Angiogenesis Assays Using Chick Chorioallantoic Membrane

West, David C.; Thompson, W. D.; Sells, Paula G.; Burbridge, Mike F.

doi:10.1385/1-59259-143-4:107

Cited by 54 publications

(56 citation statements)

References 30 publications

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“…10-12 days for mouse embryos [33] and 2-5 weeks in human embryos [34], the system is timeefficient. Since the use of chick embryos in stages of up to 10 days of incubation does not evoke severe ethical issues [35] its usage for the study of glucose-induced malformations during embryonic development would be an appropriate alternative to other animal experiments.…”

Section: Discussionmentioning

confidence: 99%

Shell-Less Chick Embryo Culture as an Alternative in vitro Model to Investigate Glucose-Induced Malformations in Mammalian Embryos

Datar¹,

Bhonde²

2005

Rev Diabetic Stud

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■ AbstractWe have developed a simple shell-less chick embryo culture system to study glucose-induced malformations. This system involves the culturing of chick embryos from the second day to the fifth day of incubation, with associated yolk and thick and thin albumen outside the egg shell. The system allows the observation of embryonic development of chicks in a glass bowl. Developing embryos at 24 h, 48 h and 72 h incubation, corresponding to the Hamberger Hamilton (HH) stages from 7 to 21, were treated with two concentrations of glucose (50 mM and 100 mM) for 24 h. Glucose treatment resulted in a mortality rate of over 70% in younger embryos. Furthermore, a variety of malformations such as retarded growth, abnormal heart development, macrosomia, exencephaly, etc. were observed in older embryos, which were similar to those reported in mammalian embryos as a consequence of diabetic pregnancy. The glucose-induced malformations were found to be concentration-and stage-dependent, thus emphasizing the roles of the degree of hyperglycemia and the stage of embryonic development in diabetic growth anomalies. Here we demonstrate for the first time that the present system can be used (i) for experiments at early stages of chick embryo development and (ii) for assessing the effects of acute glucose toxicity similar to those reported for mammalian embryos in a hyperglycemic environment.

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Section: Discussionmentioning

confidence: 99%

Shell-Less Chick Embryo Culture as an Alternative in vitro Model to Investigate Glucose-Induced Malformations in Mammalian Embryos

Datar¹,

Bhonde²

2005

Rev Diabetic Stud

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“…Therefore, the possibility cannot be ruled out that the small disc itself contributes in some way to the angiogenic responsiveness seen after the application of VEGF on Day 13. -For instructive technical details, see [46,52].…”

Section: De Novo Vegf-mediated Angiogenesismentioning

confidence: 99%

In vivo models of angiogenesis

2006

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“…The antiangiogenic effect of the extracts was screened in vivo using a modified fertilised chicken embryos assay [12]. Briefly, 5-day old fertilised eggs were obtained from a local hatchery and 5 mL albumin was withdrawn.…”

Section: In Vivo Chorioallantoic Membrane (Cam) Assaymentioning

confidence: 99%