Analysis of urinary 5-hydroxyindoleacetic acid by thin layer chromatography: Elimination of interference by glyceryl guaiacolate

McGregor, Robert F.; Phillips, G. F.; Romsdahl, Marvin M.

doi:10.1016/0009-8981(72)90251-3

Cited by 3 publications

(1 citation statement)

References 6 publications

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“…Since 1970, it has been known to cause errors in laboratory determinations of 5HIAA using 1-nitroso-2-naphthol ( 54 ). However, this interference should have been eliminated in 1972 by using a modified colorimetric technique ( 55 ). Methocarbamol and mephenesin carbamate elicited false-positive tests for 5HIAA ( 56 ).…”

Section: Urinary Metabolite Of Serotonin 5 Hydroxy-indole Acetic Acimentioning

confidence: 99%

Urinary sampling for 5HIAA and metanephrines determination: revisiting the recommendations

Corcuff

Chardon

Ridah³

et al. 2017

Endocrine Connections

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ContextBiogenic amines such as 5-hydroxy-indole acetic acid (5HIAA) the main metabolite of serotonin or metanephrines (catecholamines metabolites) are used as biomarkers of neuroendocrine tumours.ObjectiveTo re-evaluate the recommendations for urinary sampling (preservatives, diet, drugs, etc.) as many of the reported analytical interferences supporting these recommendations are related to obsolete assays.MethodsBibliographic analysis of old and modern assays concerning preservation, extraction, assay and interferences.Results5HIAA may degrade as soon as urine is excreted. Thus, acids as preservatives (hydrochloric or acetic acid) have to be immediately added. Care should be taken not to decrease the pH under 2. Urine preservative for metanephrine assays is not mandatory. Diets including serotonin-, tryptophan- and dopamine-rich foods have to be avoided depending on the biomarkers investigated (bananas, plantain, nuts, etc.). Tryptophan-rich over-the-counter formulas have to be prohibited when 5HIAA has to be assayed. Acetaminophen may interfere with electrochemical detection depending on high-pressure liquid chromatography (HPLC) parameters. No interference is known with mass spectrometric assays but with the one described for metanephrines determination. Some drugs interfere however with serotonin and catecholamines secretion and/or metabolism (monoamine oxidase inhibitors, serotonin or dopamine recapture inhibitors, etc.).ConclusionRevisited recommendations are provided for the diet, the drugs and the preservatives before HPLC coupled with electrochemical and mass spectrometry assays.

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Section: Urinary Metabolite Of Serotonin 5 Hydroxy-indole Acetic Acimentioning

confidence: 99%