Analysis of the genetic structure of the European eel Anguilla anguilla using the mtDNA D-loop region molecular marker

Ragauskas, Adomas; Butkauskas, Dalius; Sruoga, Aniolas; Kesminas, Vytautas; Rashal, Isaak; Tzeng, Wann‐Nian

doi:10.1007/s12562-014-0714-1

Cited by 10 publications

(18 citation statements)

References 57 publications

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“…The base composition is 32.1%, 33.3%, 19.2%, and 15.5% for A, T, C, and G, respectively. Like that of other vertebrates (Guo et al 2003;Ragauskas et al 2014;Wang et al 2014;Gong et al 2015), the CR of this species is also partitioned into three domains and some similar conserved-sequence blocks are found, including the termination associated sequence (TAS), the central conserved sequence blocks (CSB-F, D, C and A) and the conserved sequence blocks (CSB-2 and 3).…”

mentioning

confidence: 90%

Characterization of the complete mitochondrial genome of Wuhaniligobius polylepis (Gobiiformes: Gobiidae) and phylogenetic studies of Gobiiformes

Gong

Liu

Lü

et al. 2018

Mitochondrial DNA Part B

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The environmental changes mainly caused by water pollution and human activities have dramatically threatened the survival of the small-scaled Wu's goby W. polylepis. In the present study, the complete mitogenome of Wuhaniligobius polylepis was determined, which is 16,496 bp in length, containing 13 PCGs, two rRNA genes, 22 tRNA genes and a putative control region. The overall base composition is 28.6%, 29.0%, 26.7%, and 15.7% for A, T, C and G, respectively. The 13 PCGs encode 3,802 amino acids in total, twelve of which use the initiation codon ATG except COI, which uses GTG. In order to explore the systematic status of W. polylepis and further phylogenetic study of Gobiiformes, a maximumlikelihood tree was constructed based on the concatenated sequences of 12 PCGs. The result showed that Pseudogobius, Hemigobius, and Mugilogobius clustered into a clade and then formed a sister-group with W. polylepis, suggesting the invalid former name of M. polylepis or E. polylepis.

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confidence: 90%

Characterization of the complete mitochondrial genome of Wuhaniligobius polylepis (Gobiiformes: Gobiidae) and phylogenetic studies of Gobiiformes

Gong

Liu

Lü

et al. 2018

Mitochondrial DNA Part B

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“…The first attempts of DNA sequencing of mtDNA D-loop (Lintas et al, 1998) or cyt b (Daemen et al, 2001) regions were not enough to show the actual potential of these molecular markers (Van Ginneken and Maes, 2005). New information and insights about the population genetic structure and intraspecific evolution of the European eel were obtained by two recently conducted, independent studies, which used the mtDNA D-loop region (Ragauskas et al, 2014) and the ND5 region (Baltazar-Soares et al, 2014) sequence analysis.…”

Section: Introductionmentioning

confidence: 99%

“…These papers re-opened eel research using direct sequencing of different mtDNA regions and raised new important questions concerning the population genetic structure of this species (Baltazar-Soares and Eizaguirre, 2016). Daemen et al (2001) suggested that the population genetic structure of the European eel occurs in a genetic mosaic consisting of isolated groups, but only after the studies of Baltazar-Soares et al 2014, Ragauskas et al (2014) and Baltazar-Soares and Eizaguirre (2016) the existence of reproductively isolated groups that are represented by phylogenetically related individuals has been re-evaluated. The existence of a genetic mosaic in the population of the European eel could be explained by a cryptic philopatric behavior of females spawning in distinct areas in the spawning site.…”

Section: Introductionmentioning

confidence: 99%

“…This cryptic organization may contribute to the maintenance of the adaptive potential of the species (Baltazar-Soares and Eizaguirre, 2016). Despite the fact that significant genetic differentiation was not detected using the mtDNA Dloop region (Ragauskas et al, 2014), a strong and significant genetic structure was observed using the ND5 region (Baltazar-Soares et al, 2014). Therefore, it seems that the ND5 region is more sensitive to the natural selection.…”

Section: Introductionmentioning

confidence: 99%

“…In order to get more valuable information about the population genetic structure of the European eel additional mtDNA genetic markers should be used. It is reasonable to select cyt b as type I molecular marker, i.e., a marker associated with a gene of known function (Liu and Cordes, 2004), to complement the mtDNA D-loop region data, which were recently used for the evaluation of the population genetic structure of the European eel (Ragauskas et al, 2014). Thus far the analysis of mtDNA cyt b sequences has revealed no clear population genetic structure based on the distribution of different haplotypes of 107 individuals (Daemen et al, 2001) and no more attempts have been made to carry out similar studies into this species on the basis of the utilization of this genetic marker.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Distinct matrilines in the panmictic population of the European eelAnguilla anguilla?

Ragauskas

Butkauskas

Bianchini

2017

Aquat. Living Resour.

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Determining the genetic population structure and intraspecific evolution of species is important for efficient management. Here we investigated 394 sequences of the mtDNA cyt b of the critically endangered European eel, Anguilla anguilla. We found 62 different haplotypes, among which 32 novel ones. All those sequences were grouped into 9 haplogroups on the basis of their frequency and links in the haplotype network. Two pairwise comparisons revealed significant genetic differentiation between regions. One comparison indicated small (F ST = 0.02768; P = 0.0071) genetic differentiation between the Atlantic Ocean and the North Sea. Another comparison indicated small (F ST = 0.01144; P = 0.0440) genetic differentiation between eel samples caught in the Baltic Sea, the Curonian Lagoon and the Gulf of Riga and those captured in the North Sea. The results of the study are in agreement with the recent findings obtained using the mtDNA D-loop region as a molecular marker, indicating that although A. anguilla is considered a panmictic species the genetic structure of its population indicated by mtDNA markers could be characterized as a genetic mosaic.

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Estimating number of European eel (Anguilla anguilla) individuals using environmental DNA and haplotype count in small rivers

Halvorsen

Korslund

Mattingsdal

et al. 2023

Ecology and Evolution

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Knowledge about population genetic data is important for effective conservation management. Genetic research traditionally requires sampling directly from the organism, for example tissue, which can be challenging, time‐consuming, and harmful to the animal. Environmental DNA (eDNA) approaches offer a way to sample genetic material noninvasively. In attempts to estimate population size of aquatic species using eDNA, researchers have found positive correlations between biomass and eDNA concentrations, but the approach is debated because of variations in the production and degrading of DNA in water. Recently, a more accurate eDNA‐approach has emerged, focusing on the genomic differences between individuals. In this study, we used eDNA from water samples to estimate the number of European eel (Anguilla anguilla) individuals by examining haplotypes in the mitochondrial D‐loop region, both in a closed aquatic environment with 10 eels of known haplotypes and in three rivers. The results revealed that it was possible to find every eel haplotype in the eDNA sample collected from the closed environment. We also found 13 unique haplotypes in the eDNA samples from the three rivers, which probably represent 13 eel individuals. This means that it is possible to obtain genomic information from European eel eDNA in water; however, more research is needed to develop the approach into a possible future tool for population quantification.

show abstract

Analysis of the genetic structure of the European eel Anguilla anguilla using the mtDNA D-loop region molecular marker

Cited by 10 publications

References 57 publications

Characterization of the complete mitochondrial genome of Wuhaniligobius polylepis (Gobiiformes: Gobiidae) and phylogenetic studies of Gobiiformes

Characterization of the complete mitochondrial genome of Wuhaniligobius polylepis (Gobiiformes: Gobiidae) and phylogenetic studies of Gobiiformes

Distinct matrilines in the panmictic population of the European eelAnguilla anguilla?

Estimating number of European eel (Anguilla anguilla) individuals using environmental DNA and haplotype count in small rivers

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