Analysis of traditional Chinese anticancer drugs by capillary electrophoresis

Liebich, H.M.; Lehmann, Rainer; Stefano, Costanza Di; Häring, Hans‐Ulrich; Kim, J.H; Kim, K.R

doi:10.1016/s0021-9673(97)00972-2

Cited by 27 publications

(13 citation statements)

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“…The HPLC method used was similar to the previous one used for the analysis of urinary nucleosides [41][42][43] except for different injection volume and standard solution concentrations. The equipment (Merck-Hitachi, Darmstadt, Germany) used comprised of a L-6200 pump, a L-3000 photo diode array detector, a 655A-40 column oven and an D-6000 interface.…”

Section: Rp-hplc Methodsmentioning

confidence: 99%

“…Urine samples were therefore collected at the same time from 15 of cancer patients from whom the corresponding sera had been studied. The urinary nucleosides from these patients were analyzed by using the previous HPLC method [41][42][43]. The comparison of urinary excretion levels between normal humans and cancer patients is given in Figure 6.…”

Section: Comparison Of Normal and Modified Nucleosides As Tumor Markementioning

confidence: 99%

“…Therefore to compare our data with existing data, it is necessary to analyze the nucleosides in serum from normal humans and cancer patients by applying the previously developed RP-HPLC method [41][42][43]. This would allow us to compare the difference between serum and urine as a sample for the tumor marker investigation.…”

Section: Introductionmentioning

confidence: 99%

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Study of normal and modified nucleosides in serum by RP-HPLC

Enderle

Liebich

et al. 2000

Chromatographia

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KeyWordsColumn liquid chromatography Nucleosides in serum Tumor markers Factor analysis SummaryModified nucleosides derived predominantly from transfer ribonucleic acid (tRNA) have been studied as possible tumor markers. In this paper a reversed-phase high-performance liquid chromatographic (RP-HPLC) method has been applied to study 15 normal and modified nucleosides in serum. The nucleoside levels in normal human serum were established, and the concentrations of 15 nucleosides in serum from 19 cancer patients were determined. It was found that the concentrations of modified nucleosides were significantly higher in patient sera. Based on 15 nucleoside concentrations in serum, factor analysis could classify correctly 90~ of cancer patients from the normal humans. Furtherwork showed that urine was slightly better than serum when determining nucleosides as biological marker candidates. More work is ongoing to determine the clinical usefulness of modified nucleosides as tumor markers.

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Section: Rp-hplc Methodsmentioning

confidence: 99%

Section: Comparison Of Normal and Modified Nucleosides As Tumor Markementioning

confidence: 99%

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Study of normal and modified nucleosides in serum by RP-HPLC

Enderle

Liebich

et al. 2000

Chromatographia

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“…Pressure, or hydrodynamic, injection is performed by applying an external force [38,42,45,48,58,71,72,96,97,111,135,136] or alternatively by raising the sample vial above the outlet causing siphoning [41,47,101,137]. Especially the former method is often used, as it is not influenced by the conductivity of the sample buffer or the mobility of the analytes to introduce precisely reproducible volumes of sample into the capillary.…”

Section: Injection Modementioning

confidence: 99%

Optimization of CZE for analysis of phytochemical bioactive compounds

Wang

2006

Electrophoresis

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Advantages of CZE such as high efficiency, low cost, short analysis time, and easy implementation result in its wide applications for analysis of phytochemical bioactive compounds (e.g. flavonoids, alkaloids, terpenoids, phenolic acid, saponins, anthraquinones and coumarins). However, several aspects, including sample preparation, separation, and detection have significant effects on CZE analysis. Therefore, optimization of these procedures is necessary for development of the method. In this review, sample preparation such as extraction method and preconcentration, separation factors including buffer type, concentration and pH, additives, voltage and temperature, as well as detection, e.g. direct and indirect UV detection, LIF and MS were discussed for optimization of CZE analysis on phytochemical bioactive compounds. The optimized strategies were also reviewed.

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“…For example, the volatile buffer salt ammonium acetate was successfully applied for this purpose by Chen et al [17], who identified berberine and two related isoquinoline alkaloids in Chinese medicine preparations using CE-electrospray-IT-MS. Also, nonaqueous buffer systems were applied for the CZE analysis of berberine in Rhizoma coptidis [18] and various Thalictrum species [19]. Whereas Liebich et al [20] used an acidic running buffer (100 mM sodium citrate, pH 2.7) for the quantitative determination of berberine in traditional Chinese anticancer drugs, basic electrolytes were used by Li et al [21] and Huitao et al [22] for the analysis of berberine in ten different Mahonia species and traditional Chinese medicinal preparations, respectively. Although the use of neutral to basic electrolytes by Li et al [21] and Huitao et al [22] was suitable for the precise quantification of isoquinoline alkaloids in crude plant matrices, the application of acidic running buffers for the CZE analysis of basic alkaloids in complex plant matrices is advantageous because the protonated alkaloids are well separated from acidic and neutral compounds.…”

Section: Introductionmentioning

confidence: 99%

Capillary zone electrophoresis as a tool for the quality control of goldenseal extracts

2005

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The root extracts of goldenseal (Hydrastis canadensis L.) are popular phytomedicines for the treatment of gastrointestinal disorders and upper respiratory tract infections. Here we describe a simple and fast capillary zone electrophoresis (CZE) method with ultraviolet detection at 225 nm for the quantification of the major goldenseal constituents, berberine and hydrastine, in herbal remedies containing goldenseal root extracts. Tritoqualine, an antihistaminic drug with a hydrastine-like phthalidisoquinoline structure, was applied as an internal standard. The running buffer was a 1:5 mixture of 500 mM ammonium acetate (adjusted to pH 3.4 with acetic acid) and methanol. Our newly developed CZE method was validated regarding limit of detection (LOD), limit of quantification, linearity, accuracy and precision. For both berberine and hydrastine, the LOD was 1.0 microg/mL and the linearity was obtained between 2.5 and 500 microg/mL. Using our newly developed method, both the alkaloids could be analysed in herbal remedies containing goldenseal root extracts within 8 min.

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Analysis of traditional Chinese anticancer drugs by capillary electrophoresis

Cited by 27 publications

References 1 publication

Study of normal and modified nucleosides in serum by RP-HPLC

Study of normal and modified nucleosides in serum by RP-HPLC

Optimization of CZE for analysis of phytochemical bioactive compounds

Capillary zone electrophoresis as a tool for the quality control of goldenseal extracts

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