Aneurinibacillus soli sp. nov., isolated from mountain soil

Abstract

A novel bacterial strain designated CB4 T was isolated from soil from the Hallasan, Jeju, Korea. Strain CB4 T was found to be strictly aerobic, Gram-stain-positive, rod-shaped, motile and formed creamy greyish colonies on nutrient agar. The major fatty acids were identified as iso-C 15 : 0 and iso-C 16 : 0 , and the predominant isoprenoid quinone as MK-7. The cell-wall peptidoglycan contained glycine and alanine as the diagnostic amino acids and phosphatidyl-Nmethylethanolamine, phosphatidylethanolamine, diphosphatidylglycerol and an unidentified aminophospholipid as the polar lipids. The genomic DNA G+C content of strain CB4 T was 46.5 mol%. Phylogenetic analysis, based on 16S rRNA gene sequence similarities, showed that strain CB4 T forms a deep branch within the genus Aneurinibacillus, sharing the highest level of sequence homology with Aneurinibacillus aneurinilyticus DSM 5562 T (96.5 %). On the basis of the phenotypic, chemotaxonomic and phylogenetic characteristics, strain CB4 T is considered to represent a novel species within the genus Aneurinibacillus, for which the name Aneurinibacillus soli sp. nov. is proposed. The type strain is CB4 T (5KCTC 33505 T 5CECT 8566 T ). An emended description of the genus Aneurinibacillus is also proposed.Bacillus aneurinolyticus represented first as a new thiamindecomposing bacterium (Aoyama, 1952) and has been revived as described by Shida et al. (1994). The genus Aneurinibacillus was proposed as novel genus arising from the reclassification of the Bacillus aneurinilyticus and related species of the genus Bacillus (Shida et al., 1996). At the time of writing, the genus Aneurinibacillus is composed of five species: Aneurinibacillus aneurinilyticus (Shida et al., 1994(Shida et al., , 1996Heyndrickx et al., 1997) the major cellular fatty acids are iso-C 15 : 0 and iso-C 16 : 0 ; and the DNA G+C content ranges from 42.9 to 46.7 mol% (Shida et al., 1994, Goto et al., 2004.Hallasan is the tallest mountain (1,950 m above sea level) in South Korea and is located on the southernmost island of the Korean Peninsula. The soil of Hallasan, a young volcanic mountain of the fourth Cenozoic era, is derived from volcanic ash. Hallasan has special scientific importance due to its diverse vegetation. Strain CB4 T was isolated during an investigation into the bacterial diversity of mountain soil of the Hallasan. On the basis of the phenotypic and chemotaxonomic characterization and 16S rRNA gene sequence analysis we show that the new bacterium isolated from mountain soil belongs to the genus Aneurinibacillus and represents a novel species.A soil sample was collected from Hallasan, Jeju, Republic of Korea. A portion of 1 g of soil was diluted serially in sterile distilled water and 100 ml of each dilution was spread on R2A agar (BD). Plates were incubated aerobically at 25 u C for 4 weeks. A strain, designated CB4 T , was isolated from a single colony and purified by subculturing on the same medium. The bacterial culture was stored at 270 u C in a water suspension supplemented with 20 % (...

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Aneurinibacillus uraniidurans sp. nov., a uranium-resistant bacterium isolated from uranium-contaminated soil

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Genome-Driven Discovery of Enzymes with Industrial Implications from the Genus Aneurinibacillus

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