Analysis of the hematopoietic potential of muscle-derived cells in mice

Geiger, Hartmut; True, Jarrod M.; Grimes, Barry; Carroll, Elizabeth J.; Fleischman, Roger A.; Zant, Gary Van

doi:10.1182/blood.v100.2.721

Cited by 44 publications

(32 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This explains, for example, why heterogeneous populations of stem cells could also be detected in various organs (eg HSC in muscle tissue). [59][60][61][62] …”

Section: Evidence That Tissue Damage Triggers Circulation Of Tcscmentioning

confidence: 99%

Bone marrow as a home of heterogenous populations of nonhematopoietic stem cells

et al. 2005

View full text Add to dashboard Cite

Evidence is presented that bone marrow (BM) in addition to CD45 positive hematopoietic stem cells contains a rare population of heterogenous CD45 negative nonhematopoietic tissue committed stem cells (TCSC). These nonhematopoietic TCSC (i) are enriched in population of CXCR4 þ CD34 þ AC133 þ lin À CD45 À and CXCR4 þ Sca-1 þ lin À CD45 À in humans and mice, respectively, (ii) display several markers of pluripotent stem cells (PSC) and (iii) as we envision are deposited in BM early in development. Thus, since BM contains versatile nonhematopoietic stem cells, previous studies on plasticity trans-dedifferentiation of BM-derived hematopoietic stem cells (HSC) that did not include proper controls to exclude this possibility could lead to wrong interpretations. Therefore, in this spotlight review we present this alternative explanation of 'plasticity' of BMderived stem cells based on the assumption that BM stem cells are heterogenous. We also discuss a potential relationship of TCSC/PSC identified by us with other BM-derived CD45 negative nonhematopoietic stem cells that were recently identified by other investigators (eg MSC, MAPC, USSC and MIAMI cells). Finally, we discuss perspectives and pitfalls in potential application of these cells in regenerative medicine.

show abstract

“…This explains, for example, why heterogeneous populations of stem cells could also be detected in various organs (eg HSC in muscle tissue). [59][60][61][62] …”

Section: Evidence That Tissue Damage Triggers Circulation Of Tcscmentioning

confidence: 99%

Bone marrow as a home of heterogenous populations of nonhematopoietic stem cells

et al. 2005

View full text Add to dashboard Cite

show abstract

“…However, later studies demonstrated that muscle-derived cells with hematopoietic activity were resident HSCs and not muscle cells that had transdifferentiated in response to hematopoietic signals (McKinney-Freeman et al, 2002;Geiger et al, 2002;Issarachai et al, 2002). Nevertheless, if it were possible to maintain and expand human HSCs from muscle, muscle biopsies could provide an alternative source of HSCs for clinical transplantation.…”

Section: Evidence For and Against Lineage Promiscuity By Adult Stem Cmentioning

confidence: 99%

Untitled

2003

Biology of the Cell

View full text Add to dashboard Cite

Stem cells are believed to be important for regeneration of several adult tissues. Recently, adult stem cells with very broad differentiation potential have been identified although whether they represent vestigial primitive pluripotent stem cells or products of extremely rare de-differentiation events involving tissue-specific stem cells is not known. Transdifferentiation of tissue-specific stem cells across lineage boundaries has also been demonstrated but the relative inefficiency of the process in vivo, even in the presence of tissue damage, questions whether such a mechanism is of physiologic relevance. Interestingly, among adult stem cells, the capacity for lineage switching appears to be greatest in stem cells that can be cultured ex vivo for extended periods. If the normal cell fate decisions of diverse adult stem cell types could be reliably redirected at high frequency in situ, possible regenerative therapies for a wide variety of diseases could be envisioned. An integrated understanding of the transcriptional regulatory networks that comprise the various adult stem cell entities as well as the signaling pathways governing their differentiation into therapeutically useful cell types will facilitate clinical application of these exciting findings.

show abstract

“…These cells have been shown to give rise to long-term hematopoiesis [2], and their ability to generate hematopoietic cells in at least three generations of lethally irradiated recipients for more than 1 year suggests a selfrenewal ability [3]. The contamination of muscle tissue by a circulating stem cell has been suggested as a possible explanation for this phenomenon [5], but the transplantation of many nucleated blood cells did not yield any survival in irradiated mice [5,8,9]. The current hypothesis is that there is a hematopoietic cell niche in the muscle tissue that is either of post-natal or embryonic origin.…”

Section: Introductionmentioning

confidence: 99%

Quantitative and qualitative in vitro analysis of the stem cell potential of hematopoietic cells purified from murine skeletal muscle

et al. 2007

View full text Add to dashboard Cite

The murine skeletal muscle contains hematopoietic stem cells, but this potential has so far not been studied quantitatively or qualitatively in vitro. To quantify the hematopoietic stem cell potential, we have used highly purified SP/CD45 + cells in long-term culture initiating cell (LTC-IC) assays. The SP/CD45+ cell population purified from murine muscle was found to have significant stem cell activity with an LTC-IC frequency of 1/640. Single-cell-sorted SP/CD45 + cells from muscle exhibited robust proliferative activity in vitro at day 16 (380-fold amplification), especially after culture with OP-9 layers that also support embryonic stem cells. Amplified cell populations originating from single cells exhibited multilineage differentiation ability with evidence of myeloid, lymphoid and NK cell markers. Thus, our results demonstrate that hematopoietic stem cells that can be quantified by LTC-IC assays exist in the murine skeletal muscle and show also for the first time, at the single-cell level, that these cells exhibit multilineage differentiation ability and major proliferative potential.

show abstract

Analysis of the hematopoietic potential of muscle-derived cells in mice

Cited by 44 publications

References 12 publications

Bone marrow as a home of heterogenous populations of nonhematopoietic stem cells

Bone marrow as a home of heterogenous populations of nonhematopoietic stem cells

Untitled

Quantitative and qualitative in vitro analysis of the stem cell potential of hematopoietic cells purified from murine skeletal muscle

Contact Info

Product

Resources

About