Analysis of the cellular composition of the native auto-transplantation mixture used for plastics of bone tissue defects

Чуешова, Н. В.; Чешик, И. А.; Надыров, Э. А.; Николаев, В. И.; Kirilenko, S. I.; Рожин, В. В.; Кондрачук, А. Н.; Serdyuchenko, N. S.

doi:10.29235/1561-8323-2021-65-6-715-723

Cited by 1 publication

(2 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…НТА получали с помощью устройства для фильтрации костной стружки путем фильтрации аспирата из раны, образующегося при резекции костной ткани высокооборотистой костной хирургической фрезой [4,5]. Забор КЧ проводили путем резекции костной ткани кусачками Керрисона с рабочей частью 3 мм и представляли из себя фрагменты кортикальной и губчатой костной ткани в форме прямоугольников.…”

Section: материал и методыunclassified

“…BY №22923). При данном способе получаем НТА в виде пластичной гетерогенной массы красного цвета, состоящей из фрагментов разрушенной кости, красного и желтого костного мозга, соединительной ткани [5]. Такой трансплантат по морфофункциональному составу и способом получения значитель-но отличается от других форм местного костного трансплантата.…”

unclassified

See 1 more Smart Citation

Cell Survival Investigation of Two Types of Autological Osteoplastic Materials in Spine Fusion Procedures

Rozhin¹,

Chueshova²,

Nadyrov³

et al. 2022

Vestnik VSMU

View full text Add to dashboard Cite

Objectives. To compare the survival rate of «bone chip» cells and native transplant automixture by evaluating the architectonics of cell membranes. Material and methods. Autograft in the form of a native graft automixture obtained using a «bone dust filtering device» and «bone chips» obtained using Kerrison rongeurs in 6 patients operated for degenerative diseases of the spine. The study was carried out using a flow cytometer by assessing the architectonics of cell membranes during the first three days. Results. The study of native transplant automixture and «bone chips» determined the dispersed state of the first graft and the latter, similar in its structure to the whole bone. The spectrograms analysis showed a heterogeneous composition of grafts, and in the case of native transplant automixture, the scatter was more pronounced. The increase in the number of cells during the first three days of the native transplant automixture is probably associated with the elimination of contact inhibition of their proliferation and the release of osteoinductive factors from the bone matrix. The number of necrotic cells was higher in the native transplant automixture, which is due to the rough method of its preparation, however, this number was not statistically significant compared to the number of necrotic cells in the «bone chips». Despite the combined thermal and mechanical impact, the survival of the cell component in both cases made up more than 98.0% without any statistical significance in comparison between the two types of transplants (p>0.05). Conclusions. The high survival rate of the cellular component of both types of autografts indicates that the osteogenic potential does not depend on the method of their preparation.

show abstract

Section: материал и методыunclassified

unclassified