Analyzing Narcissus genome compositions based on rDNA loci on chromosomes and crossing-compatibility of 16 cultivars

FISH and GISH reveal genome composition of popular Narcissus cultivars and the possible ways of their origin

“…Male fertility was referred to pollen germination according to Zeng et al (2020). When anthers were open, the fresh pollen grains were scattered on a medium overnight.…”

“…The chromosome slides were prepared from root tips according to Zeng et al (2020). When their roots were 1 − 2 cm long, four to six roots were cut off, and incubated in 0.7 mM cycloheximide (Amresco) at room temperature for 4 h in the morning, and then stored for more than one hour in a xative, containing ethanol and acetic acid (3:1, v/v).…”

Research Progress of Chromosome Doubling and 2n Gametes of Ornamental Plants

“…Chromosome count and estimation (Chromosome preparation) is the most accurate and useful method for ploidy identification, and there have been many studies that have reported chromosome count and estimation [20,27,[109][110][111]. However, some plants have minute chromosomes, making chromosome preparations difficult and experiments timeconsuming.…”

“…The use of quantitative fluorescent polymerase chain reaction (QF-PCR) analysis for quantitative genotyping also enables karyotyping. By examining the genotype of two markers per chromosome, we were able to deduce the complete karyotype [20,44]. Henry et al [115] used QF-PCR to identify euploids and aneuploids in Arabidopsis thaliana.…”

“…In addition, triploids usually have the advantage of being seedless or having fewer seeds, so from the last century to the present, breeders have used polyploid breeding to produce a large number of varieties [13][14][15][16][17]. Moreover, polyploid breeding is now widely used in ornamentals, such as triploid and tetraploid lilies [18,19], triploid and tetraploid tulips [15], triploid narcissus [20], triploid and tetraploid Paeonia [21], tetraploid Magnolia × soulangeana [7], etc.…”