Analysis of the monosaccharide composition of water-soluble polysaccharides from Sargassum fusiforme by high performance liquid chromatography/electrospray ionisation mass spectrometry

Determination of carbohydrates in tobacco by pressurized liquid extraction combined with a novel ultrasound-assisted dispersive liquid–liquid microextraction method

“…Regarding HPLC-MS/MS methods, a drawback of matrix effect was also obvious, which were attributed to the high hydrophilic for carbohydrates. Although reference 8 provided relatively lower LODs, a large number of references showed that direct HPLC-MS/MS method was limited by loss of sensitivity owing to the low ionisation efficiency of carbohydrates [36][37][38].…”

Physicochemical characterization of five types of citrus dietary fibers

“…Monosaccharide composition of SDF was measured according to the method described by Wu et al (2014) with some modifications. Briefly, 0.15 g SDF was mixed with 7 mL sulfuric acid solution (6 mol/L) in a stoppered test tube and stayed at ambient temperature for 1 h for dissolution.…”

Revisiting monosaccharide analysis – quantitation of a comprehensive set of monosaccharides using dynamic multiple reaction monitoring

“…Compounds that coelute such as ribose, allose, and glucuronic acid have different masses and were readily distinguished by MS. With the solvents and gradient optimized in this method , baseline separation of corresponding isomeric structures including two deoxyhexoses (rhamnose and fucose), five hexoses (fructose, mannose, allose, glucose, and galactose), two hexuronic acids (GlcA and GalA), and two N -acetyl-hexosamines (GlcNAc and GalNAc) was readily achieved within a 7.5-minute period during a total run time of 10 minutes (Figure 2). Compared to other monosaccharide quantitation methods 23,24,35 , the separation time in this method is greatly shortened with more compounds included.…”

“…Both the standard mixtures and extracted monosaccharides from fecal samples were subjected to derivatization using PMP. The derivatization conditions optimized by Wu et al 24 were used with some modifications. Briefly, 50 μL of monosaccharide standards or fecal samples were mixed with 200 μL ammonia solution (28.0-30.0%) in water and 200 μL 0.2 M PMP solution in methanol.…”

“…23,24 Rühmann et al 25 coupled UV and electrospray ionization with ion trap detection and achieved faster analysis of saccharide-PMP derivatives. Nonetheless, the sensitivity was not greatly improved.…”