2015
DOI: 10.4236/abcr.2015.44011
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Angiogenesis Factors Associated with New Breast Cancer Cell Line AMJ13 Cultured <i>in Vitro</i>

Abstract: Background: AMJ13 is a new breast cancer cell line that has been established from a 70-year-old Iraqi woman with a histological diagnosis of infiltrating ductal carcinoma. It is the first for an Iraqi population. In breast cancer, angiogenesis provides the tumor tissue, which is rapidly proliferated with oxygen and nutrients, removes wastes and increases the opportunity of cancer cells to invade other organs. Methods: The AMJ13 breast cancer cell line was represented at three different passages and incubated f… Show more

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Cited by 4 publications
(2 citation statements)
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“…Te AMJ13 cell line, derived from Iraqi patient with breast cancer cells, exhibits a lack of estrogen and progesterone receptors [24]. Tis cell line has been widely utilized in our laboratory as well as by other researchers [25][26][27][28]. Human breast cancer cell line estrogen, progesterone receptors positive (MCF-7), were provided by the Experimental Terapy Department, ICCMGR, Baghdad, Iraq.…”
Section: Cellmentioning
confidence: 99%
See 1 more Smart Citation
“…Te AMJ13 cell line, derived from Iraqi patient with breast cancer cells, exhibits a lack of estrogen and progesterone receptors [24]. Tis cell line has been widely utilized in our laboratory as well as by other researchers [25][26][27][28]. Human breast cancer cell line estrogen, progesterone receptors positive (MCF-7), were provided by the Experimental Terapy Department, ICCMGR, Baghdad, Iraq.…”
Section: Cellmentioning
confidence: 99%
“…Cytotoxicity was determined by the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. Te cells were exposed to a range of diluted concentrations of papaverine (10,25,50,100,150, and 200 μm) (Santacruz Biotechnology, USA) and NDV over a range of diluted multiplicities of infection (0.1, 1, 3, 5, 10, and 20 MOI) for the determination of IC50 for both papaverine and NDV. After 72 h, the media was discarded, and each well received 50 μl of MTT dye solution (2 mg/ml) (Bio-World, USA) was incubated for three hours and solubilized with 100 μl of dimethyl sulfoxide (DMSO) (Santa Cruz Biotechnology, USA).…”
Section: Cytotoxicity Assay Amj13 Mcf-7 and Normal Cellsmentioning
confidence: 99%