Analyzing the Expression Profile of AREB/ABF and DREB/CBF Genes under Drought and Salinity Stresses in Grape (Vitis vinifera L.)

Zandkarimi, Hana; Ebadi, A.; Salami, Seyed Alireza; Alizade, H.; Baisakh, Niranjan

doi:10.1371/journal.pone.0134288

Cited by 69 publications

(51 citation statements)

References 52 publications

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“…In addition, we have observed that the gaseous treatment applied to Cardinal bunches induced the expression of other members of this transcription factor family such as CBF/DREB ( Fernandez-Caballero et al, 2012 ). The CBF/DREB proteins play a crucial role in abiotic stress-mediated gene expression, representing one of the most attractive regulons for breeding programs ( Zandkarimi et al, 2015 ) to cope with cold stress. Different studies have indeed shown the relationship between CBF/DREB expression and cold tolerance in different fruit such as tomato, peach, kiwifruit, and mango during low temperature storage in combination with different postharvest treatments ( Liang et al, 2013 ; Ma et al, 2014 ; Zhang et al, 2017 ).…”

Section: Discussionmentioning

confidence: 99%

Deciphering the Role of CBF/DREB Transcription Factors and Dehydrins in Maintaining the Quality of Table Grapes cv. Autumn Royal Treated with High CO2 Levels and Stored at 0°C

et al. 2017

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C-repeat/dehydration-responsive element binding factors (CBF/DREB) are transcription factors which play a role in improving plant cold stress resistance and recognize the DRE/CRT element in the promoter of a set of cold regulated genes. Dehydrins (DHNs) are proteins that accumulate in plants in response to cold stress, which present, in some cases, CBF/DREB recognition sequences in their promoters and are activated by members of this transcription factor family. The application of a 3-day gaseous treatment with 20 kPa CO2 at 0°C to table grapes cv. Autumn Royal maintained the quality of the bunches during postharvest storage at 0°C, reducing weight loss and rachis browning. In order to determine the role of CBF/DREB genes in the beneficial effect of the gaseous treatment by regulating DHNs, we have analyzed the gene expression pattern of three VviDREBA1s (VviDREBA1-1, VviDREBA1-6, and VviDREBA1-7) as well as three VviDHNs (VviDHN1a, VviDHN2, and VviDHN4), in both alternative splicing forms. Results showed that the differences in VviDREBA1s expression were tissue and atmosphere composition dependent, although the application of high levels of CO2 caused a greater increase of VviDREBA1-1 in the skin, VviDREBA1-6 in the pulp and VviDREBA1-7 in the skin and pulp. Likewise, the application of high levels of CO2 regulated the retention of introns in the transcripts of the dehydrins studied in the different tissues analyzed. The DHNs promoter analysis showed that VviDHN2 presented the cis-acting DRE and CRT elements, whereas VviDHN1a presented only the DRE motif. Our electrophoretic mobility shift assays (EMSA) showed that VviDREBA1-1 was the only transcription factor that had in vitro binding capacity to the CRT element of the VviDHN2 promoter region, indicating that the transcriptional regulation of VviDHN1a and VviDHN4 would be carried out by activating other independent routes of these transcription factors. Our results suggest that the application of high CO2 levels to maintain table grape quality during storage at 0°C, leads to an activation of CBF/DREBs transcription factors. Among these factors, VviDREBA1-1 seems to participate in the transcriptional activation of VviDHN2 via CRT binding, with the unspliced form of this DHN being activated by high CO2 levels in all the tissues analyzed.

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Section: Discussionmentioning

confidence: 99%

Deciphering the Role of CBF/DREB Transcription Factors and Dehydrins in Maintaining the Quality of Table Grapes cv. Autumn Royal Treated with High CO2 Levels and Stored at 0°C

et al. 2017

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“…thaliana [ 61 ]. Under saline conditions, ABF2 expression is reportedly up-regulated in grape leaves and roots in response to increasing salt concentrations [ 62 ]. Thus, the expression of salt-responsive genes may be related to the down-regulation of PP2C and up-regulation of ABF associated with ABA signal transduction.…”

Section: Discussionmentioning

confidence: 99%

Genome-wide expression analysis of salt-stressed diploid and autotetraploid Paulownia tomentosa

Zhong

Liu

et al. 2017

PLoS ONE

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Paulownia tomentosa is a fast-growing tree species with multiple uses. It is grown worldwide, but is native to China, where it is widely cultivated in saline regions. We previously confirmed that autotetraploid P. tomentosa plants are more stress-tolerant than the diploid plants. However, the molecular mechanism underlying P. tomentosa salinity tolerance has not been fully characterized. Using the complete Paulownia fortunei genome as a reference, we applied next-generation RNA-sequencing technology to analyze the effects of salt stress on diploid and autotetraploid P. tomentosa plants. We generated 175 million clean reads and identified 15,873 differentially expressed genes (DEGs) from four P. tomentosa libraries (two diploid and two autotetraploid). Functional annotations of the differentially expressed genes using the Gene Ontology and Kyoto Encyclopedia of Genes and Genomes databases revealed that plant hormone signal transduction and photosynthetic activities are vital for plant responses to high-salt conditions. We also identified several transcription factors, including members of the AP2/EREBP, bHLH, MYB, and NAC families. Quantitative real-time PCR analysis validated the expression patterns of eight differentially expressed genes. Our findings and the generated transcriptome data may help to accelerate the genetic improvement of cultivated P. tomentosa and other plant species for enhanced growth in saline soils.

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“…In signal transduction of ABA, PP2C act as negative regulator, while SnPK2 acts as positive regulator (40,41). ABA is known to acts as an endogenous messenger in plants under stressed condition and the promoter regions of many ABAresponsive genes contain a conserved cis-element that is ABA-responsive element (ABRE), PyACGTGG/TC which plays major role in ABAdependent gene expression (37).…”

Section: Aba-dependent Gene Expressionmentioning

confidence: 99%

An insight into drought stress and signal transduction of abscisic acid

Kumari

Kaur

2018

Plant Sci. Today

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The sustainable crop production is one of the major issue in the era of urbanization, industrialization, and globalization. In the environment, there are number of abiotic and biotic factors which are hampering the sustainable production of crops. The drought is one of the constraints which directly/indirectly affects the crop yield. It has various negative effects on the normal physiology and biochemistry of the plants. Therefore, researchers must have to work in the field of developing drought-tolerant crop plants to meet the food needs of the exponentially growing population of the world. The present study is the outcome of an extensive literature survey on the basic perturbations of drought to the crops, role of abscisic acid (ABA) in stressful conditions and its signal transduction.

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Analyzing the Expression Profile of AREB/ABF and DREB/CBF Genes under Drought and Salinity Stresses in Grape (Vitis vinifera L.)

Cited by 69 publications

References 52 publications

Deciphering the Role of CBF/DREB Transcription Factors and Dehydrins in Maintaining the Quality of Table Grapes cv. Autumn Royal Treated with High CO2 Levels and Stored at 0°C

Deciphering the Role of CBF/DREB Transcription Factors and Dehydrins in Maintaining the Quality of Table Grapes cv. Autumn Royal Treated with High CO2 Levels and Stored at 0°C

Genome-wide expression analysis of salt-stressed diploid and autotetraploid Paulownia tomentosa

An insight into drought stress and signal transduction of abscisic acid

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