 1950s, numerous C 21 and C 19 steroids have been isolated from the extracts of human adrenal glands 19,21–24 . These studies confirmed that the adrenal possesses the capability to produce a variety of steroids.…”

Section: Discussionmentioning

confidence: 75%

Liquid chromatography–tandem mass spectrometry analysis of human adrenal vein corticosteroids before and after adrenocorticotropic hormone stimulation

Nakamura

Rege

Satoh

et al. 2012

Clinical Endocrinology

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Context Although steroid hormones produced by the adrenal gland play critical roles in human physiology, a detailed quantitative analysis of the steroid products has not been reported. The current study uses a single methodology (liquid chromatography-tandem mass spectrometry, LC-MS/MS) to quantify ten corticosteroids in adrenal vein (AV) samples pre and post adrenocorticotropic hormone (ACTH) stimulation. Design/methods Three men and six women with a diagnosis of an adrenal aldosterone-producing adenoma (APA) were included in the study. Serum was collected from the iliac vein (IV) and the adrenal vein (AV) contralateral to the diseased adrenal. Samples were collected, before and after administration of ACTH. LC-MS/MS was then used to quantify serum concentrations of unconjugated corticosteroids and their precursors. Results Prior to ACTH stimulation the four most abundant steroids in AV were cortisol (90%), cortisone (4%), corticosterone (3%) and 11-deoxycortisol (0.8%). Post ACTH administration, cortisol remained the major adrenal product (79%), however, corticosterone became the second most abundantly produced adrenal steroid (11%) followed by pregnenolone (2.5%) and 17α-hydroxypregnenolone (2%). ACTH significantly increased the absolute adrenal output of all ten corticosteroids measured (P<0.05). The four largest post ACTH increases were pregnenolone (300-fold), progesterone (199-fold), 17α-hydroxypregnenolone (187-fold) and deoxycorticosterone (82-fold). Conclusion Using LC-MS/MS we successfully measured 10 corticosteroids in peripheral and adrenal vein serum samples under pre and post ACTH stimulation. This study demonstrates the primary adrenal steroid products and their response to ACTH.

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Analysis of steroids from normal and tumor tissue by HPLC

Cited by 7 publications

References 5 publications

HPLC–RIA analysis of steroid hormone profile in a virilizing stromal tumor of the ovary

HPLC–RIA analysis of steroid hormone profile in a virilizing stromal tumor of the ovary

Profiling Neurosteroids in Cerebrospinal Fluids and Plasma by Gas Chromatography/Electron Capture Negative Chemical Ionization Mass Spectrometry

Liquid chromatography–tandem mass spectrometry analysis of human adrenal vein corticosteroids before and after adrenocorticotropic hormone stimulation

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