Analysis of the Performance Contribution Factor of a DPF Catalyst State

Tsuji, Makoto; Aono, Norihiko; Murai, Toshimi; Tsuji, S.

doi:10.4271/2005-08-0148

Cited by 1 publication

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Different influx transporters are involved in the transport of essential endogenous nutrients (e.g., amino acids, glucose) from the bloodstream into the CNS and are critical for the normal function of the brain (Tsuji, 2005). Endothelial cell 3D monocultures expressed relatively high levels of SLC2A3 (GLUT3) encoding for glucose transporter-3 (GLUT3) (Figures 6D and 6I), a pump that is more characteristic of all neurons (Simpson et al, 2008), and did not express SLC2A5 (GLUT5) that codes for the transporter GLUT5 (Douard and Ferraris, 2008).…”

Section: Active Influx Transportersmentioning

confidence: 99%

Heterocellular spheroids of the neurovascular blood-brain barrier as a platform for personalized nanoneuromedicine

Kumarasamy

Sosnik

2021

iScience

View full text Add to dashboard Cite

Nanoneuromedicine investigates nanotechnology to target the brain and treat neurological diseases. In this work, we biofabricated heterocellular spheroids comprising human brain microvascular endothelial cells, brain vascular pericytes and astrocytes combined with primary cortical neurons and microglia isolated from neonate rats. The structure and function are characterized by confocal laser scanning and light sheet fluorescence microscopy, electron microscopy, western blotting, and RNA sequencing. The spheroid bulk is formed by neural cells and microglia and the surface by endothelial cells and they upregulate key structural and functional proteins of the blood-brain barrier. These cellular constructs are utilized to preliminary screen the permeability of polymeric, metallic, and ceramic nanoparticles (NPs). Findings reveal that penetration and distribution patterns depend on the NP type and that microglia would play a key role in this pathway, highlighting the promise of this platform to investigate the interaction of different nanomaterials with the central nervous system in nanomedicine, nanosafety and nanotoxicology.

show abstract

Section: Active Influx Transportersmentioning

confidence: 99%