Andreas Hepp: Netzwerke der Medien. Medienkulturen und Globalisierung. Wiesbaden: Verlag für Sozialwissenschaften, 2004

Luger, Kurt

doi:10.5771/1615-634x-2005--578

Cited by 2 publications

(1 citation statement)

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“…The highly conserved nature of histone sequences has meant that results from X. laevis histones have proved broadly and successfully applicable to other species [17][18][19] . We began with the fully functional H3C110A mutant (denoted here as 'H3 WT') commonly used in biophysical studies 20 . As cysteine is not present anywhere else in this H3 variant, it provided us with a reactive handle to target for further transformation; first, through a bis-alkylation-elimination reaction to dehydroalanine (Dha), a powerful radical acceptor.…”

Section: Resultsmentioning

confidence: 99%

Intact nucleosomal context enables chromodomain reader MRG15 to distinguish H3K36me3 from -me2

Faulkner

Couturier

Josephson

et al. 2020

Preprint

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A wealth of in vivo evidence demonstrates the physiological importance of histone H3 trimethylation at lysine 36 (H3K36me3), to which chromodomain-containing proteins, such as MRG15, bind preferentially compared to their dimethyl (H3K36me2) counterparts.However, in vitro studies using isolated H3 peptides have failed to recapitulate a causal interaction. Here, we show that MRG15 can clearly discriminate between synthetic, fully intact model nucleosomes containing H3K36me2 and H3K36me3. MRG15 docking studies, along with experimental observations and nucleosome structure analysis suggest a model where the H3K36 side chain is sequestered in intact nucleosomes via a hydrogen bonding interaction with the DNA backbone, which is abrogated when the third methyl group is added to form H3K36me3. Hence, this mechanism provides a 'methyl-switch' for contextdependent reader selectivity. These results highlight the importance of such intra-chromatin interactions in understanding epigenetic regulation, a feature which is absent in commonlyused peptide or histone-only models.

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Section: Resultsmentioning

confidence: 99%