Analysis of unknown compounds in azithromycin bulk samples with liquid chromatography coupled to ion trap mass spectrometry

Abstract

A selective reversed-phase liquid chromatography/mass spectrometry (LC/MS n ) method is described for the identification of azithromycin impurities and related substances in commercial azithromycin samples. Mass spectral data are acquired on an LCQ ion trap mass spectrometer equipped with an atmospheric pressure chemical ionization interface operated in positive ion mode. The LCQ provides on-line LC/MS n capability, making it ideally suited for identification purposes. In comparison with UV detection, this hyphenated technique provides as its main advantage efficient identification of novel substances without time-consuming isolation and purification procedures. Using this technique, six novel related substances detected in commercial azithromycin samples have been studied.

A Robust Liquid Chromatographic Method for Confirmation of Drug Stability of Azithromycin in Bulk Samples, Tablets and Suspensions

“…There are several published methods for the analysis of azithromycin such as biological [5], electroanalytical [6], spectrophotometric [7], capillary electrophoresis [8], thin layer chromatography [9] and liquid chromatography [10][11][12][13] . Liquid chromatography is the method of choice for the analysis of azithromycin in bulk samples and formulations since it is able to determine azithromycin in the presence of related substances.…”

A Robust Liquid Chromatographic Method for the Analysis of Azithromycin in Bulk Samples, Tablets and Suspensions

“…There are several published methods for the analysis of azithromycin such as biological [5], electroanalytical [6], spectrophotometric [7], capillary electrophoresis [8], thin layer chromatography [9] and liquid chromatography [10][11][12][13] . Liquid chromatography is the method of choice for the analysis of azithromycin in bulk samples and formulations since it is able to determine azithromycin in the presence of related substances.…”

Adsorptive Stripping Voltammetric Determination of Azithromycin at a Glassy Carbon Electrode Modified by Electrochemical Oxidation

“…3 The most commonly employed techniques for the determination of azithromycin in biological matrixes have been based on HPLC using electrochemical detection. 4,5 Assays reported in the literature for the measurements of the drug in pharmaceutical dosage forms include HPLC, 6,7 LC-MS, 8 and microbiological methods. 9 However, the chromatographic techniques require long experimental procedures for sample clean-up and demand expensive equipment that might not be available in many laboratories.…”