Anemoside B4 prevents chronic obstructive pulmonary disease through alleviating cigarette smoke-induced inflammatory response and airway epithelial hyperplasia

Ma, Huimiao; Zhou, Ziye; Chen, Lan‐Ying; Lingling, Wang; Mei, Qi

doi:10.1016/j.phymed.2022.154431

Cited by 13 publications

(4 citation statements)

References 33 publications

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“…In this study, the viability of cells treated with 5% and 25% CSE was lower than that of control cells. Wang et al treated macrophages with 1%, 2.5%, 5%, 7.5%, and 10% CSE for 24 h and found that cell viability began to decrease when the CSE concentration was ≥7.5% [ 29 ], whereas another study revealed that 10% CSE reduced cell viability [ 30 ]. This may be related to the different CSE preparation methods employed and cell lines selected in the experiments.…”

Section: Discussionmentioning

confidence: 99%

Bilirubin regulates cell death type by alleviating macrophage mitochondrial dysfunction caused by cigarette smoke extract

Wei,

Tian,

Wei

et al. 2024

Redox Report

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Objectives To explore the effects and mechanisms of bilirubin on mitochondrial function and type of macrophage cell death after exposure to cigarette smoke extract (CSE). Methods RAW264.7 macrophages were treated with different concentrations of CSE and bilirubin solutions and divided into four groups: control, CSE, bilirubin, and bilirubin + CSE groups. The necrotic and apoptotic states of the macrophages were determined using an Annexin V-fluorescein 5-isothiocyanate/propidium iodide (FITC/PI) staining kit. Cytoplasmic NOD-like receptor family, pyrin domain containing 3 (NLRP3) expression in macrophages was detected by immunofluorescence and the levels of IL-1β and IL-18 in the supernatants of culture medium were detected by enzyme linked immunosorbent assay (ELISA) test. A JC-1 mitochondrial membrane potential detection kit was used to assess mitochondrial membrane damage and the adenosine triphosphate (ATP) assay kit was used to determine intracellular ATP levels. After the macrophages were stained with reactive oxygen species (ROS) specific dye, 2',7'-Dichlorodihydrofluorescein diacetate (DCFH-DA), the fluorescence intensity and proportion of ROS-positive macrophages were measured using flow cytometry. Results We observed that compared with those of 0 μM (control group), concentrations of 5, 10, or 20 μΜ bilirubin significantly decreased cell viability, which was increased by bilirubin exposure below 1 μM. The effect of CSE on macrophage viability was concentration- and time-dependent. Bilirubin of 0.2 μM could alleviate the inhibition of macrophage viability caused by 5% CSE. In addition, bilirubin intervention could reduce the occurrence of necrosis and pyroptosis to a certain extent. Conclusions CSE could cause mitochondrial dysfunction in macrophages, as demonstrated by a decrease in mitochondrial membrane potential and intracellular ATP levels and an increase in ROS production, while bilirubin could relieve mitochondrial dysfunction caused by CSE.

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Section: Discussionmentioning

confidence: 99%

Bilirubin regulates cell death type by alleviating macrophage mitochondrial dysfunction caused by cigarette smoke extract

Wei,

Tian,

Wei

et al. 2024

Redox Report

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“…Anemoside B4 has shown significant promise in attenuating the increased levels of inflammatory markers, including IL-1β, TGF-β, IL-8, and TNF-α, in human bronchial epithelial cells exposed to cigarette smoke extract (CSE). This is achieved by inhibiting the MAPK/AP-1/TGF-β signaling pathway, thereby preventing chronic pulmonary obstructive disease induced by cigarette smoke ( Ma et al, 2022 ). Isoliquiritigenin effectively reduces inflammation induced by Mycobacterium tuberculosis (Mtb) through the Notch1/NF-κB and MAPK signaling pathways ( Sun et al, 2022 ).…”

Section: Treatment Of Other Lung-related Diseases With Traditional Ch...mentioning

confidence: 99%

The mechanism and application of traditional Chinese medicine extracts in the treatment of lung cancer and other lung-related diseases

He,

Wang,

Han

et al. 2023

Front. Pharmacol.

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Lung cancer stands as one of the most prevalent malignancies worldwide, bearing the highest morbidity and mortality rates among all malignant tumors. The treatment of lung cancer primarily encompasses surgical procedures, radiotherapy, and chemotherapy, which are fraught with significant side effects, unfavorable prognoses, and a heightened risk of metastasis and relapse. Although targeted therapy and immunotherapy have gradually gained prominence in lung cancer treatment, diversifying the array of available methods, the overall recovery and survival rates for lung cancer patients remain suboptimal. Presently, with a holistic approach and a focus on syndrome differentiation and treatment, Traditional Chinese Medicine (TCM) has emerged as a pivotal player in the prognosis of cancer patients. TCM possesses characteristics such as targeting multiple aspects, addressing a wide range of concerns, and minimizing toxic side effects. Research demonstrates that Traditional Chinese Medicine can significantly contribute to the treatment or serve as an adjunct to chemotherapy for lung cancer and other lung-related diseases. This is achieved through mechanisms like inhibiting tumor cell proliferation, inducing tumor cell apoptosis, suppressing tumor angiogenesis, influencing the cellular microenvironment, regulating immune system function, impacting signal transduction pathways, and reversing multidrug resistance in tumor cells. In this article, we offer an overview of the advancements in research concerning Traditional Chinese Medicine extracts for the treatment or adjunctive chemotherapy of lung cancer and other lung-related conditions. Furthermore, we delve into the challenges that Traditional Chinese Medicine extracts face in lung cancer treatment, laying the foundation for the development of diagnostic, prognostic, and therapeutic targets.

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“…This treatment strategy focuses on leveraging multiple anti-cancer mechanisms to enhance the inhibition of carcinogenesis, mitigate drug resistance, and alleviate chemotherapy-related side effects [ 24 ]. Pulsatilla chinensis (Bunge) Regel, a well-known traditional Chinese medicine, is recognized for its “blood-cooling” and detoxification properties [ 25 ]. Among its significant active compounds is 23-hydroxybetulinic acid (23-HBA), a pentacyclic triterpenoid extracted from the root of P. chinensis .…”

Section: Introductionmentioning

confidence: 99%

23-Hydroxybetulinic acid attenuates 5-fluorouracil resistance of colorectal cancer by modulating M2 macrophage polarization via STAT6 signaling

Fan,

Cui,

Chen

et al. 2024

Cancer Immunol Immunother

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Macrophage polarization is closely associated with the inflammatory processes involved in the development and chemoresistance of colorectal cancer (CRC). M2 macrophages, the predominant subtype of tumor-associated macrophages (TAMs) in a wide variety of malignancies, have been demonstrated to promote the resistance of CRC to multiple chemotherapeutic drugs, such as 5-fluorouracil (5-FU). In our study, we investigated the potential of 23-Hydroxybetulinic Acid (23-HBA), a significant active component of Pulsatilla chinensis (P. chinensis), to inhibit the polarization of M2 macrophages induced by IL-4. Our results showed that 23-HBA reduced the expression of M2 specific marker CD206, while downregulating the mRNA levels of M2 related genes (CD206, Arg1, IL-10, and CCL2). Additionally, 23-HBA effectively attenuated the inhibitory effects of the conditioned medium from M2 macrophages on apoptosis in colorectal cancer SW480 cells. Mechanistically, 23-HBA prevented the phosphorylation and nuclear translocation of the STAT6 protein, resulting in the inhibition of IL-10 release in M2 macrophages. Moreover, it interfered with the activation of the IL-10/STAT3/Bcl-2 signaling pathway in SW480 cells, ultimately reducing M2 macrophage-induced resistance to 5-FU. Importantly, depleting STAT6 expression in macrophages abolished the suppressive effect of 23-HBA on M2 macrophage polarization, while also eliminating its ability to decrease M2 macrophage-induced 5-FU resistance in cancer cells. Furthermore, 23-HBA significantly diminished the proportion of M2 macrophages in the tumor tissues of colorectal cancer mice, simultaneously enhancing the anti-cancer efficacy of 5-FU. The findings presented in this study highlight the capacity of 23-HBA to inhibit M2 macrophage polarization, a process that contributes to reduced 5-FU resistance in colorectal cancer.

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Anemoside B4 prevents chronic obstructive pulmonary disease through alleviating cigarette smoke-induced inflammatory response and airway epithelial hyperplasia

Cited by 13 publications

References 33 publications

Bilirubin regulates cell death type by alleviating macrophage mitochondrial dysfunction caused by cigarette smoke extract

Bilirubin regulates cell death type by alleviating macrophage mitochondrial dysfunction caused by cigarette smoke extract

The mechanism and application of traditional Chinese medicine extracts in the treatment of lung cancer and other lung-related diseases

23-Hydroxybetulinic acid attenuates 5-fluorouracil resistance of colorectal cancer by modulating M2 macrophage polarization via STAT6 signaling

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