Analytical Validation and Stability Indicating Studies for Simultaneous Estimation of Benidepine and Metoprolol by Strong Cation Exchange (SCX) Chromatography

A new simple, reproducible and efficient liquid chromatographic method (RP-HPLC) was developed for simultaneous estimation of thiocolchicoside and etoricoxib for tablet formulation. Formulation containing thiocolchicoside and etoricoxib is used as analgesic. Separation was achieved by Nucleosil (4.6mm I.D × 250 mm) C18 column with mobile phase consist of Acetonitrile: water (0.05% Orthophosphoric acid V/V) in the ratio 25:75 at flow rate 0.7ml . The detection was carried out at 220nm. The retention time of thiocolchicoside and etoricoxib was found to be 3.75 min and 6.13 min respectively. Linearity of THC and ETR was found to be in the range of 2-10µg/mL and 30-150µg/mL. Percent recovery obtained for THC and ETR were 99.98% and 99.69% respectively. The method was validated as per ICH guidelines.

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Strong cation exchange chromatography: a new option for concurrent quantification of saxagliptin and metformin

Manohar¹,

Kailas²

2021

J Appl Pharm Sci

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Extremely polar antidiabetic drug like metformin (MET) along with saxagliptin (SXP) often suffer from peak fronting/ tailing effects along with earlier elution with void volume in RP-HPLC. Therefore, alternative and complimentary to this RP-HPLC, the strong cation exchange (SCX) chromatography has been used for the simultaneous quantification of SXP and MEt along with stability studies. This investigation was performed on the Phenomenex Luna SCX column (100 × 2.1 mm. id, 5 μm) where both SXP and MET were eluted with ammonium formate (15 mM):MeOH (83:17 v/v) for 10 minutes with isocratic elution at 1 ml/minutes flow rate and performed at 30°C, and monitored at 230 and 260 nm wavelength where the retention time of both SXP and MET were 2.290 and 3.520 minutes, respectively. The validation studies revealed good linearity over the concentration ranging between 35.5 and 500 μg/ml for SXP and 15.5-250 μg/ml for MET with r 2 values were 1.00 and 0.999 respectively. The percentage (%) recoveries for SXP and MET were 100.37% (±1.24) and 100.39% (±1.22), respectively. Moreover, as resulted, the acid, peroxide and thermal induced stress conditions have not made any significant changes but 0.1N NaOH at 50°C induced the degradation of SXP and MET.

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Analytical Validation and Stability Indicating Studies for Simultaneous Estimation of Benidepine and Metoprolol by Strong Cation Exchange (SCX) Chromatography

Cited by 3 publications

References 3 publications

Reverse phase, ion exchange, HILIC and mix-mode chromatography for the determination of metformin and evogliptin in human plasma and pharmaceutical formulations

Reverse phase, ion exchange, HILIC and mix-mode chromatography for the determination of metformin and evogliptin in human plasma and pharmaceutical formulations

Liquid Chromatographic Method for Simultaneous estimation of Thiocolchicoside and Etoricoxib from Tablet formulation

Strong cation exchange chromatography: a new option for concurrent quantification of saxagliptin and metformin

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