Analysis on the Chlorophyll Content of Commercial Green Leafy Vegetables

Abstract

The objectives of the present study were to evaluate the chlorophyll content of green leafy vegetables found commercially and carry out a comparative investigation between in vivo and in vitro data. The chlorophyll of green leafy vegetable can be used as visible parameters of the quality of vegetables during storage, since it will be degraded gradually along with post-harvest senescence. Therefore, the development of reliable in vivo chlorophyll measurement should be advantageous rather than visual observation for the purpose of quality control and product sortation. Here, the existence of chlorophylls in ten green leafy vegetables were reported as SPAD values of a handheld SPAD-502 chlorophyll meter and % N of an Agriexpert CCN-6000 nitrogen meter (in vivo data), as well as total peak area data of HPLC measurement for chlorophyll a and b after exhaustive extraction using methanol (in vitro data). Both in vivo and in vitro measurement gave comparable grouping of vegetables with high and low content of chlorophyll. Moreover, correlation plots between SPAD values and total peak area of HPLC showed adequate linear correlation (R 2 > 0.7), revealing the potency of in vivo observation for the prediction of actual chlorophyll content in commercial leafy vegetables. SPAD values and % N presented strong linear relationship (R 2 > 0.9), in which SPAD-meter performed better detection at very low values. The calibration curve for each species of vegetable should be substantial to overcome the limiting factors of in vivo observation, such as leaf size, tissue thickness, and variation of chloroplast distribution.

Cultivar Variety and Added Potassium Influence the Nutraceutical and Antioxidant Content in Hydroponically Grown Basil (Ocimum basilicum L.)

“…We suggest that the different assayed doses of K + did not affect the photosynthetic process of the plant, and therefore all of them were sufficient for normal development, allowing not only the maintenance of the osmotic potential in cells, but also the stomata opening and closing. K + is transported through the membranes of guard cells, opening it when increasing its concentration, which is influenced the enzymatic activation and therefore a greater concentration of this nutraceutical compound [35].…”

Leaf Area Index, Chlorophyll, Photosynthesis rate of Lettuce (Lactuca sativa L) under N-organic Fertilizer

“…Some bioactive compounds in vegetables are vitamins, minerals, antioxidants, and pigments (chlorophylls and carotenoids) (Limantara et al, 2015). Lettuce (Lactuca sativa L.) is one of the most widely vegetables consumed in the world since it is a good source of fiber, but has a low in calories, fat, and sodium.…”

Phytoremediation of urea fertilizer factory wastewater by teak (Tectona grandis)

“…As an index of the chlorophyll content, the greenness of new fully expanded leaves was estimated using a SPAD-502 chlorophyll meter. The SPAD values are highly correlated to chlorophyll content (R 2 ≈ 0.9) and follow exponential or second-order polynomial functions [15].…”