Analyzing Locomotor Activity in Zebrafish Larvae Using wrMTrck

Selvaraj, Velanganni; Santhakumar, Kirankumar

doi:10.1089/zeb.2016.1395

Cited by 7 publications

(5 citation statements)

References 20 publications

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“…Using the wrMTrck software optimized for zebrafish, we inspected the locomotor patterns of the larvae treated with the different test compounds (Fig. 4 ) [ 43 , 44 ]. Visual inspection revealed that the movement patterns of larvae treated with individual compounds such as ISOX, SCOP, and CP were distinguishable from the paths of untreated control larvae (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…The settings of wrMTrck, a freely available ImageJ plugin originally developed for examining multiple behavioral parameters in the nematode Caenorhabditis elegans , have been optimized in our lab for zebrafish larvae [ 43 ]. This software was used to study the locomotor patterns of treated larvae and calculate their average speed, distance, and body bend.…”

Section: Methodsmentioning

confidence: 99%

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Novel insights on acetylcholinesterase inhibition by Convolvulus pluricaulis, scopolamine and their combination in zebrafish

Karunakaran

Thiyagaraj

Santhakumar

2022

Nat. Prod. Bioprospect.

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Acetylcholinesterase (AChE) inhibitors increase the retention of acetylcholine (ACh) in synapses. Although they alleviate cognitive deficits in Alzheimer’s disease, their limited benefits warrant investigations of plant extracts with similar properties. We studied the anti-AChE activity of Convolvulus pluricaulis (CP) in a zebrafish model of cognitive impairment induced by scopolamine (SCOP). CP is a perennial herb with anti-amnesiac and anxiolytic properties. It contains alkaloid, anthocyanin, coumarin, flavonoid, phytosterol and triterpenoid components. Isoxazole (ISOX) was used as a positive control for AChE inhibition. CP-treated 168 hpf larvae showed a similar pattern of AChE inhibition (in the myelencephalon and somites) as that of ISOX-treated larvae. CP was superior to ISOX as evidenced by the retention of avoidance response behavior in adult zebrafish. Molecular docking studies indicated that ISOX binds Ser203 of the catalytic triad on the human AChE. The active components of CP—scopoletin and kaempferol—were bound by His447 of the catalytic triad, the anionic subsite of the catalytic center, and the peripheral anionic site. This suggested the ability of CP to mediate both competitive and non-competitive modes of inhibition. Surprisingly, SCOP showed AChE inhibition in larvae, possibly mediated via the choline-binding sites. CP + SCOP induced a concentration-dependent increase in AChE inhibition and ACh depletion. Abnormal motor responses were observed with ISOX, CP, ISOX + SCOP, and CP + SCOP, indicative of undesirable effects on the peripheral cholinergic system. Our study proposes the examination of CP, SCOP, and CP + SCOP as potential AChE inhibitors for their ability to modulate cognitive deficits.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Novel insights on acetylcholinesterase inhibition by Convolvulus pluricaulis, scopolamine and their combination in zebrafish

Karunakaran

Thiyagaraj

Santhakumar

2022

Nat. Prod. Bioprospect.

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“…wrMTrck is a plugin created by Jesper Søndergaard Pedersen and his team to study how prion-like proteins spread in Caenorhabditis elegans and to analyze locomotor behavior (www.phage.dk/plugins/wrmtrck.html). 17 The plugin installation and setup are very easy, and you can read Selvaraj and Santhakumar 18 for more details. wrMTrck generates raw data, a summary, a video of the tracking analysis, and an image of the path of the animal tracking.…”

Section: Image Processing Algorithmsmentioning

confidence: 99%

A Review of Freely Available, Open-Source Software for the Automated Analysis of the Behavior of Adult Zebrafish

2019

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The analysis of behavior in animal models is an important objective in many research fields, including neuroscience, psychology, toxicology, and neuropsychopharmacology. Animal models have been used for many years, and several behavioral paradigms, such as locomotor activity, social interactions, and cognitive behavior, have been studied in animal models to correlate the behaviors with pharmacological or environmental interventions and with molecular, biochemical, and physiological findings. We reviewed the literature looking for open-source, freely available software to analyze animal behavior and found 12 freely available programs: ToxTrack, EthoWatcher, Mouse Behavior Tracker, Mouse Move, JAABA, wrMTrck, AnimalTracker, id-Tracker, Ctrax, Mousetracker, VideoHacking, and Cowlog, which were developed with different programs, work on different platforms, and have particular types of inputs and outputs and analysis capabilities. We reviewed some examples of their use, tested some of them, and provided several recommendations for the future development of programs for the automated analysis of behavior in animal models. In conclusion, we show freely available software for the automated analysis of behavior in animal models such as adult zebrafish and provide information for researchers and students looking for quick, easy-to-implement, and inexpensive behavior analysis alternatives.

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“…Laboratories without the Noldus and Maestro Edge can adapt these experiments on similar systems. Alternative cost-effective tracking and electrophysiological systems can be assembled according to Selvaraj and Santhakumar (2017) 35 and Garma et al 17 Zebrafish have multiple outstanding attributes for studies related to human neurological and neurodevelopmental disorders, including conservation of genes with human, and disease-associated phenotypes. The ability to readily perform electrophysiology allows measurements of phenotypes that include seizure propensity after chemical induction.…”

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confidence: 99%

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confidence: 99%

Noninvasive Multielectrode Array for Brain and Spinal Cord Local Field Potential Recordings from Live Zebrafish Larvae

Tomasello

Sive

2020

Zebrafish

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Zebrafish are an important and expanding experimental system for brain research. We describe a noninvasive electrophysiology technique that can be used in living larvae to measure spontaneous activity in the brain and spinal cord simultaneously. This easy-to-use method uses a commercially available multielectrode array to detect local field potential parameters, and allows for relative coordinated (network) measurements of activity. We demonstrate sensitivity of this system by measuring activity in larvae treated with the antiepileptic drug valproic acid. Valproic acid decreased larval movement and startle response, and decreased spontaneous brain activity. Spinal cord activity did not change after treatment, suggesting valproic acid primarily affects brain function. The observed differences in brain activity, but not spinal cord activity, after valproic acid treatment indicates that brain activity differences are not a secondary effect of decreased startle response and movement. We provide a step-by-step protocol for experiments presented that a novice could easily follow. This electrophysiological method will be useful to the zebrafish neuroscience community.

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Analyzing Locomotor Activity in Zebrafish Larvae Using wrMTrck

Cited by 7 publications

References 20 publications

Novel insights on acetylcholinesterase inhibition by Convolvulus pluricaulis, scopolamine and their combination in zebrafish

Novel insights on acetylcholinesterase inhibition by Convolvulus pluricaulis, scopolamine and their combination in zebrafish

A Review of Freely Available, Open-Source Software for the Automated Analysis of the Behavior of Adult Zebrafish

Noninvasive Multielectrode Array for Brain and Spinal Cord Local Field Potential Recordings from Live Zebrafish Larvae

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