Analytical Methods to Characterize and Purify Polymeric Nanoparticles

Fornaguera, Cristina; Solans, Conxita

doi:10.1155/2018/6387826

Cited by 28 publications

(18 citation statements)

References 50 publications

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“…Chromatographic method development and validation play an important role in the design, development and manufacture of nanosized pharmaceuticals due to their ability to separate and quantify several analytes of interest from the components of the nanocarriers. Indeed, the quantification of the pharmaceutically active compounds is required for the study of the nanosystems encapsulation efficiency, drug release kinetics, as well as stability and even for the investigation of their interaction with biological interfaces [ 47 ].…”

Section: Discussionmentioning

confidence: 99%

Curcumin and Quercetin-Loaded Nanoemulsions: Physicochemical Compatibility Study and Validation of a Simultaneous Quantification Method

et al. 2020

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Biphasic oil/water nanoemulsions have been proposed as delivery systems for the intranasal administration of curcumin (CUR) and quercetin (QU), due to their high drug entrapment efficiency, the possibility of simultaneous drug administration and protection of the encapsulated compounds from degradation. To better understand the physicochemical and biological performance of the selected formulation simultaneously co-encapsulating CUR and QU, a stability test of the compound mixture was firstly carried out using X-ray powder diffraction and thermal analyses, such as differential scanning calorimetry (DSC) and thermogravimetric analyses (TGA). The determination and quantification of the encapsulated active compounds were then carried out being an essential parameter for the development of innovative nanomedicines. Thus, a new HPLC–UV/Vis method for the simultaneous determination of CUR and QU in the nanoemulsions was developed and validated. The X-ray diffraction analyses demonstrated that no interaction between the mixture of active ingredients, if any, is strong enough to take place in the solid state. Moreover, the thermal analysis demonstrated that the CUR and QU are stable in the nanoemulsion production temperature range. The proposed analytical method for the simultaneous quantification of the two actives was selective and linear for both compounds in the range of 0.5–12.5 µg/mL (R2 > 0.9997), precise (RSD below 3%), robust and accurate (recovery 100 ± 5 %). The method was validated in accordance with ICH Q2 R1 “Validation of Analytical Procedures” and CDER-FDA “Validation of chromatographic methods” guideline. Furthermore, the low limit of detection (LOD 0.005 µg/mL for CUR and 0.14 µg/mL for QU) and the low limit of quantification (LOQ 0.017 µg/mL for CUR and 0.48 µg/mL for QU) of the method were suitable for the application to drug release and permeation studies planned for the development of the nanoemulsions. The method was then applied for the determination of nanoemulsions CUR and QU encapsulation efficiencies (> 99%), as well as for the stability studies of the two compounds in simulated biological fluids over time. The proposed method represents, to our knowledge, the only method for the simultaneous quantification of CUR and QU in nanoemulsions.

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Section: Discussionmentioning

confidence: 99%

Curcumin and Quercetin-Loaded Nanoemulsions: Physicochemical Compatibility Study and Validation of a Simultaneous Quantification Method

et al. 2020

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“…Several methods of purication can be used, but centrifugation is practical to perform on small scale experiments. 100,101 In this work, three different centrifugation speeds were tested, 10 000 rpm (9391 Â g), 15 000 rpm (21 130 Â g), and 20 000 rpm (37 565 Â g). For this study, the particles were prepared by the technique of emulsication with the following conditions, 10 mg mL À1 of P17A in the organic phase, 5% of C PVA , 0.167 of F os , 400 of A rpm , and 75% of sonication amplitude.…”

Section: Purication Processmentioning

confidence: 99%

PLGA nanoparticle preparations by emulsification and nanoprecipitation techniques: effects of formulation parameters

et al. 2020

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“…Differential scanning calorimetry (DSC) is a technique that is used to study the melting/crystallisation behaviour, solid-solid reaction, and specific heat of a sample [ 53 ]. It can also be used to deduce the structure and stability of nanomaterials, as well as their conformation, as material transitions will vary as a subject of nanomaterial composition [ 138 ]. The technique measures the difference in heat flow between a reference and sample [ 139 ].…”

Section: Structural Morphological and Thermal Characterization Tmentioning

confidence: 99%

Instrumental Techniques for Characterization of Molybdenum Disulphide Nanostructures

Ramohlola

Iwuoha

Hato

et al. 2020

Journal of Analytical Methods in Chemistry

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The excellent chemical and physical properties of materials (nanomaterials) with dimensions of less than 100 nm (nanometers) resulted in researchers and industrialists to have great interest in their discovery and applications in various systems/applications. As their sizes are reduced to nanoscale, these nanomaterials tend to possess exceptional properties differing from those of their bulk counterparts; hence, they have found applications in electronics and medicines. In order to apply them in those applications, there is a need to synthesise these nanomaterials and study their structural, optical, and electrochemical properties. Among several nanomaterials, molybdenum disulphide (MoS2) has received a great interest in energy applications due to its exceptional properties such as stability, conductivity, and catalytic activities. Hence, the great challenge lies in finding the state-of-the-art characterization techniques to reveal the different properties of MoS2 nanostructures with great accuracy. In this regard, there is a need to study and employ several techniques to accurately study the surface chemistry and physics of the MoS2 nanostructures. Hence, this review will comprehensively discuss a detailed literature survey on analytical techniques that can be used to study the chemical, physical, and surface properties of MoS2 nanostructures, namely, ultraviolet-visible spectroscopy (UV-vis), photoluminescence spectroscopy (PL), Fourier transform infrared spectroscopy (FTIR), Raman spectroscopy, time-of-flight secondary ion mass spectroscopy (TOF-SIMS), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), scanning and transmission electron microscopies (SEM and TEM), atomic force microscopy (AFM), energy dispersive X-ray spectroscopy (EDS/X), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), and electroanalytical methods which include linear sweep (LSV) and cyclic (CV) voltammetry and electrochemical impedance spectroscopy (EIS).

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Analytical Methods to Characterize and Purify Polymeric Nanoparticles

Cited by 28 publications

References 50 publications

Curcumin and Quercetin-Loaded Nanoemulsions: Physicochemical Compatibility Study and Validation of a Simultaneous Quantification Method

Curcumin and Quercetin-Loaded Nanoemulsions: Physicochemical Compatibility Study and Validation of a Simultaneous Quantification Method

PLGA nanoparticle preparations by emulsification and nanoprecipitation techniques: effects of formulation parameters

Instrumental Techniques for Characterization of Molybdenum Disulphide Nanostructures

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