Analytical Studies on the Development of High-Performance Liquid Chromatographic Methods with Fluorescence or Chemiluminescence Detections and Their Practical Applications

Abstract

This review describes a comprehensive investigation focusing on syntheses of analytical reagents, followed by their utilization in development of analytical methods, and leading to practical applications for rational use of medicaments centering on abused drugs. Many kinds of analytical reagents for fluorescence and chemiluminescence detections have been synthesized with the intention of improving sensitivity. By properly combining the developed analytical reagents with an HPLC separation technique, one could determine ultra-small amounts of abused drugs such as stimulants, narcotics and obesity drugs in various matrices. Furthermore, chiral analyses of some abused drugs and evaluation of their potential for drug-drug interaction were also performed. The developed methods might be useful for forensic and toxicological studies on drug abuse. Also, the results obtained in our study might contribute to the prediction of and the protection of human health from risks of abused drugs.

Detection of Scopolamine Hydrobromide via Surface-enhanced Raman Spectroscopy

“…3,4 The Centers for Disease Control and Prevention (CDC) in the United States found that drug abuse increased by 23% between 2010 and 2014. In 2014, the death toll was more than 47000.…”

Evaluation of Dissolution of Rifaximin and Its Importance

“…A practical and accurate analysis method is a primary step in the rational use of pharmaceuticals. 16 Therefore, a dissolution method to tablets of rifaximin has been developed and validated in this work.…”

Development and Application of Sensitive Methods with Luminescence Detections for Determination of Biologically Active Compounds

“…Tel./Fax: +81-95-819-2450; E-mail: naka-ken@nagasaki-u.ac.jp sitive HPLC-FL and -CL methods of biologically interesting compounds including bioactive components and drugs of abuse (e.g., stimulants, narcotics and anorectics) in various matrices such as urine, plasma and hair using FL labels such as 4-(4,5-diphenyl-1H-imidazol-2-yl)benzoyl chloride (DIB-Cl) and 4-(N,N-dimethylaminosulphonyl)-7-fluoro-2,1,3-benzoxadiazole (DBD-F). 1) Representative FL labeling reagents and target compounds discussed in this review are shown in Fig. 1.…”