Analysis of the differential expression profile of miRNAs in myocardial tissues of rats with burn injury

Guo, Jingdong; Zhu, Zhensen; Zhang, Dongmei; Chen, Bin; Zou, Ben Yan; Gao, Songying; Zhu, Xiaodong

doi:10.1080/09168451.2020.1807901

Cited by 4 publications

(2 citation statements)

References 38 publications

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“…Moreover, it provides a wide range of gene sets and their data on various stages of normal wound healing ( 6 ). These types of time-course clinical trials make the opportunity for researchers to examine the dynamic behavior of gene expression and the differences that may be available in molecular and cellular status over time ( 7 ). Some recent studies have studied the role of DNA microarrays in physiological and pathophysiological transcriptional responses during the wound-healing process ( 8 ).…”

Section: Introductionmentioning

confidence: 99%

A Review of Genes Involved in Wound Healing

Farhangniya,

Samadikuchaksaraei

2023

MJIRI

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Background Gene therapy holds immense potential in the field of wound healing. However, we still do not recognize this procedure well enough to give oversight effectively to improve healing processes. A wide range of information has been achieved from the database for gene expression profiling by clinical trials, So we performed this study to gain a better understanding of the mechanisms behind wound healing and how it could be utilized to develop new therapies and treatments. Methods In this study, we have been focusing on wound-healing genes, conducting a thorough review to explore the various genes and pathways involved in this process. For this purpose, a total of 320 articles were collected. All experimental studies, systematic or narrative reviews, studies and clinical trials included in this paper were searched on PubMed, Medline, Embase, Science Direct, and Scopus databases in English using the following terms: Wound Healing, wound regeneration, Gene Transfer, and Gene Therapy were used to search the mentioned databases. Unfortunately, we didn’t find a large sample cohort study on this topic. A total amount of 330 articles were collected based on the guidelines of the PRISMA method. Both inclusion and exclusion criteria were settled. Results During the last decade, different models of gene delivery have been introduced, which include viral transfection and Non-viral techniques. In this regard, TIMP-2 protein and VEGF mutants such as VEGF165, CARP, and HIF-1 are the genes that accelerate the rate of tissue repair. Conclusion The process of wound healing is mainly related to the change of expression of genes that have a role in the parts of inflammation and repair. In our study, some of the most suitable genes involved in the wound-healing process are mentioned.

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Section: Introductionmentioning

confidence: 99%

A Review of Genes Involved in Wound Healing

Farhangniya,

Samadikuchaksaraei

2023

MJIRI

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“…MicroRNA-344 (miR-344) was isolated initially from embryonic primary rat cortical neurons [ 16 ]. It is expressed during mouse brain development [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

Significant role of some miRNAs as biomarkers for the degree of obesity

Gouda,

Ahmed,

Mageed

et al. 2023

Journal of Genetic Engineering and Biotechnology

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Background Obesity is one of the most serious problems over the world. MicroRNAs have developed as main mediators of metabolic processes, playing significant roles in physiological processes. Thus, the present study aimed to evaluate the expressions of (miR-15a, miR-Let7, miR-344, and miR-365) and its relationship with the different classes in obese patients. Methods A total of 125 individuals were enrolled in the study and classified into four groups: healthy non-obese controls (n = 50), obese class I (n = 24), obese class II (n = 17), and obese class III (n = 34) concerning body mass index (BMI < 30 kg/m2, BMI 30–34.9 kg/m2, BMI 35–39.9 kg/m2 and BMI ≥ 40 kg/m2, respectively). BMI and the biochemical measurements (fasting glucose, total cholesterol, triglycerides, HDL and LDL, urea, creatinine, AST, and ALT) were determined. The expressions of (miR-15a, miR-Let7, miR-344, and miR-365) were detected through quantitative real-time PCR (RT-qPCR). Results There was a significant difference between different obese classes and controls (P < 0.05) concerning (BMI, TC, TG, HDL, and LDL). In contrast, fasting glucose, kidney, and liver functions had no significant difference. Our data revealed that the expression of miR-15a and miR-365 were significantly associated with different obese classes. But the circulating miR-Let7 and miR-344 were not significantly related to obesity in different classes. Conclusion Our study indicated that miR-15a and miR-365 might consider as biomarkers for the obesity development into different obese classes. Thus, the relationship between regulatory microRNAs and disease has been the object of intense investigation.

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miR-31, miR-155, and miR-221 Expression Profiles and Their Association With Graft Skin Tolerance in a Syngeneic vs Allogeneic Murine Skin Transplantation Model

Mandujano-Tinoco

González-García

Salgado

et al. 2022

Journal of Burn Care &Amp; Research

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Grafting is the gold standard for the treatment of severe skin burns. Frequently, allogeneic tissue is the only transient option for wound coverage, but their use risks damage to surrounding tissues. MicroRNAs have been associated with acute rejection of different tissues/organs. In this study, we analyzed the expression of miR-31, miR-155, and miR-221 and associate it with graft tolerance or rejection using a murine full-thickness skin transplantation model. Recipient animals for the syngeneic and allogeneic groups were BALB/c and C57BL/6 mice, respectively; donor tissues were obtained from BALB/c mice. After 7 days post-transplantation (DPT), the recipient skin and grafts in the syngeneic group maintained most of their structural characteristics and transforming growth factor (TGF)β1 and TGFβ3 expression. Allografts were rejected early (Banff grades II and IV at 3 and 7 DPT, respectively), showing damage to the skin architecture and alteration of TGFβ3 distribution. miRNAs skin expression changed in both mouse strains; miR-31 expression increased in the recipient skin of syngeneic grafts relative to that of allogeneic grafts at 3 and 7 DPT (p < 0.05 and p < 0.01, respectively); miR-221 expression increased in the same grafts at 7 DPT (p < 0.05). The only significant difference between donor tissues was observed for miR-155 expression at 7 DPT which was associated with necrotic tissue. Only miR-31 and miR-221 levels were increased in the blood of BALB/c mice that received syngeneic grafts after 7 DPT. Our data suggest that local and systemic miR-31 and miR-221 overexpression are associated with graft tolerance.

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Analysis of the differential expression profile of miRNAs in myocardial tissues of rats with burn injury

Cited by 4 publications

References 38 publications

A Review of Genes Involved in Wound Healing

A Review of Genes Involved in Wound Healing

Significant role of some miRNAs as biomarkers for the degree of obesity

miR-31, miR-155, and miR-221 Expression Profiles and Their Association With Graft Skin Tolerance in a Syngeneic vs Allogeneic Murine Skin Transplantation Model

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