Analytical Evaluation of Visualizing Reagents Used to Detect Tocopherol and Tocopherol Acetate on Thin Layer

Detection Progress of Selected Drugs in TLC

“…The limit of detection (detectability), detection index, broadening index, modified contrast index, densitometric visualizing index, and linearity range were determined and tested for estradiol, tocopherol, tocopherol acetate, ibuprofen, stearic acid, stearyl alcohol, and salicylanilide detected by the use of visualizing reagents. The broadening index [41, 42] was defined as $\begin{array}{c}{I}_{\text{broad}}=\frac{a}{p_2}\times \mathrm{1000}\hspace{0.17em}\left[\frac{\mu \text{g}}{\text{AU}}\right],\end{array}$ where a is the mass [ μ g] of the detected substance and p 2 is the spot area [AU] of a    μ g of this analyzed substance.…”

“…Today the area of chromatographic spot is determined by densitometric analysis. Therefore, Pyka et al modified the above-mentioned indexes and also proposed new indexes which characterized the visualizing effects [ 41 – 45 ]. The limit of detection (detectability), detection index, broadening index, modified contrast index, densitometric visualizing index, and linearity range were determined and tested for estradiol, tocopherol, tocopherol acetate, ibuprofen, stearic acid, stearyl alcohol, and salicylanilide detected by the use of visualizing reagents.…”

“…New visualizing reagents, namely, gentian violet, methylene violet, methylene blue, methyl green, malachite green, and Janus blue, have been used to detect estradiol, tocopherol, tocopherol acetate, ibuprofen, stearic acid, and stearyl alcohol [ 41 – 43 , 45 ]. Brilliant green was applied to detect salicylanilide [ 44 ].…”

“…Barton's reagent, rhodamine B, and sulphuric acid were administered as the comparative visualizing reagents [ 43 ]. (±)- α -Tocopherol and (+)- α -tocopherol acetate were chromatographed on silica gel 60 using toluene as mobile phase [ 41 ]. Rhodamine B and 2,2′-bipyridine-iron (III) chloride reagent were applied as the comparative visualizing reagents.…”

“…The best detection way of (+)- α -tocopherol acetate was densitometric method without using a visualizing reagent, whereas among all studied new visualizing reagents, gentian violet, methyl green, and Janus blue were the best to detect (+)- α -tocopherol acetate. These visualizing reagents have similar detection properties of (+)- α -tocopherol acetate in relation to rhodamine B [ 41 ]. The best detection way of ibuprofen was densitometric method without using a visualizing reagent.…”