Analytical Bioelectrochemistry

Thévenot, Daniel

doi:10.1007/978-1-4684-5098-9_19

Cited by 1 publication

(1 citation statement)

References 34 publications

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“…Second, the steady-state response time was unchanged. This result is very different from that of some previous experiments using an extracorporal blood shunt where slopes of in vitro post-calibration curves reached only 20-50% of pre-calibration ones (Thevenot, 1985;Tallagrand et al, 1988). Third, in vivo and in vitro sensitivities were usually significantly different, their ratio varying in our experiments between O-68 and 2.08.…”

Section: In Vivo Sensor Evaluationcontrasting

confidence: 90%

Study and development of multilayer needle-type enzyme-based glucose microsensors

et al. 1989

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Glucose oxidase (GOD) was covalently coupled to a cellulose acetate (CA) layer, using bovine serum albumin (BSA) and parabenzoquinone (PBQ) linkages. Prior to GOD coupling this CA layer was deposited on the platinum tip of a needle-type sensor and covered with an outer layer of polyurethane (PU). Such microsensors were found to be active, their GOD load reaching 1.6 to 3.0 micrograms mm-2 and their glucose response reaching 1 to 3 microA M-1 mm-2, even when the upper limit of their linear range reached 10-30 mM. Due to the multilayer structure and composition of these microsensors, small anions such as ascorbate were partially discriminated from neutral molecules such as hydrogen peroxide. When implanted subcutaneously in anaesthetized rats, sensor responses correlated correctly with blood glucose concentration but presented sensitivity coefficients significantly different to those determined in vitro: a 2 point calibration procedure was found necessary for in vivo experiments.

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Section: In Vivo Sensor Evaluationcontrasting

confidence: 90%