Andrographolide-loaded nanoparticles for brain delivery: Formulation, characterisation and in vitro permeability using hCMEC/D3 cell line

Guccione, Clizia; Oufir, Mouhssin; Piazzini, Vieri; Eigenmann, Daniela Elisabeth; Jähne, Evelyn A.; Zabela, Volha; Faleschini, Maria Teresa; Bergonzi, Maria Camilla; Smieško, Martin; Hamburger, Matthias

doi:10.1016/j.ejpb.2017.06.018

Cited by 39 publications

(29 citation statements)

References 31 publications

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“…P app of NaF was 8.27 ± 1.81 × 10 −6 cm/s, in agreement with the literature values [ 11 , 12 , 19 ]. This value remained constant during the permeability assay, and demonstrates the confluence of the monolayer and assesses the tight junction integrity.…”

Section: Resultssupporting

confidence: 92%

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Stealth and Cationic Nanoliposomes as Drug Delivery Systems to Increase Andrographolide BBB Permeability

et al. 2018

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(1) Background: Andrographolide (AG) is a natural compound effective for the treatment of inflammation-mediated neurodegenerative disorders. The aim of this investigation was the preparation of liposomes to enhance the penetration into the brain of AG, by modifying the surface of the liposomes by adding Tween 80 (LPs-AG) alone or in combination with Didecyldimethylammonium bromide (DDAB) (CLPs-AG). (2) Methods: LPs-AG and CLPs-AG were physically and chemically characterized. The ability of liposomes to increase the permeability of AG was evaluated by artificial membranes (PAMPA) and hCMEC/D3 cells. (3) Results: Based on obtained results in terms of size, homogeneity, ζ-potential and EE%. both liposomes are suitable for parenteral administration. The systems showed excellent stability during a month of storage as suspensions or freeze-dried products. Glucose resulted the best cryoprotectant agent. PAMPA and hCMEC/D3 transport studies revealed that LPs-AG and CLPs-AG increased the permeability of AG, about an order of magnitude, compared to free AG without alterations in cell viability. The caveolae-mediated endocytosis resulted the main mechanism of up-take for both formulations. The presence of positive charge increased the cellular internalization of nanoparticles. (4) Conclusions: This study shows that developed liposomes might be ideal candidates for brain delivery of AG.

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Section: Resultssupporting

confidence: 92%

“…In a previous research studies, the authors developed solid lipid nanoparticles [ 11 ] and polymeric nanoparticles [ 12 ] to deliver the andrographolide (AG), a natural compound, through the central nervous system and ameliorate its biopharmaceutical characteristics.…”

Section: Introductionmentioning

confidence: 99%

Stealth and Cationic Nanoliposomes as Drug Delivery Systems to Increase Andrographolide BBB Permeability

et al. 2018

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“…Other studies have focused on the use of andrographolide loaded into human serum albumin-based nanoparticles and polyethylcyanoacrylate nanoparticles for the treatment of inflammation related to neurodegenerative diseases. Results showed a slightly increased permeability for the human serum albumin nanoparticles, while the polyethylcyanoacrylate nanoparticles reversibly disrupted the integrity of the cell monolayer utilized for the in vitro experiment [61]. The delivery of docetaxel for the treatment of brain metastasis has been achieved through the development of penetrating amphiphilic polymer-lipid nanoparticles system.…”

Section: Nanotechnology Approaches For Crossing the Blood-brain Bamentioning

confidence: 99%

Blood-Brain Delivery Methods Using Nanotechnology

et al. 2018

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Pathologies of the brain, of which brain cancer, Alzheimer’s disease, Parkinson’s disease, stroke, and multiple sclerosis, are some of the most prevalent, and that presently are poorly treated due to the difficulties associated with drug development, administration, and targeting to the brain. The existence of the blood-brain barrier, a selective permeability system which acts as a local gateway against circulating foreign substances, represents the key challenge for the delivery of therapeutic agents to the brain. However, the development of nanotechnology-based approaches for brain delivery, such as nanoparticles, liposomes, dendrimers, micelles, and carbon nanotubes, might be the solution for improved brain therapies.

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“…Nanoparticle formulations represent a smart approach to increase stability and/or solubility and as a consequence bioavailability of natural products [23,26,29,[42][43][44] but also to enhance permeation and crossing biological barriers including the blood-brain barrier [45][46][47].…”

Section: Discussionmentioning

confidence: 99%

Development and Percutaneous Permeation Study of Escinosomes, Escin-Based Nanovesicles Loaded with Berberine Chloride

et al. 2019

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Escin is a natural saponin, clinically used for the anti-edematous and anti-inflammatory effects. The aim of the study was to explore the possibility of converting escin into vesicle bilayer-forming component. The hyaluronidase inhibition activity of escin was evaluated after its formulation in escinosomes. Berberine chloride, a natural quaternary isoquinoline alkaloid isolated from several medicinal plants that is traditionally used for various skin conditions was loaded in the vesicles. The developed nanovesicles were characterized in terms of diameter, polydispersity, ζ-potential, deformability, recovery, encapsulation efficiency, stability, and release kinetics. Nanovesicle permeation properties through artificial membranes and rabbit ear skin were investigated using skin-PAMPATM and Franz cells were also evaluated. Escinosomes, made of phosphatidylcholine and escin, were loaded with berberine chloride. These nanovesicles displayed the best characteristics for skin application, particularly optimal polydispersity (0.17) and deformability, high negative ζ-potential value, great encapsulation efficiency (about 67%), high stability, and the best release properties of berberine chloride (about 75% after 24 h). In conclusion, escinosomes seem to be new vesicular carriers, capable to maintain escin properties such as hyaluronidase inhibition activity, and able to load other active molecules such as berberine chloride, in order to enhance or expand the activity of the loaded drug.

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Andrographolide-loaded nanoparticles for brain delivery: Formulation, characterisation and in vitro permeability using hCMEC/D3 cell line

Cited by 39 publications

References 31 publications

Stealth and Cationic Nanoliposomes as Drug Delivery Systems to Increase Andrographolide BBB Permeability

Stealth and Cationic Nanoliposomes as Drug Delivery Systems to Increase Andrographolide BBB Permeability

Blood-Brain Delivery Methods Using Nanotechnology

Development and Percutaneous Permeation Study of Escinosomes, Escin-Based Nanovesicles Loaded with Berberine Chloride

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