The main purpose is to study analytically about the Diffusion-thermo impact on mixed convective flow of Casson fluid in a vertical channel in occurrence of porous media, uniform magnetic field and amplification. Similarity transformation is implemented to transform nonlinear coupled PDEs into ODEs. Further, obtained equations were solved using perturbation technique and studied the characters of heat, velocity and concentration of the corporeal system. The influence of nondimensional factors such as Darcy number Da, buoyancy parameter of concentration N, M2 Hartmann number, dufour number df, rate of chemical reaction γ, Schmidt number Sc, thermal buoyancy parameter λ, Prandtl number Pr, Casson parameter β, and Reynolds number R on concentration, temperature and velocity deliberated explicitly. Few important computational work reveals that the Dufour effect Df enhances the concentration, temperature and fluid flow whereas Casson fluid parameter β diminishes the profiles. The earlier work and present work have been compared for a particular case in the nonexistence of Dufour effect and porous media and were found to be coinciding.