Aneuploidy Screening using Next Generation Sequencing

Cinnioğlu, Cengiz; Kayalı, Refik; Darvin, Tristan; Akinwole, Adedoyin; Jakubowska, Milena; Harton, G.

doi:10.1007/978-1-4939-8889-1_6

Cited by 4 publications

(3 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…3,4 Preimplantation genetic testing for aneuploidy (PGT-A) has been known as the "third-generation" technique used in the area of ART to select the euploid embryo for transfer. 5 It is generally accepted that cleavage-stage embryo biopsy significantly impairs embryonic reproductive potential, while trophectoderm biopsy has no meaningful impact on the implantation and delivery rates. 6,7 Therefore, most biopsies are currently performed on the trophectoderm of blastocysts.…”

Section: Introductionmentioning

confidence: 99%

Double vitrification–warming cycles, coupled with blastocyst biopsy, impair live birth but do not affect neonatal outcomes

Jia

et al. 2022

Intl J Gynecology & Obste

View full text Add to dashboard Cite

Objective To identify whether the transfer of blastocysts that have been vitrified, thawed, biopsied, revitrified, and subsequently rethawed affects clinical outcome and neonatal outcome. Methods A retrospective study was conducted in a single assisted reproduction technology center from September 2016 to March 2021. Women undergoing single frozen euploid blastocysts transfer were stratified into two groups based on number of vitrification‐thawing cycles: single vitrification coupled with single biopsy (group A, n = 177) and double vitrification coupled with single biopsy (group B, n = 30). Pregnancy and perinatal outcomes of the two groups were compared. Results Clinical pregnancy rates were similar between the two groups. Group B was associated with an increased likelihood of live birth when compared with group A by different multivariable analysis models (model 1: odds ratio, 0.42 [95% confidence interval, 0.18–0.97], P = 0.041; model 2: odds ratio, 0.38 [95% confidence interval, 0.16–0.92], P = 0.033). No major obstetrical complication was reported in the two groups and only one malformation live birth was reported in group A. Conclusion The procedure of double vitrification‐warming cycles, coupled with single biopsy, increases pregnancy loss and ultimately diminishes live birth but does not affect perinatal outcome. Future studies with a larger sample size would help to validate the results.

show abstract

Section: Introductionmentioning

confidence: 99%

Double vitrification–warming cycles, coupled with blastocyst biopsy, impair live birth but do not affect neonatal outcomes

Jia

et al. 2022

Intl J Gynecology & Obste

View full text Add to dashboard Cite

show abstract

“…NGS -дає можливість визначити анеуплоїдії, незбалансовані транслокації, часткові анеуплоїдії, триплоїдії, мозаїчні ембріони (з 20% і більше аномальних клітин) [5,8,45]. Частота помилки 1-2%.…”

unclassified

“…SNPs -можливо визначити анеуплоїдії, незбалансовані транслокації, часткові анеуплоїдії, триплоїдії, уніпатернальні дисомії, але пошук мозаїцизмів обмежений. Діагностика анеуплоїдій при близькоспоріднених шлюбах неможлива [5,42]. Частота помилки 2-4%.…”

unclassified

Does every family couple that being treated with assisted reproductive technologies need to use preimplantation genetic testing for aneuploidy (PGT-A)?

Lyovkina

Derii

Kuzmenko

2019

Rep. of Vinnytsia Nation. Med. Univ.

View full text Add to dashboard Cite

The increase in the frequency of using assisted reproductive technologies (ART) dictates the need for pre-implantation diagnosis of embryos to determine which of them are euploid and recommended for embryo transfer (ET), since the main cause of implantation failures when using in vitro fertilization (IVF) is ET aneuploid or mosaic ETs or mosaic imitations that use in vitro fertilization (IVF) is ET aneuploid or mosaic imitative failures when using in vitro fertilization (IVF) is ET aneuploid or mosaic imitations. . For this purpose, the PGT-A technology was created, since the appearance of which and its further development there have been discussions about the feasibility of using PGT-A for each pair, which turned to the use of ART. The purpose of this literature review is to describe the current status of PGT-A and determine the prospects for its widespread adoption in the practice of reproductive medicine. A literature search was carried out in the PubMed and Cochrane databases for the last 10 years. An analysis of the literature has shown that IVF with PGT-A technology has significant advantages over traditional IVF; The technology has a number of technical and financial limitations, which makes it difficult to massively introduce technology into the practice of reproductive medicine, therefore at this stage there should be clear indications for using IVF with PGT-A.

show abstract

Current Status and Recent Advances in Preimplantation Genetic Testing for Structural Rearrangements

Zhang

Chen

Sun

et al. 2020

Reproductive and Developmental Medicine

View full text Add to dashboard Cite

Aneuploidy Screening using Next Generation Sequencing

Abstract: Chromosomal aneuploidy is recognized to be a significant contributing factor in implantation failure and spontaneous miscarriage Hellani et al.

Cited by 4 publications

References 12 publications

Double vitrification–warming cycles, coupled with blastocyst biopsy, impair live birth but do not affect neonatal outcomes

Double vitrification–warming cycles, coupled with blastocyst biopsy, impair live birth but do not affect neonatal outcomes

Does every family couple that being treated with assisted reproductive technologies need to use preimplantation genetic testing for aneuploidy (PGT-A)?

Current Status and Recent Advances in Preimplantation Genetic Testing for Structural Rearrangements

Contact Info

Product

Resources

About