Analysis of the morphometric parameters of pig ear hair follicles

Zambrano, Alexandra; Klein, Anna Lena; Patzelt, Alexa

doi:10.1111/srt.13008

Cited by 6 publications

(4 citation statements)

References 27 publications

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“…A PP filter with a pore size of 70 m was selected as a model membrane because the diameter of hair follicles at the skin surface of pig ear is reportedly 113±43 μm, similar to that of human skin. 22) In addition, the surface free energy and its dispersion components of PP which affect the wettability or adhesion of a formulation to a skin surface, 23) are similar to that of human and pig skin. 24) The permeation of DiI was used as a tracer of vehicle permeation through PP.…”

Section: Biological and Pharmaceutical Bulletin Advance Publicationmentioning

confidence: 99%

Rheological Properties and Composition Affecting the Skin Permeation of a Model of a Hydrophilic Drug in Lecithin Reverse Wormlike Micelles

Miyasaka,

Hashizaki,

Shibasaki

et al. 2024

Biological & Pharmaceutical Bulletin

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We investigated the effect of the rheological properties and composition of lecithin reverse wormlike micelles (LRWs) on the skin permeation of a model of a hydrophilic drug to determine whether LRWs support uniform hydrophilic drug/oil-based formulations and good drug penetrate into skin. Here, we prepared LRWs with D (-)ribose (RI) or glycerol (GL) as polar compounds, liquid paraffin (LP) or isopropyl myristate (IPM) as oils, and 6-carboxyfluorescein (CF) as a model for a hydrophilic drug, and evaluated the rheological properties and skin penetration characteristics of the preparations. The LRWs showed moderate viscosity at 25°C, a typical storage temperature, but decreasing viscosity at 32°C, the surface temperature of human skin, suggesting that the LRWs would penetrate the microstructure of skin (e.g., wrinkles and hair follicles). The highest skin permeability of CF was observed when IPM was used as the oil, suggesting that both the stratum corneum and hair follicle routes are involved in drug permeation. The penetration of CF into hair follicles is influenced not only by the rheology of the formulation but also by the interaction between IPM and sebum in the hair follicles.

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Section: Biological and Pharmaceutical Bulletin Advance Publicationmentioning

confidence: 99%

Rheological Properties and Composition Affecting the Skin Permeation of a Model of a Hydrophilic Drug in Lecithin Reverse Wormlike Micelles

Miyasaka,

Hashizaki,

Shibasaki

et al. 2024

Biological & Pharmaceutical Bulletin

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“…Recently, follicular uptake was also shown for lipid-based nanoparticles on the pig ear model. While the pig ear model is suitable to demonstrate HF penetration due to high similarity, it is a tissue that cannot be cultured over a longer time. The lack of cultivability limits the use of biological efficacy and tissue or immune response.…”

Section: Introductionmentioning

confidence: 99%

A Novel 3D Printed Model of Infected Human Hair Follicles to Demonstrate Targeted Delivery of Nanoantibiotics

Aliyazdi,

Frisch,

Neu

et al. 2024

ACS Biomater. Sci. Eng.

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Hair follicle-penetrating nanoparticles offer a promising avenue for targeted antibiotic delivery, especially in challenging infections like acne inversa or folliculitis decalvans. However, demonstrating their efficacy with existing preclinical models remains difficult. This study presents an innovative approach using a 3D in vitro organ culture system with human hair follicles to investigate the hypothesis that antibiotic nanocarriers may reach bacteria within the follicular cleft more effectively than free drugs. Living human hair follicles were transplanted into a collagen matrix within a 3D printed polymer scaffold to replicate the follicle's microenvironment. Hair growth kinetics over 7 days resembled those of simple floating cultures. In the 3D model, fluorescent nanoparticles exhibited some penetration into the follicle, not observed in floating cultures. Staphylococcus aureus bacteria displayed similar distribution profiles postinfection of follicles. While rifampicin-loaded lipid nanocapsules were as effective as free rifampicin in floating cultures, only nanoencapsulated rifampicin achieved the same reduction of CFU/mL in the 3D model. This underscores the hair follicle microenvironment's critical role in limiting conventional antibiotic treatment efficacy. By mimicking this microenvironment, the 3D model demonstrates the advantage of topically administered nanocarriers for targeted antibiotic therapy against follicular infections.

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“…It was shown that porcine ear skin is a well suitable skin model for penetration studies [22,23]. This becomes superior when it comes to the investigation of follicular and transfollicular penetration since the skin remains fixed on the cartilage.…”

Section: Introductionmentioning

confidence: 99%

Microdialysis on Ex Vivo Porcine Ear Skin Can Validly Study Dermal Penetration including the Fraction of Transfollicular Penetration—Demonstrated on Caffeine Nanocrystals

et al. 2021

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Common ex vivo methods for penetration investigations often fail to monitor transfollicular penetration appropriately. In the present investigation, the validity of dermal microdialysis on the ex vivo porcine ear skin to investigate penetration kinetics, including transfollicular penetration, was studied. In setup A, a caffeine nanocrystal formulation was compared to a non-particular caffeine gel formulation. In setup B, two caffeine nanocrystal formulations of different sizes (200 nm, 700 nm) were compared to each other. Microdialysis samples were collected for 46 h. After sampling, the skin layers were separated, homogenized, and caffeine was quantified in all samples. In setup A the area under the curve (AUC) after crystal gel formulation application was 12 times higher than after non-particular formulation application. Setup B showed an increased AUC of 42% in the microdialysis data when the 700 nm caffeine crystals were applied compared to the 200 nm crystals. The microdialysis data was supported by the separation, homogenization and extraction data. Microdialysis performed on ex vivo porcine ear skin is a novel experimental setup. It is of high interest for further investigations since it is able to also capture the impact of follicular and transfollicular penetration kinetics as no other ex vivo setup can.

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Analysis of the morphometric parameters of pig ear hair follicles

Cited by 6 publications

References 27 publications

Rheological Properties and Composition Affecting the Skin Permeation of a Model of a Hydrophilic Drug in Lecithin Reverse Wormlike Micelles

Rheological Properties and Composition Affecting the Skin Permeation of a Model of a Hydrophilic Drug in Lecithin Reverse Wormlike Micelles

A Novel 3D Printed Model of Infected Human Hair Follicles to Demonstrate Targeted Delivery of Nanoantibiotics

Microdialysis on Ex Vivo Porcine Ear Skin Can Validly Study Dermal Penetration including the Fraction of Transfollicular Penetration—Demonstrated on Caffeine Nanocrystals

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