Anaphylaxis caused by hemocyanin contained in shrimp cephalothorax

Guillén, Daiana; Fiandor, Ana; Pozo, Victoria del; Pedrosa, María; Phillips-Anglés, Elsa; Caballero, Teresa; Quirce, Santiago

doi:10.1016/j.anai.2014.09.011

Cited by 15 publications

(13 citation statements)

References 10 publications

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“…Patients with a history of anaphylaxis to shrimp but negative IgE test results underwent prick-to-prick tests using L. vannamei tail and cephalothorax. The patients tested negative for the tail but positive for the cephalothorax [9]. Immunoblot analysis using patient sera found two IgE-binding bands at 75 kDa that were identified as HC [9].…”

Section: Discussionmentioning

confidence: 97%

“…In eastern cultures (e.g., Asia-Pacific), additional body parts are also consumed (e.g., hepatopancreas and gonads) or used whole in broths and soups [8]. Research into the differential distribution of allergenicity among crustacean tissues has been poorly investigated within the current literature despite cultural differences in shellfish consumption [4,9]. The few studies that have investigated differential tissue allergenicity highlighted that patients may display allergic sensitivity after consuming shrimp cephalothorax tissues but test negative in commercial diagnostic tests using shrimp extracts that are usually based on tail muscle [9][10][11].…”

Section: Introductionmentioning

confidence: 99%

“…Research into the differential distribution of allergenicity among crustacean tissues has been poorly investigated within the current literature despite cultural differences in shellfish consumption [4,9]. The few studies that have investigated differential tissue allergenicity highlighted that patients may display allergic sensitivity after consuming shrimp cephalothorax tissues but test negative in commercial diagnostic tests using shrimp extracts that are usually based on tail muscle [9][10][11]. For improved clinical diagnosis of shellfish allergy, it is important to understand how different types of tissues differ in diversity and abundance of allergenic proteins.…”

Section: Introductionmentioning

confidence: 99%

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Allergen Diversity and Abundance in Different Tissues of the Redclaw Crayfish (Cherax quadricarinatus)

Jerry,

Karnaneedi,

Ruethers

et al. 2024

Foods

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Shellfish allergy affects ~2.5% of the global population and is a type I immune response resulting from exposure to crustacean and/or molluscan proteins. The Australian Redclaw crayfish (Cherax quadricarinatus) is a freshwater species endemic to and farmed in northern Australia and is becoming an aquaculture species of interest globally. Despite being consumed as food, allergenic proteins from redclaw have not been identified or characterised. In addition, as different body parts are often consumed, it is conceivable that redclaw tissues vary in allergenicity depending on tissue type and function. To better understand food-derived allergenicity, this study characterised allergenic proteins in various redclaw body tissues (the tail, claw, and cephalothorax) and how the stability of allergenic proteins was affected through cooking (raw vs. cooked tissues). The potential of redclaw allergens to cross-react and cause IgE-binding in patients allergic to other shellfish (i.e., shrimp) was also investigated. Raw and cooked extracts were prepared from each body part. SDS-PAGE followed by immunoblotting was performed to determine allergen-specific antibody reactivity to sarcoplasmic calcium-binding protein and hemocyanin, as well as to identify redclaw proteins binding to IgE antibodies from individual and pooled sera of shrimp-allergic patients. Liquid chromatography-mass spectrometry (LC/MS) was utilised to identify proteins and to determine the proportion within extracts. Known crustacean allergens were found in all tissues, with a variation in tissue distribution (e.g., higher levels of hemocyanin in the claw and cephalothorax than in the tail). The proportion of some allergens as a percentage of remaining heat-stable proteins increased in cooked tissues. Previously described heat-stable allergens (i.e., hemocyanin and sarcoplasmic calcium-binding protein) were found to be partially heat-labile. Immunoblotting indicated that shrimp-allergic patients cross-react to redclaw allergens. IgE-binding bands, analysed by LC/MS, identified up to 11 known shellfish allergens. The findings of this study provide fundamental knowledge into the diagnostic and therapeutic field of shellfish allergy.

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Section: Discussionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Allergen Diversity and Abundance in Different Tissues of the Redclaw Crayfish (Cherax quadricarinatus)

Jerry,

Karnaneedi,

Ruethers

et al. 2024

Foods

View full text Add to dashboard Cite

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“…Hemocyanins are considered to be among the allergens responsible for cross reactivity of HDM allergens. 20,21 Recently, Tonomura et al 22 identified another clinically important protein with a molecular weight of 40 kDa as fructose 1,6-bisphosphate aldolase in a case of food dependent, exercise induced anaphylaxis. In 2018, Kimura et al 23 identified a 43-kDa shrimp allergen as a cause of food-dependent, exercise-induced anaphylaxis.…”

Section: Discussionmentioning

confidence: 99%

Clinical utility of immunological methods based on the singleplex and multiplex ImmunoCap systems for diagnosis of shrimp allergy

et al. 2021

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Background Levels of specific IgE (sIgE) against allergen components can be assessed using multiplex assays or with highly sensitive, quantitative methods. The aim of this study was to compare the sensitivity and specificity of different immunological methods for diagnosis of shrimp allergy. Methods Twenty patients with positive skin prick tests for frozen tiger shrimp were selected for further examination. Blood samples were taken to assess concentrations of sIgE against the house dust mites Dermatophagoides pteronyssinus and D. farinae, shrimp allergen extract, allergen components Der p 1, Der p 2 and Pan a 1 (ImmunoCap), and the ImmunoCap ISAC 112 panel. Results All patients had elevated levels of sIgE against shrimp and D pteronyssinus. Eight patients were sensitized to Pen m 1, three patients were sensitized to Pen m 2, and two patients were sensitized to Pen m 4 (ISAC). ImmunoCap ISAC detected shrimp sensitization in 50% of patients. There was a strong correlation between concentrations of sIgE against Pen m1 and Der p 10 detected by ImmunoCap. Conclusions The singleplex ImmunoCap system remains the reference diagnostic method, but in the case of shrimp allergy ImmunoCap ISAC provided better insight into patient allergen profiles.

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“…Hemocyanins are copper‐containing, oxygen transport proteins mainly found in the hemolymph of invertebrate animals. Consumption of cephalothorax is common in Asian populations and could lead to higher exposure to this allergen 1058 . The 75 kD subunits create a hexameric structure for their physiological function.…”

Section: B13 – Allergy To Crustacean and Molluscsmentioning

confidence: 99%

EAACIMolecular Allergology User's Guide 2.0

Dramburg

Hilger

Santos

et al. 2023

Pediatric Allergy Immunology

121

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Since the discovery of immunoglobulin E (IgE) as a mediator of allergic diseases in 1967, our knowledge about the immunological mechanisms of IgE‐mediated allergies has remarkably increased. In addition to understanding the immune response and clinical symptoms, allergy diagnosis and management depend strongly on the precise identification of the elicitors of the IgE‐mediated allergic reaction. In the past four decades, innovations in bioscience and technology have facilitated the identification and production of well‐defined, highly pure molecules for component‐resolved diagnosis (CRD), allowing a personalized diagnosis and management of the allergic disease for individual patients. The first edition of the “EAACI Molecular Allergology User's Guide” (MAUG) in 2016 rapidly became a key reference for clinicians, scientists, and interested readers with a background in allergology, immunology, biology, and medicine. Nevertheless, the field of molecular allergology is moving fast, and after 6 years, a new EAACI Taskforce was established to provide an updated document. The Molecular Allergology User's Guide 2.0 summarizes state‐of‐the‐art information on allergen molecules, their clinical relevance, and their application in diagnostic algorithms for clinical practice. It is designed for both, clinicians and scientists, guiding health care professionals through the overwhelming list of different allergen molecules available for testing. Further, it provides diagnostic algorithms on the clinical relevance of allergenic molecules and gives an overview of their biology, the basic mechanisms of test formats, and the application of tests to measure allergen exposure.

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Anaphylaxis caused by hemocyanin contained in shrimp cephalothorax

Cited by 15 publications

References 10 publications

Allergen Diversity and Abundance in Different Tissues of the Redclaw Crayfish (Cherax quadricarinatus)

Allergen Diversity and Abundance in Different Tissues of the Redclaw Crayfish (Cherax quadricarinatus)

Clinical utility of immunological methods based on the singleplex and multiplex ImmunoCap systems for diagnosis of shrimp allergy

EAACIMolecular Allergology User's Guide 2.0

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