Analytical Gradient Polyacrylamide Gel-Crossed Line Immunoelectrophores. A Technique for Locating Species Specific<i>Mycobacterium Tuberculosis</i>H<sub>37</sub>Rv Antigens in Polyacrylamide Gel Columns

Johnson, Alva H.; Wright, George L.; Chaparas, Sotiros D.; Mancuso, Daniel

doi:10.3109/08820138009052998

Cited by 1 publication

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“…21 22 The acid fast organisms grown from the blood of patients with sarcoidosis also stained positively with the H37RV monoclonal antibody which was raised against M tuberculosis H37RV whole cell antigen. 25 The antibody is weakly reactive to mycobacteria other than M tuberculosis and does not react with Gram negative or Gram positive bacteria. Our findings therefore confirm that CWDF isolated from patients with sarcoidosis belong to the mycobacterial family.…”

Section: Discussionmentioning

confidence: 97%

Growth of acid fast L forms from the blood of patients with sarcoidosis.

Almenoff¹,

Johnson²,

Lesser³

et al. 1996

Thorax

101

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Background -Acid fast cell wall deficient forms (CWDF) of bacteria have been grown from blood, bronchial washings, and ocular anterior chamber fluid from patients with sarcoidosis. A monoclonal antibody raised against Mycobacterium tuberculosis whole cell antigen (H37RV) was used to characterise further CWDF grown from the blood of patients with sarcoidosis. Methods -Blood from 20 patients with active sarcoidosis and from 20 controls was cultured using methods favourable for the growth of CWDF. Isolates were further characterised by indirect fluorescent antibody analysis using a monoclonal antibody highly reactive with M tuberculosis. Results -CWDF were grown from the blood of 19 of 20 subjects with sarcoidosis. All isolates stained positively with the monoclonal antibody and with a modified Kinyoun stain. No organisms were grown from the blood of controls.Conclusions -These data demonstrate that CWDF can be grown from the blood of nearly all patients with active sarcoidosis. The results confirm that the organisms are mycobacterial in origin and are similar, if not identical, to M tuberculosis. Their role in the pathogenesis of sarcoidosis is unknown. (Thorax 1996;51:530-533)

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Section: Discussionmentioning

confidence: 97%