Analytical methods for detection and determination of genetically modified organisms in agricultural crops and plant-derived food products

Anklam, Elke; Gadani, Ferruccio; Heinze, Petra; Pijnenburg, Hans; Eede, Guy Van den

doi:10.1007/s002170100415

Cited by 249 publications

(155 citation statements)

References 6 publications

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“…PCR is used in the growing analytical approach for GM organism detection (Holst-Jensen and Berdal, 2004). Among DNA-based methods, PCR technology is preferred by many analytical laboratories interested in the detection of GM organisms because of its high sensitivity, specificity, and wide range of gene constructs (Ahmed, 2002;Anklam et al, 2002;Holst-Jensen et al, 2003). PCR-based detection can be evaluated in at least four categories related to the level of specificity namely screen-specific, gene-specific, construct-specific, and event-specific evaluation.…”

Section: Introductionmentioning

confidence: 99%

Detection of genetically modified maize and soybean in feed samples

Meriç¹,

Çakır²,

Turgut-Kara³

et al. 2014

Genet. Mol. Res.

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ABSTRACT. Despite the controversy about genetically modified (GM) plants, they are still incrementally cultivated. In recent years, many food and feed products produced by genetic engineering technology have appeared on store shelves. Controlling the production and legal presentation of GM crops are very important for the environment and human health, especially in terms of long-term consumption. In this study, 11 kinds of feed obtained from different regions of Turkey were used for genetic analysis based on foreign gene determination. All samples were screened by conventional polymerase chain reaction (PCR) technique for widely used genetic elements; cauliflower mosaic virus 35S promoter (CaMV35S promoter), and nopaline synthase terminator (T-NOS) sequences for GM plants. After determination of GM plantcontaining samples, nested PCR and conventional PCR analysis were performed to find out whether the samples contained Bt176 or GTS-40-3-2 for maize and soy, respectively. As a result of PCR-based GM plant analysis, all samples were found to be transgenic. Both 35S-and NOS-containing feed samples or potentially Bt176-containing samples, in other words, were analyzed with Bt176 insect resistant cryIAb gene- Genetic modified organism detection in feed samples specific primers via nested PCR. Eventually, none of them were found Bt176-positive. On the other hand, when we applied conventional PCR to the same samples with the herbicide resistance CTP4-EPSPS construct-specific primers for transgenic soy variety GTS-40-3-2, we found that all samples were positive for GTS-40-3-2.

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Section: Introductionmentioning

confidence: 99%

Detection of genetically modified maize and soybean in feed samples

Meriç¹,

Çakır²,

Turgut-Kara³

et al. 2014

Genet. Mol. Res.

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show abstract

“…The aim of a validation process is to show that the combination of steps necessary to perform a GMO detection, including DNA extraction, amplification, and the analysis of the amplification products, gives precise, reproducible and accurate results for a given 25 procedure and a given food matrix. 98 The parameters used for validation are different for qualitative or quantitative methods. Validation of an analytical qualitative method involves the study of the specificity, sensitivity, precision and robustness, while for quantitative methods limit of quantification and linearity range are also accounted for.…”

Section: Validation Of Methodsmentioning

confidence: 99%

“…Validation of an analytical qualitative method involves the study of the specificity, sensitivity, precision and robustness, while for quantitative methods limit of quantification and linearity range are also accounted for. 98 Very few of the PCR-based detection methods published so far, either qualitative of quantitative, have passed through a validation process including inter-laboratories ringtrials. The results for two collaborative studies for qualitative GMO-detection methods have been published.…”

Section: Validation Of Methodsmentioning

confidence: 99%

Detection of Genetically Modified Organisms in Foods by DNA Amplification Techniques

García‐Cañas

Cifuentes

González

2004

Critical Reviews in Food Science and Nutrition

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“…Several recent review articles contain detailed descriptions of the current methods of sampling and analysis of GMO contents (e.g., Anklam et al 2002a;Bonfini et al 2002;Holst-Jensen et al 2003). The methods are therefore only described briefly below.…”

Section: Methods Of Sampling and Analysismentioning

confidence: 99%

Control of GMO Content in Seed and Feed

2004

TemaNord

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Analytical methods for detection and determination of genetically modified organisms in agricultural crops and plant-derived food products

Cited by 249 publications

References 6 publications

Detection of genetically modified maize and soybean in feed samples

Detection of genetically modified maize and soybean in feed samples

Detection of Genetically Modified Organisms in Foods by DNA Amplification Techniques

Control of GMO Content in Seed and Feed

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