Analysis of the Relationship between MHC-DRB1 Gene Polymorphism and Hydatidosis in Kazakh Sheep

Abstract

The objective of this work was to analyze the relationship between ovine major histocompatibility complex (MHC) DRB1 gene polymorphism and genetic resistance to hydatidosis in Kazakh sheep. The Ovar (ovine MHC) class II DRB1 second exon was amplified by polymerase chain reaction (PCR) from DNA samples of 702 Kazakh sheep, including 302 sheep with hydatidosis and 400 health controls. PCR products were characterized by the restriction fragment length polymorphism (RFLP) technique using five restriction enzymes, i.e., MvaI, HaeIII, SacI, SacII and Hin1I, yielding 14 alleles and 28 genotypes. Comparing the frequency of genotypes in hydatidosis sheep with the control group, it was found that the genotype frequencies of MvaIbc, Hin1Iab, SacIIab, HaeIIIde, HaeIIIdf and HaeIIIdd in control sheep were significantly (p<0.01) higher than in hydatidosis sheep, indicating that a significant correlation existed between these genotypes and resistance to hydatidosis. Genotype frequencies of MvaIbb, SacIIaa, Hin1Ibb and HaeIIIef in sheep with hydatidosis were extremely significantly (p<0.01) higher than in the control group, and the genotype frequency of HaeIIIab was significantly higher (p<0.05), indicating that a marked correlation existed between these genotypes and susceptibility to hydatidosis. By way of analyzing haplotype with these resistant genotypes, the hydatidosis resistant haplotype MvaIbc-SacIIab-Hin1Iab of Kazakh sheep was screened out, and then verified through artificial hydatid infection in sheep. The results indicated that the infection rate of sheep with the resistant haplotype of hydatidosis was significantly lower (p<0.01) than without this resistant haplotype. It showed that the genic haplotype MvaIbc-SacIIab-Hin1Iab of Ovar-DRB1 exon 2 was the resistant haplotype of hydatidosis in Kazakh sheep.

Association of molecular polymorphism in exon 2 of Ovar-DRB1 gene with weight traits in Iranian Makuie sheep breed

“…That study compared the frequency of genotypes in hydatidosis-infected sheep with those of the control group. Their results showed that there was a positive and significant correlation between identified genotypes and resistance to hydatidosis (28). In the present study, variation in the exon 2 sequence of the DRB1 locus was studied in Iranian Makuie sheep by PCR-RFLP analysis.…”

“…Allele DRB1* 0403 and DRB1* 07012, identified in the Columbia, Polypay, and Rambouillet breeds, were positively correlated with lower OPP provirus levels. In Kazakh sheep, by using the RFLP technique, 14 alleles and 28 genotypes were detected (28). That study compared the frequency of genotypes in hydatidosis-infected sheep with those of the control group.…”

MHC-DRB1/DQB1 Gen Polimorfizmi ve Çin Merinos Koyununda Kistik Echinococcosise Dirençle İlişkisi

“…For example, Al-Ghoury determined that HLA-DR1, 8 and DR-52 are associated with resistance and that HLA-DR 16 is associated with susceptibility to EG infection in Yemeni patients [34] . Li reported that susceptibility to alveolar Echinococcosis (A.E) was significantly associated with HLA-DR4 and that the DR7 allele might confer protection against A.E in humans [35,36] .…”

Differential expressions of MHC-DQB1 mRNA in Chinese merino sheep infected with Echinococosus granuclosus

“…Since immunological events involved in the clinical course of a variety of diseases, information on MHC mRNA expression has involved in human disease, such as rheumatoid arthritis (Kerlan-Candon et al 2001), ankylosing spondylitis ), allergic vasculitis (Wang et al 2007), as well as ovine disease like lymphoid interstitial pneumonia (Woodall et al 1997). However, no systematic study has been reported on MHC genes expression in different stages of CE infection in sheep, although we previously reported that MHC genes associated with CE resistance or susceptibility in Kazakh sheep (Li et al 2010). This present study, therefore, was designed to investigate the dynamic changes of MHC-DQB1 mRNA expression in peripheral blood lymphocytes (PBLs), in Chinese merino sheep infected with Echinococosus granuclosus.…”

“…Since CE represents a considerable economic and public health significance, it is, therefore, of great importance to control the disease. And during the past decades, studies pertaining to the immunological control of CE have received much more attention (Wen and Craig 1994;Sturm et al 1995;Li et al 2010).…”