AND/R: Advanced neutron diffractometer/reflectometer for investigation of thin films and multilayers for the life sciences

Dura, Joseph A.; Pierce, D. John; Majkrzak, C. F.; Maliszewskyj, Nicholas C.; McGillivray, Duncan J.; Lösche, Mathias; O’Donovan, K. V.; Mihailescu, Mihaela; Perez-Salas, Ursula; Worcester, David L.; White, Stephen H.

doi:10.1063/1.2219744

Cited by 136 publications

(124 citation statements)

References 20 publications

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“…The neutron diffraction experiments were performed on the new Advanced Neutron Diffractometer/Reflectometer (AND/R) (Dura et al 2006) located at the NIST Center for Neutron Research, Gaithersburg, MD (http://www.ncnr.nist.gov/programs/reflect/ANDR). The sample slides were mounted vertically in the neutron beam and kept under argon in a sealed enclosure throughout the experiments.…”

Section: Neutron Diffractionmentioning

confidence: 99%

Hydration of POPC bilayers studied by 1H-PFG-MAS-NOESY and neutron diffraction

et al. 2007

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The stability of lipid bilayers is ultimately linked to the hydrophobic effect and the properties of water of hydration. Magic angle spinning (MAS) nuclear Overhauser enhancement spectroscopy (NOESY) with application of pulsed magnetic field gradients (PFG) was used to study the interaction of water with 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine bilayers in the fluid phase. NOESY cross-relaxation between water and polar groups of lipids, but also with methylene resonances of hydrophobic hydrocarbon chains, has been observed previously. This observation led to speculations that substantial amounts of water may reside in the hydrophobic core of bilayers. Here, the results of a quantitative analysis of cross-relaxation in a POPC/water mixture are reported. Coherences were selected via application of pulsed magnetic field gradients. This technique shortens acquisition times of NOESY spectra to 20 minutes and reduces t 1 -spectral noise, enabling detection of weak crosspeaks, like those between water and lipids, with higher precision than with non-gradient NOESY methods. The analysis showed that water molecules interact almost exclusively with sites of the lipid-water interface, including choline-, phosphate-, glycerol-, and carbonyl groups. The lifetime of lipid-water associations is rather short, on the order of 100 ps, at least one order of magnitude shorter than the lifetime of lipid-lipid associations. The distribution of water molecules over the lipid bilayer was measured at identical water content by neutron diffraction. Water molecules penetrate deep into the interfacial region of bilayers but water concentration in the hydrophobic core is below the detection limit of one water molecule per lipid, in excellent agreement with the cross-relaxation data.

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Section: Neutron Diffractionmentioning

confidence: 99%

Hydration of POPC bilayers studied by 1H-PFG-MAS-NOESY and neutron diffraction

et al. 2007

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“…Neutron Reflectometry-NR measurements were performed at the NG7 and NGD-MAGIK reflectometers at the NIST Center for Neutron Research (NCNR) (41). Reflectivity curves were recorded for momentum transfer values 0 Յ q z Յ 0.25 Å Ϫ1 .…”

Section: Methodsmentioning

confidence: 99%

Structural Features of Membrane-bound Glucocerebrosidase and α-Synuclein Probed by Neutron Reflectometry and Fluorescence Spectroscopy

Yap¹,

Jiang²,

Heinrich

et al. 2015

Journal of Biological Chemistry

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Background: A specific interaction exists between ␣-synuclein and glucocerebrosidase on the lipid membrane, resulting in enzyme inhibition. Results: Binding glucocerebrosidase has a profound effect on ␣-synuclein, moving roughly half of its embedded helical region above the membrane plane. Conclusion: A model is proposed with structural insights into glucocerebrosidase inhibition by ␣-synuclein. Significance: ␣-Synuclein-glucocerebrosidase interaction provides a molecular connection between Parkinson and Gaucher diseases.

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“…Neutron reflectometry (NR) has been extensively used to characterize molecularly organized surfactant and lipid layers at the sub-nano--9 -meter level. 25,26 NR measurements were performed on the Advanced Neutron Diffractometer/ Reflectometer (AND/R) 27 at the NIST Center for Neutron Research (NCNR). The resilience of the stBLMs permitted the NR characterization of the membrane at various solvent contrasts with the same physical sample.…”

Section: -Hexadecyloxymentioning

confidence: 99%

A New Lipid Anchor for Sparsely Tethered Bilayer Lipid Membranes

et al. 2009

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Mixed self-assembled monolayers (SAMs) of β-mercaptoethanol and the new synthetic lipid 1,2-di-O-palmityl-3-[ω-mercapto-nona(ethylene oxide) glycerol], FC16, were investigated for their ability to form sparsely-tethered bilayer lipid membranes (stBLMs) completed with various phospholipids. We investigated the structural and functional properties of FC16-based stBLMs and compared these to stBLMs prepared using a previously characterized synthetic lipid, 1,2-di-O-myristyl-3-[ω-mercaptohexa(ethylene oxide) glycerol] (WC14). FC16-based stBLMs show increased resistivity to ion transfer and an increase in the submembrane space of ≈ 0.5 nm. Importantly, FC16-based stBLMs formed well-defined, complete bilayers with charged phospholipids such as POPG. In these, POPG, incorporates into the outer monolayer leaflet in the same ratio as in the immersion solution, but is excluded from the inner leaflet. In all cases we investigated thus far, the area densities of the lipids within the bilayers were on average close to those in free bilayer membranes. For charged phospholipids, FC16 appears to provide a distinct advantage over WC14 for the formation of well-defined stBLMs.-2 -

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AND/R: Advanced neutron diffractometer/reflectometer for investigation of thin films and multilayers for the life sciences

Cited by 136 publications

References 20 publications

Hydration of POPC bilayers studied by 1H-PFG-MAS-NOESY and neutron diffraction

Hydration of POPC bilayers studied by 1H-PFG-MAS-NOESY and neutron diffraction

Structural Features of Membrane-bound Glucocerebrosidase and α-Synuclein Probed by Neutron Reflectometry and Fluorescence Spectroscopy

A New Lipid Anchor for Sparsely Tethered Bilayer Lipid Membranes

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