Analysis of the phenolic composition of fungus‐resistant grape varieties cultivated in Italy and Germany using UHPLC‐MS/MS

Abstract

The phenolic composition of grape berries grown in Italy (Cabernet Cortis, Johanniter, Solaris, Phoenix, and Regent) and in Germany (Regent and Phoenix) was analyzed using two complementary LC-MS/MS (Liquid Chromatography - Tandem Quadrupole Mass Spectrometry) methods. These five cultivars belong to fungus-resistant grape varieties (PIWI) and little is known about their phenolic profile, although phenolics such as phytoalexins have been recognized, playing a key role in the resistance mechanism. A triple quadruple mass spectrometer detector was used for both identification and quantification, and analytical tools from untargeted metabolomics were applied to check and control the quality of the results. Specifically, biological, technical, and instrumental replications were included in order to study the variability at different levels. The results allowed tuning of the sampling protocol and provided a rich phenolic metabolite profile for the aforementioned PIWI varieties, as compared with Vitis vinifera cultivars, especially in the class of stilbenes.

Botrytis bunch rot on 'Sauvignon Blanc' grapevine on the Y-trellis and vertical shoot-positioned training systems

“…The average TP content (285.3 mg L -1 ) obtained in the present study is in the expected range for the 'Sauvignon Blanc' variety (Ehrhardt et al, 2014). An increase of 59.5 mg L -1 in TP contents was verified in the 2013/2014 crop season, when botrytis bunch rot was higher in the YT system, and can be related to high plant stress caused by B. cinerea infection.…”

Rapid Characterizaiton of Chemical Constituents of the Tubers of Gymnadenia conopsea by UPLC–Orbitrap–MS/MS Analysis

“…A total of eight stilbenes in the tubers of G. conopsea extract were identified in positive and negative ion modes. According to their molecular mass, formula, MS/MS fragments, and related literature studies, compounds 38, 39, 40, 57, 64, 69, 72, 73, and 76 were considered to be isorhapontigenin [18], rhaponticin [19], piceatannol [20], dihydro-resveratrol [21], batatasin III [22], 3,3′-dihydroxy-4-(4-hydroxybenzyl)-5-methoxybibenzyl [23], bulbocodin C [24], bulbocodin D [24], and 3,3′-dihydroxy-2,6-bis(4-hydroxybenzyl)-5-methoxybibenzyl [25], respectively.…”

Downy mildew intensity in tolerant grapes varieties in highlands of southern Brazil

“…A large number of socalled fungus-resistant PIWI varieties (from the German word 'pilzwiderstandsfähig') therefore emerged in the 20th century [6].…”