Analysis of the function of KIF3A and KIF3B in the spermatogenesis in Boleophthalmus pectinirostris

Abstract

Spermatogenesis represents one of the most complicated morphological transformation procedures. During this process, the assembly and maintenance of the flagella and intracellular transport of membrane-bound organelles required KIF3A and KIF3B. Our main goal was to test KIF3A and KIF3B location during spermatogenesis of Boleophthalmus pectinirostris. We cloned complete cDNA of KIF3A/3B from the testis of B. pectinirostris by PCR and rapid amplification of cDNA ends (RACE). The predicted secondary and tertiary structures of B. pectinirostris KIF3A/3B contained three domains: (a) the head region, (b) the stalk region, and (c) the tail region. Real-time quantitative PCR (qPCR) results revealed that KIF3A and KIF3B mRNA were presented in all the tissues examined, with the highest expression seen in the testis. In situ hybridization (ISH) showed that KIF3A and KIF3B were distributed in the periphery of the nuclear in the spermatocyte and the early spermatid. In the late spermatid and mature sperm, the KIF3A and KIF3B mRNA were gradually gathered to one side where the flagella formed. Immunofluorescence (IF) showed that KIF3A, tubulin, and mitochondria were co-localized in different stages during spermiogenesis in B. pectinirostris. The temporal and spatial expression dynamics of KIF3A/3B indicate that KIF3A and KIF3B might be involved in flagellar assembly and maintenance at the mRNA and protein levels. Moreover, these proteins may transport the mitochondria resulting in flagellum formation in B. pectinirostris.

Identification of kinesin family member 3B (KIF3B) as a molecular target for gastric cancer

“…26 Additionally, KIF3B is a key regulator in multiple cellular processes including mitosis, spermatogenesis, and axonal regeneration. 12,27 These cellular processes are all involved in the motor protein function of KIF3B. In this study, we found and confirmed the effect of KIF3B on the proliferation of GC cells, which is probably also due to the effect on microtubule-based material transport to achieve the regulation of cell proliferation.…”

“…KIF3B has also been reported to be involved in the transport of membrane‐bound complexes in neurons and epithelial cells . Additionally, KIF3B is a key regulator in multiple cellular processes including mitosis, spermatogenesis, and axonal regeneration . These cellular processes are all involved in the motor protein function of KIF3B.…”

“…Kinesin family member 3B (KIF3B) is known as a microtubule motor kinesin and one of the most ubiquitously expressed KIFs . In decades, studies showed that KIF3B is related to lots of physiological responses, such as mitosis, spermatogenesis, ciliary assembly, and vasculotropism . Previous study also declared that KIF3B phosphorylation‐mediated kinesin‐II interaction with intraflagellar transport protein‐B (IFT‐B) and therefore contributed to IFT entry and turnaround .…”

Identification of bi-allelic KIF9 loss-of-function variants contributing to asthenospermia and male infertility in two Chinese families

“…Another member of the kinesin superfamily, KIF3B, has also been found to be mutated in some cases of male infertility ( 33 ). The function of KIF3B has been studied in the context of intracellular transport and spermatogenesis in many species ( 34 – 36 ). A human A>T variant in OAT patients was linked to decreased KIF3B expression resulting in impaired male fertility ( 33 ).…”

The complete mitochondrial genome of the mudskipper, Boleophthalmus pectinirostris (Gobiiformes, Oxudercidae) from Beibu Bay

“…The mudskipper, Boleophthalmus pectinirostris (Gobiiformes, Oxudercidae) is an amphibious gobioid teleosts, which is a commercially important aquaculture species (Zhao et al 2018). It is a warm water fish that distributes in tropical mudflats along the coast of Japan, Taiwan and Southern China (Clayton 1993;Liu et al 2012).…”