Analytical characterization of an assay designed to detect and identify diverse agents of disseminated viral infection

Metzgar, David; Lovari, Robert; Ray, Keith P.; Baynes, Darcie; Drapp, Darren; Frinder, Mark; Vijesurier, Roy; Stemler, Michelle; Ofsaiof, Reuben; Carolan, Heather E.; Welk, Joseph F.; Toleno, Donna M.; Ranken, Raymond; Hall, Thomas A.; Massire, Christian; Sampath, Rangarajan; Blyn, Lawrence B.; Goveia, Jon; Schneider, George J.

doi:10.1016/j.jcv.2013.12.005

Cited by 3 publications

(6 citation statements)

References 36 publications

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“…For a variety of viral pathogens, PCR/ESI-MS sensitivity is 94% and specificity is 98% ( 9 ). In this case, PCR/ESI-MS detected a human enterovirus from the right middle lobe and left lingular segment BAL fluid samples.…”

mentioning

confidence: 99%

Enterovirus D68–Associated Acute Respiratory Distress Syndrome in Adult, United States, 2014

Farrell¹,

Ikladios²,

Wylie³

et al. 2015

Emerg. Infect. Dis.

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mentioning

confidence: 99%

Enterovirus D68–Associated Acute Respiratory Distress Syndrome in Adult, United States, 2014

Farrell¹,

Ikladios²,

Wylie³

et al. 2015

Emerg. Infect. Dis.

Self Cite

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“…Genus Enterovirus (34, 12.7%) was the second-most frequently detected virus, including rhinovirus (25, 9.4%), enterovirus (8, 3.0%), and 1 culturenegative case matching for both rhinovirus and enterovirus. hCoV (13,4.9%), hMPV (10, 3.7%), adenovirus (6, 2.2%), RSV (6, 2.2%), and parainfluenza (4, 1.5%) were detected in small proportions of cases. Simultaneous detection of more than 1 respiratory virus was observed in 11 (4.1%) patients, and rhinovirus (5 cases) was most likely to be codetected with another respiratory virus ( Table 2).…”

Section: Virus Detection By Pcr-esi/msmentioning

confidence: 97%

“…The PLEX-ID Respiratory Virus assay detects human adenovirus, hCoV, hMPV, influenza A and B, parainfluenza types 1 to 3, and RSV, 6 whereas the PLEX-ID Broad Viral I assay detects human adenovirus, enterovirus, rhinovirus, BK and JC polyomavirus, parvovirus B19, HSV-1 and -2, VZV, Epstein-Barr virus (EBV), CMV, and human herpesvirus (HHV)-8. 13,14 In this study, respiratory viruses refer to adenovirus, hCoV, hMPV, influenza, parainfluenza, RSV, enterovirus, and rhinovirus. Nucleic acid amplification and analyses of PCR products were conducted using the PCR/ESI-MS platform (PLEX-ID, Abbott Laboratories) following the manufacturer's instructions, with test turnaround time from sample to result within 6 to 8 hours.…”

Section: Virus Detection and Identification By Pcr/esi-msmentioning

confidence: 99%

“…Nucleic acid amplification and analyses of PCR products were conducted using the PCR/ESI-MS platform (PLEX-ID, Abbott Laboratories) following the manufacturer's instructions, with test turnaround time from sample to result within 6 to 8 hours. 8,13 The PCR/ESI-MS analyses included automated PCR desalting, ESI-MS signal acquisition, spectral analysis, and data reporting. Organism identification was based on the total mass and base compositions of the PCR amplicons compared with those in the molecular signature database established by the PLEX-ID manufacturer.…”

Section: Virus Detection and Identification By Pcr/esi-msmentioning

confidence: 99%

“…Organism identification was based on the total mass and base compositions of the PCR amplicons compared with those in the molecular signature database established by the PLEX-ID manufacturer. 6,8,13,14 Samples in which PCR/ESI-MS results disagreed with culture results at the species level were reexamined by a second molecular method. For enteroviruses, rhinovirus was differentiated from enterovirus using a conventional PCR sequencing analysis with the previously described primers (Rhinovirus s1 and as) and a BLAST search.…”

Section: Virus Detection and Identification By Pcr/esi-msmentioning

confidence: 99%

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Viral Respiratory Tract Infections in Adult Patients Attending Outpatient and Emergency Departments, Taiwan, 2012–2013

Shih

Wang

et al. 2015

Medicine

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Viral etiologies of respiratory tract infections (RTIs) have been less studied in adult than in pediatric populations. Furthermore, the ability of PCR/electrospray ionization mass spectrometry (PCR/ESI-MS) to detect enteroviruses and rhinoviruses in respiratory samples has not been well evaluated. We sought to use PCR/ESI-MS to comprehensively investigate the viral epidemiology of adult RTIs, including testing for rhinoviruses and enteroviruses.Nasopharyngeal or throat swabs from 267 adults with acute RTIs (212 upper RTIs and 55 lower RTIs) who visited a local clinic or the outpatient or emergency departments of a medical center in Taiwan between October 2012 and June 2013 were tested for respiratory viruses by both virus isolation and PCR/ESI-MS. Throat swabs from 15 patients with bacterial infections and 27 individuals without active infections were included as control samples.Respiratory viruses were found in 23.6%, 47.2%, and 47.9% of the 267 cases by virus isolation, PCR/ESI-MS, and both methods, respectively. When both methods were used, the influenza A virus (24.3%) and rhinoviruses (9.4%) were the most frequently identified viruses, whereas human coronaviruses, human metapneumovirus (hMPV), enteroviruses, adenoviruses, respiratory syncytial virus, and parainfluenza viruses were identified in small proportions of cases (<5% of cases for each type of virus). Coinfection was observed in 4.1% of cases. In the control group, only 1 (2.4%) sample tested positive for a respiratory virus by PCR/ESI-MS. Patients who were undergoing steroid treatment, had an active malignancy, or suffered from chronic obstructive pulmonary disease (COPD) were at risk for rhinovirus, hMPV, or parainfluenza infections, respectively. Overall, immunocompromised patients, patients with COPD, and patients receiving dialysis were at risk for noninfluenza respiratory virus infection. Rhinoviruses (12.7%), influenza A virus (10.9%), and parainfluenza viruses (7.3%) were the most common viruses involved in the 55 cases of lower RTIs. The factors of parainfluenza infection, old age, and immunosuppression were independently associated with lower RTIs.In conclusion, PCR/ESI-MS improved the diagnostic yield for viral RTIs. Non-influenza respiratory virus infections were associated with patients with comorbidities and with lower RTIs. Additional studies that delineate the clinical need for including non-influenza respiratory viruses in the diagnostic work-up in these populations are warranted.

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The clinical use of parvovirus B19 assays: recent advances

Gallinella

2018

Expert Review of Molecular Diagnostics

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Parvovirus B19 (B19V), a single-stranded DNA virus in the family Parvoviridae, is a human pathogenic virus, characterized by a selective but not exclusive tropism for erythroid progenitor cells. Widely diffuse, it is responsible for an ample range of clinical manifestations, whose characteristics and outcomes depend on the interplay between the viral properties and the physiological and immune status of the infected individuals. The complexity of virus-host relationship and the diversity of the clinical course of infection pose a diagnostic challenge that may require non-trivial solutions. Areas covered: The review includes an updated description of the course of B19V infection in its complexity and diversity of pathogenetic mechanisms, discusses the consequent requirements for different and appropriated diagnostic approaches, presents the main diagnostic techniques, more recent technical advancements, and their application to the diverse clinical situations. Expert commentary: The complex scenario of the infectious process and the diversity in possible pathogenetic mechanisms make necessary a multi-parametric approach for an accurate and informative laboratory diagnosis of B19V infection, combining as much as possible the molecular detection of viral components, mainly viral DNA, to commonly followed immunological detection of virus-specific antibodies and a critical assessment of laboratory findings.

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Analytical characterization of an assay designed to detect and identify diverse agents of disseminated viral infection

Cited by 3 publications

References 36 publications

Enterovirus D68–Associated Acute Respiratory Distress Syndrome in Adult, United States, 2014

Enterovirus D68–Associated Acute Respiratory Distress Syndrome in Adult, United States, 2014

Viral Respiratory Tract Infections in Adult Patients Attending Outpatient and Emergency Departments, Taiwan, 2012–2013

The clinical use of parvovirus B19 assays: recent advances

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