Analysis of the effect of smoking on the buccal microbiome using next-generation sequencing technology

Karabudak, Sema; Arı, Oğuz; Durmaz, Bengül; Dal, Tuba; Başyiğit, Tuğcan; Kalcıoğlu, M. Tayyar; Durmaz, Rıza

doi:10.1099/jmm.0.001003

Cited by 30 publications

(32 citation statements)

References 35 publications

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“…Megasphaera micronuciformis were reported to be found more commonly in cigarette smoker's upper gastrointestinal tract, as detected using the microarray 18 , and they are under the family Veillonellaceae. The genus Rothia is an oral commensal, and Rothia mucilaginosa was recently reported to be increased in current smoker's buccal microbiome 19 , although our analysis in tongue microbiome did not find significant difference in terms of relative abundance. We performed strain sharing pattern analysis to further elucidate the meaning of these differences, and two Veillonella species have relatively high strain sharing within groups compared to between groups.…”

Section: Species_idcontrasting

confidence: 86%

Metagenomic analysis of bacterial species in tongue microbiome of current and never smokers

Sato

Kakuta

Hasegawa

et al. 2020

npj Biofilms Microbiomes

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Cigarette smoking affects the oral microbiome, which is related to various systemic diseases. While studies that investigated the relationship between smoking and the oral microbiome by 16S rRNA amplicon sequencing have been performed, investigations involving metagenomic sequences are rare. We investigated the bacterial species composition in the tongue microbiome, as well as single-nucleotide variant (SNV) profiles and gene content of these species, in never and current smokers by utilizing metagenomic sequences. Among 234 never smokers and 52 current smokers, beta diversity, as assessed by weighted UniFrac measure, differed between never and current smokers (pseudo-F = 8.44, R 2 = 0.028, p = 0.001). Among the 26 species that had sufficient coverage, the SNV profiles of Actinomyces graevenitzii, Megasphaera micronuciformis, Rothia mucilaginosa, Veillonella dispar, and one Veillonella sp. were significantly different between never and current smokers. Analysis of gene and pathway content revealed that genes related to the lipopolysaccharide biosynthesis pathway in Veillonella dispar were present more frequently in current smokers. We found that species-level tongue microbiome differed between never and current smokers, and 5 species from never and current smokers likely harbor different strains, as suggested by the difference in SNV frequency.

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Section: Species_idcontrasting

confidence: 86%

Metagenomic analysis of bacterial species in tongue microbiome of current and never smokers

Sato

Kakuta

Hasegawa

et al. 2020

npj Biofilms Microbiomes

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“…Smoking is a wellrecognized risk factor for HS (Akdogan et al, 2018;Revuz et al, 2008), but the link between smoking and HS is poorly understood. Cigarette smoking has been shown to alter oral and gut microbiomes (Karabudak et al, 2019;Savin et al, 2018;Wu et al, 2016). Our study, as well as previous reports, did not identify smoking as an independent factor associated with skin dysbiosis in HS (Ring et al, 2017;Schneider et al, 2020).…”

Section: Hs Samples Controlssupporting

confidence: 54%

The Surface Microbiome of Clinically Unaffected Skinfolds in Hidradenitis Suppurativa: A Cross-Sectional Culture-Based and 16S rRNA Gene Amplicon Sequencing Study in 60 Patients

Riverain-Gillet¹,

Guet-Revillet

Jaı̈s

et al. 2020

Journal of Investigative Dermatology

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Hidradenitis suppurativa (HS) is a chronic inflammatory disease of the skin associated with specific lesional dysbiotic features. We studied the microbiome of clinically unaffected typical HS sites (armpits, inguinal folds, and gluteal clefts) in 60 patients with HS and 17 healthy controls. A total of 192 samples obtained by swabbing were analyzed by bacterial cultures. Of these, 116 randomly selected samples were studied by 16S rRNA gene amplicon sequencing. Patients and controls showed similar characteristics, except for smoking (87% vs. 6%, respectively). HS skinfolds were characterized by an increased abundance of anaerobes, predominantly Prevotella, but also Actinomyces, Campylobacter ureolyticus, and Mobiluncus, contrasting with a lower abundance of skin commensals such as Staphylococcus epidermidis, a major component of the skin microbiome; Kocuria; and Micrococcus luteus. The following three independent factors were associated with an abundance of high anaerobes by multivariate analysis: samples originating from patients with HS patients (P ¼ 2.1 Â 10 À4); body mass index (P ¼ 5 Â 10 À5); and the sampling site, the gluteal cleft being the most anaerobic area, followed by inguinal folds and axilla (P ¼ 3 Â 10 À6). The microbiome of clinically unaffected HS skinfolds is reminiscent, albeit to a minor extent, of the microbiome of chronic suppurative HS lesions and may fuel inflammation at a preclinical stage of the disease.

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“…Neither studies found the relative abundance of Rothia to be associated with smoking, but the metagenomic study revealed R. mucilaginosa found in the current smokes to have significantly less gene variations (or strains) compared to the never smokers. However, another study on the buccal microbiome found this species to be enriched by smoking [31].…”

Section: Discussionmentioning

confidence: 96%

Tongue microbiome of smokeless tobacco users

et al. 2020

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Background: The possibility that smokeless tobacco may contribute to oral carcinogenesis by influencing the oral microbiome has not been explored. This preliminary cross-sectional study sought to assess the effect of using shammah, a form of smokeless tobacco prevalent in Arabia, on the tongue microbiome. Tongue scarping samples were obtained from 29 shammah users (SU; 27.34 ± 6.9 years) and 23 shammah non-users (SNU; 27.7 ± 7.19 years) and analyzed with 16S rRNA gene sequencing (V1-V3). Species-level taxonomy assignment of the high-quality, merged reads was obtained using a previously described BLASTn-based algorithm. Downstream analyses were performed with QIIME, LEfSe, and R. Results: A total of 178 species, belonging to 62 genera and 8 phyla were identified. Genera Streptococcus, Leptotrichia, Actinomyces, Veillonella, Haemophilus, Prevotella and Neisseria accounted for more than 60% of the average microbiome. There were no differences between the two groups in species richness and alpha-diversity, but PCoA showed significant separation (P = 0.015, ANOSIM). LEfSe analysis identified 22 species to be differentially abundant between the SU and SNU. However, only 7 species maintained a false discovery rate of ≤0.2 and could cluster the two groups separately: Rothia mucilaginosa, Streptococcus sp. oral taxon 66, Actinomyces meyeri, Streptococcus vestibularis Streptococcus sanguinis and a potentially novel Veillonella species in association with SU, and Oribacterium asaccharolyticum with SNU. Conclusion: These preliminary results indicate that shammah use induces tongue microbiome changes including enrichment of several species with high acetaldehyde production potential, which warrants further investigation.

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Analysis of the effect of smoking on the buccal microbiome using next-generation sequencing technology

Cited by 30 publications

References 35 publications

Metagenomic analysis of bacterial species in tongue microbiome of current and never smokers

Metagenomic analysis of bacterial species in tongue microbiome of current and never smokers

The Surface Microbiome of Clinically Unaffected Skinfolds in Hidradenitis Suppurativa: A Cross-Sectional Culture-Based and 16S rRNA Gene Amplicon Sequencing Study in 60 Patients

Tongue microbiome of smokeless tobacco users

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