Andrographis paniculata can modulate the ratio of Treg to Th17 cells in atherosclerotic rats

Abstract

s u m m a r yThe purpose of this study is to analyze the effect of Andrographis paniculata (Burm F) in the modulation of naive T cell differentiation and its related cytokines in the rat model of atherosclerosis. Twenty-five male rats were assigned to five study groups, consisting of the control group (normal diet), the atherosclerosis rat group (atherogenic diet), and the atherosclerotic rat group receiving A. paniculata (Burm F) extracts in three doses, there were 0.6 mg/kgBBW (Ather þ AP1 group), 1.2 mg/kgBBW (Ather þ AP2 group) and 2.4 mg/kgBBW (Ather þ AP3 group). The numbers of Th17 and Treg cells were measured using flow cytometry. The IL-17 and IL-6 levels were analyzed using enzyme-linked immunosorbent assay technique. Th17 cell level increased significantly in the atherosclerosis group compared to the control group (p > 0.05). The A. paniculata extract in the 0.6 mg/kgBBW and 2.4 mg/kgBBW doses could decrease the Th17 cell count significantly compared to the atherosclerosis group (p < 0.05), despite did not achieve a normal value similar to the control group (p < 0.05). The A. paniculata extract in the 1.2 mg/kgBBW dose significantly increased Treg cell count compared to the other groups (p < 0.05). The ratio of Treg cells to Th17 cells dropped significantly in the atherosclerosis group to the control group (p > 0.05). A. paniculata extract of

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“…Aterosklerosis merupakan kondisi perubahan patologi vaskular yang ditandai dengan peradangan dan plak didinding arteri [1]. Aterosklerosis adalah penyakit arteri progresif yang nantinya dapat berkembang menjadi penyakit jantung dan stroke.…”

The roles of immune cells in atherosclerotic calcification

“…Th17 cells, which are pro-inflammatory mediators that produce IL-17, IL-22 and IL-23, 114 mediate proatherogenic progression. 115,116 Studies have shown that Th17 cell levels increase significantly in atherosclerotic rats, 117 and IL-17 expression (a marker of Th17 cells) is upregulated in patients with atherosclerosis. 118 This effect may be associated with the role of Th17 cells in stabilizing plaques through induced fibrosis.…”