Androgen metabolism in the skin of the rainbow trout (Oncorhynchus mykiss)

Latz, Martin; Reinboth, Rudolf

doi:10.1007/bf00004576

Cited by 9 publications

(2 citation statements)

References 17 publications

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“…In another species, the urohaze-goby (Glossogobius olivaceus), DHTS was the main product of in vitro steroidogenesis in testes tissue (Asahina et al 1985). In addition, enzymatic activity of 5-α reductase, the enzyme that converts TS to DHTS, has been demonstrated in different tissues, including the brain and pituitary, in several teleost species (Pasmanik & Callard 1988a, Latz & Reinboth 1993, Margiotta-Casaluci et al 2013, so it is likely that DHTS plays a biological role not yet described in teleosts. In the present study, DHTS stimulated fshb expression in cells from mature cod, which, to our knowledge, is the first time effects on fshb expression from a non-aromatizable androgen have been demonstrated in vitro in a multibatch spawner.…”

Section: Gonadotropin Expressionmentioning

confidence: 99%

Sex steroids differentially regulate fshb, lhb and gnrhr expression in Atlantic cod (Gadus morhua)

et al. 2017

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Depending on the stage of gonad maturation, as well as other factors, gonadal steroids can exert either a positive or negative feedback at the brain and pituitary level. While this has been demonstrated in many teleost species, little is known about the nature of steroid feedback in Gadiform fish. Using an optimized model system of the Atlantic cod pituitary, the present study investigated the potential effects of two physiologically relevant doses of estradiol, testosterone (TS) or dihydrotestosterone (DHTS) on cell viability and gene expression of gonadotropin subunits (/) and two suggested reproduction-relevant gonadotropin-releasing hormone receptors (/) during three stages of sexual maturity. In general, all steroids stimulated cell viability in terms of metabolic activity and membrane integrity. Furthermore, all steroids affected expression, with the effect depending on both the specific steroid, dose and maturity status. Conversely, only DHTS exposure affected levels, and this occurred only during the spawning season. Using single-cell qPCR, co-transcription of and was confirmed to both and expressing gonadotropes, with being the most prominently expressed isoform. While steroid exposure had no effect on expression, all steroids affected transcript levels in at least one maturity stage. These and previous results from our group point to Gnrhr2a as the main modulator of gonadotropin regulation in cod and that regulation of its gene expression level might function as a direct mechanism for steroid feedback at the pituitary level.

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Section: Gonadotropin Expressionmentioning

confidence: 99%

Sex steroids differentially regulate fshb, lhb and gnrhr expression in Atlantic cod (Gadus morhua)

et al. 2017

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“…Other studies showed that the activity of 5AR in goldfish (Carassius auratus) was high in nonreproductive tissues such as the liver, brain and pituitary tissues, and it was reported that the expression pattern of SRD5A2 in toadfish (Opsanus tau) was significantly higher in the liver than in the gonad, in contrast to that in humans [36,37]. In the case of rainbow trout (Oncorhynchus mykiss), SRD5A activity was confirmed in the skin of males and females [38,39]. Although we did not measure the 5AR activity in whole tissue cells, our results demonstrated that fish cell lines are more sensitive to testosterone than human cell lines.…”

Section: Discussionmentioning

confidence: 96%

Development of a Liquid Chromatography/Mass Spectrometry-Based Inhibition Assay for the Screening of Steroid 5-α Reductase in Human and Fish Cell Lines

et al. 2021

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Steroid 5-α reductase (5AR) is responsible for the reduction of steroids to 5-α reduced metabolites, such as the reduction of testosterone to 5-α dihydrotestosterone (DHT). A new adverse outcome pathway (AOP) for 5AR inhibition to reduce female reproduction in fish (AOP 289) is under development to clarify the antiestrogenic effects of 5AR inhibitors in female fish. A sensitive method for the DHT analysis using chemical derivatization and liquid chromatography–tandem mass spectrometry was developed. A cell-based 5AR inhibition assay that utilizes human cell lines, a transient overexpression system, and fish cell lines was developed. The measured IC50 values of two well-known 5AR inhibitors, finasteride and dutasteride, were comparable in the different systems. However, the IC50 of dutasteride in the fish cell lines was lower than that in the human cell lines. Finasteride showed a higher IC50 against the RTG-2 cell line. These results demonstrated that 5ARs inhibition could differ in terms of structural characteristics among species. The assay has high sensitivity and reproducibility and is suitable for the application in 5AR inhibition screening for various endocrine disruption chemicals (EDCs). Future studies will continue to evaluate the quantitative inhibition of 5AR by EDCs to compare the endocrine-disrupting pathway in different species.

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