Analytical Methods for Quality and Quantity Control of Food Supplements, Containing Caffeine

Hadzhieva, Bozhidarka; Pankova, Stefka A.; Ivanov, Kalin; Ivanova, Stanislava; Doncheva, D; Pencheva, Ivanka

doi:10.11648/j.ijnfs.s.2015040101.13

IJNFS

2015

DOI: 10.11648/j.ijnfs.s.2015040101.13

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Analytical Methods for Quality and Quantity Control of Food Supplements, Containing Caffeine

Bozhidarka Hadzhieva¹,

Stefka A. Pankova²,

Kalin Ivanov³

et al.

Abstract: Many beverages such as soft drinks, coffee and tea contain the mild stimulant caffeine (C 8 H 10 N 4 O 2). The caffeine content varies widely from about 100 µg/mL (100 ppm) in sodas to over 1000 µg/mL in certain types of coffee. For this reason the caffeine and the content in they need to be analyzed. A rapid and selective high-performance liquid chromatographic (HPLC) method is developed for the separation and determination of caffeine.

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Cited by 3 publications

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Wild and Cultivated Cherries – Antioxidant Capacity Analysis

Topolov

Dermendjiev

Phortunov

2017

ESJ

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Cherries are fruit that is rich in powerful antioxidants (anthocyanin) and many different stimulating good health substances. SCOPE: Determination and analysis of pH, total phenols, antioxidant activity (radical trapping activity) total anthocyanin of wild growing and cultivated varieties of cherries. Research Methods Applied: -Systematic approach and critical analysis of the available scientific periodicals; -Spectrophotometric method for determination of adsorption and standard Gallic acid; -DPPH method for determining the antioxidant activity (radical trapping activity). The results presented are the mean values of at least three parallel tests. The total phenols of the samples we studied are in the range of 0.57 to 1.67 mg GAE / g fresh weight. Total anthocyanin in the samples we tested was in the range of 0.02 to 0.44 mg / g of fresh weight. Antioxidant activity of 7.88 to 16.20 mol TE / g fresh weight pH of 3.70 to 4.19. CONCLUSION: Common anthocyanin is highest in early sorts of cultivated cherries. In later ripening varieties of cultivated cherries, the value of common anthocyanin is significantly lower. In addition, this indicator depends on the altitude of the cultivated cherry. The antioxidant activity is highest in the variety Wild Cherry (Banya, Karlovo), Early Cherry Van (Radilovo), Van variety (Isperihovo) and Cartilage Bing variety or Bigato

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Wild and Cultivated Cherries – Antioxidant Capacity Analysis

Topolov

Dermendjiev

Phortunov

2017

ESJ

View full text Add to dashboard Cite

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Determination of the content of caffeine and taurine in energy drinks and dietetic products by hplc method

Nedeljković¹,

Pavlović²,

Dilber³

et al. 2017

Hrana i ishrana

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Spectrophotometric determination of caffeine in energy drinks and teas

Marković¹,

Petrović²,

Đogo-Mračević³

et al. 2020

Medicinski podmladak

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Uvod: Kofein je prirodni alkaloid, izolovan iz više od 60 biljnih vrsta. Preko 80% svetske populacije svakodnevno unosi kofein u organizam kroz konzumaciju kafe, čajeva, čokolade i, u poslednje vreme, energetskih pića. Ne treba zanemariti da kao farmaceutski aktivna supstanca jakog delovanja ulazi u sastav mnogih lekova, koji se na tržištu Republike Srbije izdaju bez lekarskog recepta. Sem toga, kofein je psihoaktivni stimulans, blag je diuretik i pokazuje interakciju sa nekim lekovima usled indukcije CYP1 i CYP2 izoenzima zbog čega je potrebno voditi računa o količini koja se unese u organizam. U skladu sa tim, važno je i da sadržaj kofeina u energetskim pićima odgovara podacima koje proizvođači navode na deklaraciji. Cilj: Cilj ovog rada bio je spektrofotometrijsko sadržaja kofeina u uzorcima energetskih pića i čajeva, proizvođača dostupnih na tržištu R. Srbije. Materijal i metode: Kofein je određivan metodom UV-VIS spektrofotometrije u devet vrsta energetskih pića i po četiri uzorka crnog i zelenog čaja. Rezultati: U uzorcima energetskih pića određeni sadržaj kofeina je u opsegu od 26,81 do 35,44 mg/100 mL i u skladu je sa deklarisanim sadržajem. Sadržaj kofeina u uzorcima čajeva varira u odnosu na vrstu (11,27-22,61 mg/100 mL zeleni i 13,85-22,73 mg/100 mL crni), ali je vezan i za poreklo, uslove gajenja i proces prerade čajeva. Zaključak: UV/VIS spektrofotometrija se pokazala kao brza, precizna i jednostavna metoda za određivanje kofeina u energetskim pićima i čajevima. Imajući u vidu dobijene rezultate, važno je istaći da unos kofeina kroz uobičajenu dnevnu konzumaciju čajeva i energetskih pića ne bi trebalo da utiče negativno na zdravlje ljudi. Svakako treba imati u vidu da se ovaj alkaloid dodatno može uneti i putem drugih izvora, što je posebno važno za određene grupe pacijenata. Ključne reči: kofein, čaj, energetska pića, UV/VIS spektrofotometrija

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Analytical Methods for Quality and Quantity Control of Food Supplements, Containing Caffeine

Cited by 3 publications

References 20 publications

Wild and Cultivated Cherries – Antioxidant Capacity Analysis

Wild and Cultivated Cherries – Antioxidant Capacity Analysis

Determination of the content of caffeine and taurine in energy drinks and dietetic products by hplc method

Spectrophotometric determination of caffeine in energy drinks and teas

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