Analytical Techniques in Neuroscience: Recent Advances in Imaging, Separation, and Electrochemical Methods

Ganesana, Mallikarjunarao; Lee, Scott T.; Wang, Ying; Venton, B. Jill

doi:10.1021/acs.analchem.6b04278

Cited by 114 publications

(88 citation statements)

References 232 publications

(503 reference statements)

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“…Analytical techniques such as microdialysis and electrochemical micro-sensors, have provided useful insights about neuromodulator presence (12, 13), but suffer from poor spatial and/or temporal resolution, and cannot be targeted to cells of interest. Optical approaches, such as injected cell-based systems (CNiFERs) (14) and reporter gene-based iTango (15) can reveal dopamine release with high molecular specificity.…”

mentioning

confidence: 99%

Ultrafast neuronal imaging of dopamine dynamics with designed genetically encoded sensors

Patriarchi

Cho

Merten

et al. 2018

Science

897

892

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Neuromodulatory systems exert profound influences on brain function. Understanding how these systems modify the operating mode of target circuits requires measuring spatiotemporally precise neuromodulator release. We developed dLight1, an intensity-based genetically encoded dopamine indicator, to enable optical recording of dopamine dynamics with high spatiotemporal resolution in behaving mice. We demonstrated the utility of dLight1 by imaging dopamine dynamics simultaneously with pharmacological manipulation, electrophysiological or optogenetic stimulation, and calcium imaging of local neuronal activity. dLight1 enabled chronic tracking of learning-induced changes in millisecond dopamine transients in striatum. Further, we used dLight1 to image spatially distinct, functionally heterogeneous dopamine transients relevant to learning and motor control in cortex. We also validated our sensor design platform for developing norepinephrine, serotonin, melatonin, and opioid neuropeptide indicators.

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mentioning

confidence: 99%

Ultrafast neuronal imaging of dopamine dynamics with designed genetically encoded sensors

Patriarchi

Cho

Merten

et al. 2018

Science

897

892

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“…Electrochemistry is a powerful tool in understanding neurotransmission, due to the spatial and temporal superiority over other techniques [77]. Carbon fiber electrodes are widely used to monitor neurotransmitters, catecholamines, and their metabolites, with a huge body of work focused on dopamine.…”

Section: Functional and Chemical Specific Probes For Secmmentioning

confidence: 99%

Advances and Perspectives in Chemical Imaging in Cellular Environments Using Electrochemical Methods

Lazenby

White

2018

Chemosensors

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This review discusses a broad range of recent advances (2013)(2014)(2015)(2016)(2017) in chemical imaging using electrochemical methods, with a particular focus on techniques that have been applied to study cellular processes, or techniques that show promise for use in this field in the future. Non-scanning techniques such as microelectrode arrays (MEAs) offer high time-resolution (<10 ms) imaging; however, at reduced spatial resolution. In contrast, scanning electrochemical probe microscopies (SEPMs) offer higher spatial resolution (as low as a few nm per pixel) imaging, with images collected typically over many minutes. Recent significant research efforts to improve the spatial resolution of SEPMs using nanoscale probes and to improve the temporal resolution using fast scanning have resulted in movie (multiple frame) imaging with frame rates as low as a few seconds per image. Many SEPM techniques lack chemical specificity or have poor selectivity (defined by the choice of applied potential for redox-active species). This can be improved using multifunctional probes, ion-selective electrodes and tip-integrated biosensors, although additional effort may be required to preserve sensor performance after miniaturization of these probes. We discuss advances to the field of electrochemical imaging, and technological developments which are anticipated to extend the range of processes that can be studied. This includes imaging cellular processes with increased sensor selectivity and at much improved spatiotemporal resolution than has been previously customary.

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“…ECD is also much more specific for the monoamines than MS detectors. Therefore, although in recent decades several other detectors have been tested for the quantification of such derivatives, high-performance liquid chromatography (HPLC) with ECD is still considered one of the best choices, indicated by the high number of reports (Allen, Rednour, Shepard, & Pond, 2017;Bidel et al, 2016;Birbeck & Mathews, 2013;Ferry, Gifu, Sandu, Denoroy, & Parrot, 2014;Ganesana, Lee, Wang, & Venton, 2017;Nguyen, Aerts, Van Dam, & De Deyn, 2010;Parrot, Neuzeret, & Denoroy, 2011;Schou-Pedersen, Hansen, Tveden-Nyborg, & Lykkesfeldt, 2016;Smith, Schwartz, & Lucot, 2013;Tsunoda, 2006).…”

Section: Introductionmentioning

confidence: 99%

Comprehensive analysis of nine monoamines and metabolites in small amounts of peripheral murine (C57Bl/6 J) tissues

Nagler

Schriever

Angelis

et al. 2017

Biomedical Chromatography

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Monoamines, acting as hormones and neurotransmitters, play a critical role in multiple physiological processes ranging from cognitive function and mood to sympathetic nervous system activity, fight-or-flight response and glucose homeostasis. In addition to brain and blood, monoamines are abundant in several tissues, and dysfunction in their synthesis or signaling is associated with various pathological conditions. It was our goal to develop a method to detect these compounds in peripheral murine tissues. In this study, we employed a high-performance liquid chromatography method using electrochemical detection that allows not only detection of catecholamines but also a detailed analysis of nine monoamines and metabolites in murine tissues. Simple tissue extraction procedures were optimized for muscle (gastrocnemius, extensor digitorum longus and soleus), liver, pancreas and white adipose tissue in the range of weight 10-200 mg. The system allowed a limit of detection between 0.625 and 2.5 pg μL for monoamine analytes and their metabolites, including dopamine, 3,4-dihydroxyphenylacetic acid, 3-methoxytyramine, homovanillic acid, norepinephrine, epinephrine, 3-methoxy-4-hydroxyphenylglycol, serotonin and 5-hydroxyindoleacetic acid. Typical concentrations for different monoamines and their metabolization products in these tissues are presented for C57Bl/6 J mice fed a high-fat diet.

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Analytical Techniques in Neuroscience: Recent Advances in Imaging, Separation, and Electrochemical Methods

Cited by 114 publications

References 232 publications

Ultrafast neuronal imaging of dopamine dynamics with designed genetically encoded sensors

Ultrafast neuronal imaging of dopamine dynamics with designed genetically encoded sensors

Advances and Perspectives in Chemical Imaging in Cellular Environments Using Electrochemical Methods

Comprehensive analysis of nine monoamines and metabolites in small amounts of peripheral murine (C57Bl/6 J) tissues

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