Analysis of Two Clonal Lines (Embryogenic and Non-Embryogenic) of <i>Agave fourcroydes</i> Using AFLP and MSAP

Abstract

Somatic embryogenesis is a very efficient way to propagate economically important plants; however, not all genotypes within a species can be propagated using this method, as a combined effect of both genetic and epigenetic mechanisms may be involved in the response. The aim of the present study was to perform a comparative analysis of the genetic differences through amplified fragment length polymorphism (AFLP) and the epigenetic differences through methylation-sensitive amplified polymorphism (MSAP) of two Agave fourcroydes clonal lines, one highly embryogenic (K33) and the other non-embryogenic (K7). Genetic and epigenetic variabilities existed within each clonal line; however, the polymorphic profiles from the two marker systems allowed us to clearly distinguish the two clonal lines before somatic embryogenesis induction. During the induction, the changes detected were mainly 1) unmethylated fragments in the initial explants that were methylated during induction (methylation events) and 2) fragments with different methylation states in the initial explant that were unmethylated in some stages of the process (demethylation events). K33 showed greater dynamism in relation to methylation/demethylation events, while K7 presented the methylation events in a more constant range and at higher levels during all process.

Respuesta Morfogenética De Dos Especies De Agave Regeneradas in Vitro

“…Las plantas de Agave tienen dos tipos de propagación: sexual y asexual (Alfonso-León, 2014). La maduración de la planta para que ocurra la reproducción sexual, dependiendo de la especie, puede variar entre 10-25 años (Monja-Mio et al, 2018;Narváez-Suárez et al, 2016). Existen dos vías de reproducción asexual: por bulbilos, los cuales se desarrollan a partir de la zona meristemática apical que crece en la inflorescencia (Binh et al,1990) cuyo origen es de herencia materna y la propagación por hijuelos, siendo ésta última la más empleada para establecer plantaciones comerciales de agave, ya que sólo tarda 5 años para que la planta comience a producir hijuelos, contrario a la formación de bulbilos donde se debe esperar a que la planta llegue a su madurez completa (Puente-Garza et al, 2015).…”