Analytical Challenges in The Detection of Peptide Hormones For Anti-Doping Purposes

Barroso, Osquel; Handelsman, David J.; Strasburger, Christian J.; Thevis, Mario

doi:10.4155/bio.12.128

Cited by 31 publications

(21 citation statements)

References 105 publications

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“…Quantification of signature peptides of the intact protein obtained via protease digestion is a commonly used approach to circumvent this problem; however, the sample preparation is time‐consuming and laborious . While the debate about the reliability of immunoassays for measuring the true concentration of IGF‐I in blood continues, MS looks to be a promising reference technique …”

mentioning

confidence: 99%

Quantification of intact human insulin‐like growth factor‐I in serum by nano‐ultrahigh‐performance liquid chromatography/tandem mass spectrometry

Lopes

Cowan

Thevis

et al. 2014

Rapid Comm Mass Spectrometry

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A quantitative method was developed for the quantification of intact IGF-I in human serum samples. The data generated provided important information for the development of a new reference method for the growth hormone biomarker test and helped create a reliable system for monitoring peptide hormones in individual athletes, a possible extension to the athlete biological passport system. Nano-electrospray has here been shown to be sufficiently robust for routine use in an analytical laboratory, allowing for the analysis of minute sample volumes.

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mentioning

confidence: 99%

Quantification of intact human insulin‐like growth factor‐I in serum by nano‐ultrahigh‐performance liquid chromatography/tandem mass spectrometry

Lopes

Cowan

Thevis

et al. 2014

Rapid Comm Mass Spectrometry

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“…119 Progressos nas técnicas de detecção e métodos de análise têm sido apresentados com o objetivo de se buscar biomarcadores de doping. 120,121 Adicionalmente, a metabolômica também é aplicada no controle de abuso de medicamentos em animais, especialmente em cavalos de corrida no hipismo. 124 além do entendimento das alterações metabó-licas resultantes da realização de atividades físicas e seus impactos na saúde, especialmente na prevenção de doenças.…”

Section: Ambientalunclassified

Metabolômica: Definições, Estado-Da-Arte E Aplicações Representativas

Canuto¹,

Costa²,

Cruz³

et al. 2017

Quim. Nova

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METABOLOMICS: DEFINITIONS, STATE-OF-THE-ART AND REPRESENTATIVE APPLICATIONS.Metabolomics is an emerging and promising omics approach used to understand biological mechanisms. By untargeted and targeted metabolomics analyses, metabolites are determined in biological samples (fluids, cells, tissues, etc.) by comparison of control groups with altered groups, undergoing different therapies, submitted to differing stress levels, dietary modulation, or promoted by a disease, or specific condition, etc., using sophisticated analytical techniques, and advanced data treatment and statistical analyses. In this review, the concepts involved in metabolomics studies were presented, describing in details all steps involved in the metabolomics workflow, for untargeted and targeted strategies. Finally, the potential of metabolomics is illustrated by applications in representative areas: clinical, environmental, food and nutrition, forensic toxicology, microbiology, parasitology, plants, and sports. Relevant reviews were compiled to characterize each of these areas, and a corresponding application of untargeted and targeted metabolomics were described.Keywords: metabolomics; untargeted metabolomics; targeted metabolomics; metabolomics workflow. INTRODUÇÃOAs ciências ômicas buscam o entendimento do funcionamento celular dos organismos e suas alterações biológicas. Fazem parte deste conjunto de ciências, a genômica (estudo da alteração dos genes), a transcriptômica (estudo das alterações dos transcritos), a proteômica (estudo das alterações das proteínas), e a metabolômica (estudo das alterações dos metabólitos). Metabólitos são produtos intermediários ou finais do metabolismo em uma amostra biológica.1 O conjunto de todos os metabólitos de baixa massa molecular (até 1500 Da), presentes ou alterados em um sistema biológico, é chamado de metaboloma (do inglês, metabolome).2 A pesquisa relacionada a metabólitos vem sendo desenvolvida há décadas, mas em 1999, Nicholson et al. definiram a metabonômica (do inglês, metabonomics), como sendo a medida quantitativa da resposta metabólica de um sistema biológico após estímulos fisiopatológicos ou modificações genéticas.3 Já o termo metabolômica (do inglês, metabolomics) foi introduzido em 2001, por Oliver Fiehn, como sendo a análise abrangente e quantitativa do metaboloma de um sistema biológico. 4 Além disso, a literatura nos apresenta outras denominações envolvendo esse campo da ciência, tais como: perfil metabólico (do inglês, metabolic profiling), descrito como sendo a análise de metabólitos previamente selecionados de rotas bioquímicas específicas, 5 a impressão digital metabólica (do inglês, metabolic fingerprinting), que é definida como sendo "uma classificação de amostras de acordo com sua origem ou sua relevância biológica", 3 e, por último, análise footprinting (ainda sem tradução para o português), para se referir aos metabólitos excretados por uma célula em condições controladas. Observa-se, portanto, uma divergência em relação às terminologias aplicadas às análises metabolômic...

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“…A range of other techniques have become available and occasionally find use in anti‐doping laboratories alongside existing methods. Biological assays can be used to detect doping directly from samples. For example, if a sample gave a positive result from an androgen receptor assay or immunoassay, it may indicate that anabolic agent(s) are present in the sample which would warrant further testing.…”

Section: Anti‐doping Screening For Designer Anabolic Steroidsmentioning

confidence: 99%

A review of designer anabolic steroids in equine sports

Waller

McLeod

2016

Drug Testing and Analysis

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In recent years, the potential for anabolic steroid abuse in equine sports has increased due to the growing availability of designer steroids. These compounds are readily accessible online in 'dietary' or 'nutritional' supplements and contain steroidal compounds which have never been tested or approved as veterinary agents. They typically have unusual structures or substitution and as a result may pass undetected through current anti-doping screening protocols, making them a significant concern for the integrity of the industry. Despite considerable focus in human sports, until recently there has been limited investigation into these compounds in equine systems. To effectively respond to the threat of designer steroids, a detailed understanding of their metabolism is needed to identify markers and metabolites arising from their misuse. A summary of the literature detailing the metabolism of these compounds in equine systems is presented with an aim to identify metabolites suitable for incorporation into screening protocols by anti-doping laboratories. The future of equine anti-doping research is likely to be guided by the incorporation of alternate testing matrices into routine screening, the improvement of in vitro technologies that can mimic in vivo equine metabolism, and the improvement of instrumentation or analytical methods that allow for the development of untargeted screening, and metabolomics approaches for use in anti-doping screening protocols.

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Analytical Challenges in The Detection of Peptide Hormones For Anti-Doping Purposes

Cited by 31 publications

References 105 publications

Quantification of intact human insulin‐like growth factor‐I in serum by nano‐ultrahigh‐performance liquid chromatography/tandem mass spectrometry

Quantification of intact human insulin‐like growth factor‐I in serum by nano‐ultrahigh‐performance liquid chromatography/tandem mass spectrometry

Metabolômica: Definições, Estado-Da-Arte E Aplicações Representativas

A review of designer anabolic steroids in equine sports

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