Anatomical and Biochemical Changes Induced by Gluconacetobacter diazotrophicus Stand Up for Arabidopsis thaliana Seedlings From Ralstonia solanacearum Infection

Rodríguez, Manuela; Tano, Josefina; Ansaldi, Nazarena; Carrau, A; Srebot, María Sol; Ferreira, Virginia; Martínez, M. Luisa; Cortadi, Adriana; Siri, María Inés; Orellano, Elena G.

doi:10.3389/fpls.2019.01618

Cited by 19 publications

(14 citation statements)

References 73 publications

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“…This fact revealed that the SA signaling pathway related to the plant's immune system plays an important role during the early association stages of the endophyte bacterium with the plant. In previous work, we did not observe lignin deposition in the xylem vessels of stems and roots, nor callose in root hairs or leaf trichomes, activated by inoculation with G. diazotrophicus in Arabidopsis sid2 mutant plants (Rodriguez et al 2019). SA can be generated through two distinct enzymatic routes from the primary metabolite chorismate.…”

Section: Introductionmentioning

confidence: 59%

“…Based on these results, inoculated Col-0 plants showed a notable boost in some of the quantitative variables measured, greater than the one observed for inoculated sid2 plants. In previous studies, low ligni cation of xylem vessels of sid2 mutant 20 dpi with G. diazotrophicus was observed compared to Col-0 inoculated plants (Rodriguez et al 2019). Accordingly, root xylem and stem vascular bundles of sid2 mutants inoculated with G. diazotrophicus 28 dpi still showed slightly lower ligni cation of xylem vessels compared to Col-0.…”

Section: Discussionmentioning

confidence: 84%

“…Each plant was carefully washed, to remove the soil and surface sterilized using ethanol 70% (v/v) for 3 minutes. After washing with sterile water, pieces of roots and stem were removed to obtain organ extracts, and CFU/ gram of organ was determined by CFU counting in plates with LGI-P supplemented with Agar, 18 g /L; Cycloheximide, 150 mg /L and Chloramphenicol, 20 mg /L; after 48 hours of incubation at 30°C (Rodriguez et al 2019). Six independent experiments were performed using 20 plant replicates for each treatment arranged in a complete randomized design.…”

Section: Plant Materials and Bacterial Strainsmentioning

confidence: 99%

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Gluconacetobacter diazotrophicus triggers ISR involving SA and JA/Et defense-related pathways to respond against Ralstonia solanacearum in Arabidopsis and optimizes the cellular redox state maintaining reduced FITNESS levels

Srebot,

Ripa,

Gallozo

et al. 2024

Plant Soil

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Background and Aims: Pest biocontrol mechanisms are bene cial effects provided by plant-PGPBEs association and remain to be elucidated. Therefore, we evaluated the possible signaling pathways involved in Arabidopsis plant defense inoculated with bene cial endophytic bacterium Gluconacetobacter diazotrophicus and phytopathogen Ralstonia pseudosolanacearum. Methods: Anatomical, physiological and genetic responses during GD-ISR (activated by inoculation with G. diazotrophicus Pal5), RP-IR (activated by inoculation with R. pseudosolanacearum GMI1000) and GDRP-ISR (both bacteria) were analyzed. Col-0, sid2 mutant and transgenic NahG were used for these studies and defense-related genes from SA and JA/Et pathways were investigated.Results: sid2 plants showed reduced colonization by G. diazotrophicus with respect to Col-0 plants and increases in xylem tissue in stems and roots of inoculated sid228 dpi plants were less signi cant than those observed in Col-0. Pr1 transcripts were augmented in inoculated Col-0 plants, while pdf 1.2 levels remained unaltered, indicating a strong role of the SA pathway during GD-ISR. Transcripts myc2 levels were elevated during GD-ISR and upon R. pseudosolanacearum infection levels of pdf 1.2 increased signi cantly, indicating that G. diazotrophicus primes the defense response against R. pseudosolanacearum. During RP-IR, pdf1.2 levels increased indicating activation of the JA pathway by R. pseudosolanacearum. G. diazotrophicusmanaged to maintain low levels of tness during GD-ISR and GDRP-ISR, leading to optimization of the stress responses network. Conclusions: G. diazotrophicus utilizes the SA pathway for effective colonization and GD-ISR activation, and primes the JA defense pathway during GD-ISR to protect Arabidopsis against the R. pseudosolanacearum during GDRP-ISR.

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Section: Introductionmentioning

confidence: 59%

Section: Discussionmentioning

confidence: 84%

Section: Plant Materials and Bacterial Strainsmentioning

confidence: 99%

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Gluconacetobacter diazotrophicus triggers ISR involving SA and JA/Et defense-related pathways to respond against Ralstonia solanacearum in Arabidopsis and optimizes the cellular redox state maintaining reduced FITNESS levels

Srebot,

Ripa,

Gallozo

et al. 2024

Plant Soil

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“…It has high biological stable activity at different temperatures and pH conditions and high bactericidal power after the first seconds of contact [ 19 ]. In this study, we elucidated the spectrum of bacteriocin action and verified its potential use against some pathogenic bacteria that attack tomato plants and other relevant crops such as peppers, potatoes, and some species of fruit trees [ 38 , 39 ]. We also evaluated Gluconacin’s effectiveness in controlling bacterial spot disease in tomato plants.…”

Section: Discussionmentioning

confidence: 99%

The Effects of Gluconacin on Bacterial Tomato Pathogens and Protection against Xanthomonas perforans, the Causal Agent of Bacterial Spot Disease

Ramos,

Olivares,

Rocha

et al. 2023

Plants

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As agricultural practices become more sustainable, adopting more sustainable practices will become even more relevant. Searching for alternatives to chemical compounds has been the focus of numerous studies, and bacteriocins are tools with intrinsic biotechnological potential for controlling plant diseases. We continued to explore the biotechnological activity of the bacteriocin Gluconacin from Gluconacetobacter diazotrophicus, PAL5 strain, by investigating this protein’s antagonism against important tomato phytopathogens and demonstrating its effectiveness in reducing bacterial spots caused by Xanthomonas perforans. In addition to this pathogen, the bacteriocin Gluconacin demonstrated bactericidal activity in vitro against Ralstonia solanacearum and Pseudomonas syringae pv. tomato, agents that cause bacterial wilt and bacterial spots, respectively. Bacterial spot control tests showed that Gluconacin reduced disease severity by more than 66%, highlighting the biotechnological value of this peptide in ecologically correct formulations.

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“…Callose deposition in leaves was found preferentially in the interveinal region of host leaves ( Gupta et al, 2017 ). Succession of structural variations is observed in Arabidopsis thaliana seedlings through callose deposition when inoculated with Gluconacetobacter diazotrophicus and protected the plant from Ralstonia solanacearum infection ( Rodriguez et al, 2019 ).…”

Section: Remodeling and Reinforcement Of Cell Wall To Cause Physical ...mentioning

confidence: 99%

Endophytes and their potential in biotic stress management and crop production

et al. 2022

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Biotic stress is caused by harmful microbes that prevent plants from growing normally and also having numerous negative effects on agriculture crops globally. Many biotic factors such as bacteria, fungi, virus, weeds, insects, and nematodes are the major constrains of stress that tends to increase the reactive oxygen species that affect the physiological and molecular functioning of plants and also led to the decrease in crop productivity. Bacterial and fungal endophytes are the solution to overcome the tasks faced with conventional farming, and these are environment friendly microbial commodities that colonize in plant tissues without causing any damage. Endophytes play an important role in host fitness, uptake of nutrients, synthesis of phytohormone and diminish the injury triggered by pathogens via antibiosis, production of lytic enzymes, secondary metabolites, and hormone activation. They are also reported to help plants in coping with biotic stress, improving crops and soil health, respectively. Therefore, usage of endophytes as biofertilizers and biocontrol agent have developed an eco-friendly substitute to destructive chemicals for plant development and also in mitigation of biotic stress. Thus, this review highlighted the potential role of endophytes as biofertilizers, biocontrol agent, and in mitigation of biotic stress for maintenance of plant development and soil health for sustainable agriculture.

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Anatomical and Biochemical Changes Induced by Gluconacetobacter diazotrophicus Stand Up for Arabidopsis thaliana Seedlings From Ralstonia solanacearum Infection

Cited by 19 publications

References 73 publications

Gluconacetobacter diazotrophicus triggers ISR involving SA and JA/Et defense-related pathways to respond against Ralstonia solanacearum in Arabidopsis and optimizes the cellular redox state maintaining reduced FITNESS levels

Gluconacetobacter diazotrophicus triggers ISR involving SA and JA/Et defense-related pathways to respond against Ralstonia solanacearum in Arabidopsis and optimizes the cellular redox state maintaining reduced FITNESS levels

The Effects of Gluconacin on Bacterial Tomato Pathogens and Protection against Xanthomonas perforans, the Causal Agent of Bacterial Spot Disease

Endophytes and their potential in biotic stress management and crop production

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