This study developed an eco-block for grass growth using the expanded vermiculites that absorb bacteria selected considering for the high pH and dry environments and plant growth. For the developed eco-block, a fundamental properties including compressive strength gain and water absorption and ecology characteristics were tested. The selected bacteria was Bacillus alcalophilus and Rhodoblastus acidophilus and had high concentration of 10 9 cell/mL. The expanded vermiculite that was used for shelter of bacteria was added by 7.5% and 10% replacement of the natural aggregates by volume. The developed eco-block achieved the minimum requirements specified in SPS provision and significantly effective in reducing chemical Chemical Oxygen Demand(COD) concentration and enhancing the growth of fish and plant.