Analysis of the lipid composition of human and boar spermatozoa by MALDI-TOF mass spectrometry, thin layer chromatography and 31P NMR spectroscopy

Lessig, Jacqueline; Gey, Claudia; Süß, Rosemarie; Schiller, Jürgen; Glander, H.‐J.; Arnhold, Jürgen

doi:10.1016/j.cbpc.2003.12.001

Cited by 69 publications

(60 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This decrease in lipid saturation has been suggested to increase membrane fluidity, affect rafts, and provide protection against oxidation (36). One PUFA, 22:6n3 (docosahexaenoic acid or DHA), is prevalent in sperm (16,36) and has been found to decrease the amount of diacylglycerol (DAG) in rafts, possibly induce raft SM degradation to ceramide, and increase apoptosis in cancer cells (30).…”

Section: Lipid Signaling Related To Fertilizationmentioning

confidence: 99%

“…Sperm, melanocytes, and nerve cells have high levels of PUFA (16,36). The Xenopus egg averages ?40 mol% PUFA in the phospholipids CL, PC, PE, and PI/PS, whereas there is only ?13 mol% in LPC and SM.…”

Section: Membrane Phospholipid Saturation and Unsaturationmentioning

confidence: 99%

“…The PUFA 22:6n3 (DHA) has been suggested to be a major regulator of membrane fluidity and raft structure, whereas high levels offer oxidation protection and can lead to apoptosis (16,30,36). (16).…”

Section: Membrane Phospholipid Saturation and Unsaturationmentioning

confidence: 99%

See 2 more Smart Citations

Lipid levels in sperm, eggs, and during fertilization in Xenopus laevis

Petcoff

Holland

Stith

2008

Journal of Lipid Research

View full text Add to dashboard Cite

Critical developmental periods, such as fertilization, involve metabolic activation, membrane fusion events such as sperm-egg or plasma membrane-cortical granule merger, and production and hydrolysis of phospholipids. However, there has been no large-scale quantification of phospholipid changes during fertilization. Using an enzymatic assay, traditional FA analysis by TLC and gas chromatography, along with a new method of phospholipid measurement involving HPLC separation and evaporative light-scattering detection, we report lipid levels in eggs, sperm, and during fertilization in Xenopus laevis. Sperm were found to contain different amounts of phospholipids as compared with eggs. During fertilization, total phosphatidylinositol, lysophosphatidylcholine, sphingomyelin, and phosphatidylserine decreased, and ceramide increased, whereas there was no change in phosphatidylcholine, cardiolipin, or phosphatidylethanolamine. FA analysis of phospholipids found numerous changes during fertilization. Because there is an increase in sn-1,2-diacylglycerol at fertilization, the FAs associated with this increase and the source of the increase in this neutral lipid were examined. Finally, activation of phospholipase C, phospholipase D, phospholipase A2, autotoxin, and sphingomyelinase at fertilization is discussed. Major goals of the LIPID Metabolites and Pathways Strategy (LIPID MAPS) are to "separate and detect all of the lipids in a specific cell," "to quantitate each of the lipid metabolites present and determine the changes in their levels," and to study lipid metabolic pathways during biological events (1). Characterizing changes in the lipid composition during defined biological events is part of the concept of "functional lipidomics" (2). These fundamental, descriptive studies will facilitate our understanding of how lipids regulate membrane fluidity and fusion, lipid rafts, the cytoskeleton, kinases, and protein binding to membranes (e.g., 3-5) to induce pleiotropic cellular effects.About 15 years ago, quantification of lipid mass changes during maturation of the oocyte to the fertilizable egg, and during fertilization of the egg by sperm, was begun in Xenopus laevis (6-9). Critical developmental events can be isolated in vitro with this model system, and large numbers of gametes can be obtained to provide sufficient amounts of second messengers and lipids. During fertilization, membrane bending and fusion are associated with the acrosome reaction of sperm (wherein the large sperm acrosome undergoes exocytosis upon interaction with layers that cover the egg), with sperm-egg merger, and with subsequent fertilization events such as cortical granule exocytosis (10, 11). Furthermore, both Xenopus and human eggs are arrested in meiosis II (12).Compared with oocytes, one disadvantage of studying eggs is that they have a short life, much less than the estimated time for lipid precursor incorporation to near equilibrium levels (6, 7). Thus, lipid analysis based on label incorporation or turnover is not reliable, and ...

show abstract

Section: Lipid Signaling Related To Fertilizationmentioning

confidence: 99%

Section: Membrane Phospholipid Saturation and Unsaturationmentioning

confidence: 99%

See 1 more Smart Citation

Lipid levels in sperm, eggs, and during fertilization in Xenopus laevis

Petcoff

Holland

Stith

2008

Journal of Lipid Research

View full text Add to dashboard Cite

show abstract

“…The most commonly used matrix is 2,5-dihydroxybenzoic acid (DHB). DHB is an effective matrix in the positive ion mode, but its acidic nature does not work well in the negative ion mode as it produces an intense background Lessig et al, 2004). Another matrix becoming more common is p-nitroaniline (PNA).…”

Section: Soft Ionization Methodsmentioning

confidence: 99%

“…Since PC has such a strong intensity, it often overshadows the lower response of PE. For this reason, it may be necessary to first separate the classes using HPLC methods Lessig et al, 2004;Sowa and Subbaiah, 2004), as discussed above.…”

Section: Analysis Of Phospholipidsmentioning

confidence: 99%

A review of chromatographic methods for the assessment of phospholipids in biological samples

Peterson

Cummings

2005

Biomedical Chromatography

236

159

View full text Add to dashboard Cite

Phospholipids are important constituents of all living cell membranes. Lipidomics is a rapidly growing field that provides insight as to how specific phospholipids play roles in normal physiological and disease states. There are many analytical methods available for the qualitative and quantitative determination of phospholipids. This review provides a summary of the methods that were historically used such as thin layer chromatography, gas chromatography and high-performance liquid chromatography. In addition, an introduction to applications of interfacing these traditional chromatographic techniques with mass spectrometry is provided.

show abstract