Analysis of the potential behavioral impact of methanol when used as a solvent: Dataset from zebrafish (Danio rerio) behavioral research

Hamilton, Trevor J.; Szaszkiewicz, Joshua; Krook, Jeffrey; Burggren, Warren W.

doi:10.1016/j.dib.2021.107018

Cited by 7 publications

(4 citation statements)

References 12 publications

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“…Most previous in vitro and in vivo studies of the effects of LBP used ethanol or methanol extracts in mice or rats, and anxiety-like behavior was rarely assessed [5,[15][16][17][18]. Methanol and ethanol are common vehicles for dissolving drugs administered to experimental organisms, but by themselves, they can alter physiology and behavior at high concentrations [19,20]. Hot aqueous extracts, known as tea, are simple and inexpensive to prepare, making them the most popular form of natural product consumption, being widely consumed by humans, including pregnant women and children, because of their calming and mood-regulating properties [5].…”

Section: Introductionmentioning

confidence: 99%

The psychoactive effects of Bryophyllum pinnatum (Lam.) Oken leaves in young zebrafish

et al. 2022

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Bryophyllum pinnatum (Lam.) Oken (BP) is a plant that is used worldwide to treat inflammation, infections, anxiety, restlessness, and sleep disorders. While it is known that BP leaves are rich in flavonoids, the extent of the beneficial and toxic effects of its crude extracts remains unclear. Although some neurobehavioral studies using leaf extracts have been conducted, none has examined the effects of water-extracted leaf samples. The zebrafish is a powerful animal model used to gain insights into the efficacy and toxicity profiles of this plant due to its high fecundity, external development, and ease of performing behavioral assays. In this study, we performed behavioral testing after acute exposure to different concentrations of aqueous extract from leaves of B. pinnatum (LABP) on larval zebrafish, investigating light/dark preference, thigmotaxis, and locomotor activity parameters under both normal and stressed conditions. LABP demonstrated dose-and time-dependent biphasic effects on larval behavior. Acute exposure (25 min) to 500 mg/L LABP resulted in decreased locomotor activity. Exposure to 300 mg/L LABP during the sleep cycle decreased dark avoidance and thigmotaxis while increasing swimming velocity. After sleep deprivation, the group treated with 100 mg/L LABP showed decreased dark avoidance and increased velocity. After a heating stressor, the 30 mg/L and 300 mg/L LABP-treated groups showed decreased dark avoidance. These results suggest both anxiolytic and psychoactive effects of LABP in a dose-dependent manner in a larval zebrafish model. These findings provide a better understanding of the mechanisms underlying relevant behavioral effects, consequently supporting the safe and effective use of LABP for the treatment of mood disorders.

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Section: Introductionmentioning

confidence: 99%

The psychoactive effects of Bryophyllum pinnatum (Lam.) Oken leaves in young zebrafish

et al. 2022

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“…Shoaling behavior, the inclination of fish to stay in close proximity to their conspecifics, has been extensively studied. 63 The cohesion of a fish shoal may be associated with changes in anxiety-like behavior. Zebrafish locomotion behavior reflects motor coordination, particularly in relation to swimming behavior.…”

Section: ■ Discussionmentioning

confidence: 99%

Insights into Triclosan-Induced Endocrine Disruption: Evidence from the National Health and Nutrition Examination Survey and Zebrafish Models

Li,

Xian,

Ren

et al. 2024

Environ. Health

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Triclosan (TCS) has garnered significant attention due to its widespread use and associated endocrine-disrupting effects. However, its impact on the neuroendocrine system and underlying mechanisms remain poorly understood. Here, we established correlations between TCS exposure and serum sex hormone levels in participants of the National Health and Nutrition Examination Survey (NHANES). Additionally, we investigated TCS's influence on the neuroendocrine system using adult zebrafish exposed to environmentally relevant concentrations of TCS (0.361−48.2 μg/L) for 21 days. Assessment of reproductive and neurotoxicity included histopathological examination and behavioral tests. Transcriptomics, proteomics analyses, and biochemical detection were employed to elucidate mechanisms underlying TCS-induced neuroendocrine disruption. Significant correlations were found between TCS exposure and estradiol, testosterone, and sex hormone-binding globulin levels in NHANES participants. In addition, TCS exposure inhibited ovary development and spermatogenesis in zebrafish. Transcriptomics and proteomics analysis revealed gender-specific key signaling and metabolism-related pathways implicated in TCS-induced reproductive toxicity. Moreover, TCS exposure induced nervous system impairment, as evidenced by histological changes and altered motor behavior, possibly associated with oxidative damage. Correlation analysis further highlighted the potential connection between endocrine system disruption and nervous system impairment following TCS exposure. Overall, this study provided evidence supporting TCS-induced endocrine disruption and offered insights into its underlying mechanisms.

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“…Swimming behavior. Multiple swimming behavior parameters were quantified by use of EthoVision XT V17 (Noldus, Wageningen, The Netherlands), a well-established zebrafish video tracking and analysis system [28]. In brief, twenty-four larvae at 120 hpf for each treatment (six for each replicate) were transferred to a 96-well plate and incubated at 28 ± 1 • C. After an initial acclimation of about one hour, the digital videos were recorded for 5 min in the phases of light and dark, respectively.…”

Section: Methodsmentioning

confidence: 99%

Developmental Toxicities in Zebrafish Embryos Exposed to Tri-o-cresyl Phosphate

Wang

Gui

et al. 2023

Water

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As a widely used plasticizer and fire retardant, tri-o-cresyl phosphate has been commonly found in global water sources, sediments and biota. However, its potential toxicity to aquatic organisms is not fully understood. Here, we assessed its developmental effects by use of a zebrafish (Danio rerio) model at tri-o-cresyl phosphate concentrations between 0.15 and 88.5 μg/L. Diverse impairments of zebrafish embryos, such as altered morphological and physical characteristics and locomotor behaviors, were observed at different tri-o-cresyl phosphate concentrations. Furthermore, swimming behaviors were significantly inhibited at tri-o-cresyl phosphate concentrations ranging from 3.0 μg/L to 88.5 μg/L. The swimming activity during light-to-dark transition significantly increased at tri-o-cresyl phosphate concentrations of 14.5 μg/L to 88.5 μg/L. Taken together, our present data help to clarify the potential developmental toxicity of tri-o-cresyl phosphate that was not yet fully recognized, and thus contribute to its environmental risk assessment.

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Analysis of the potential behavioral impact of methanol when used as a solvent: Dataset from zebrafish (Danio rerio) behavioral research

Cited by 7 publications

References 12 publications

The psychoactive effects of Bryophyllum pinnatum (Lam.) Oken leaves in young zebrafish

The psychoactive effects of Bryophyllum pinnatum (Lam.) Oken leaves in young zebrafish

Insights into Triclosan-Induced Endocrine Disruption: Evidence from the National Health and Nutrition Examination Survey and Zebrafish Models

Developmental Toxicities in Zebrafish Embryos Exposed to Tri-o-cresyl Phosphate

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